Member since: 2019
Organization: Greenlight Biosciences
Great plates for filtering aqueous samples of low surface tension without leakage!
Application Area: Separate proteins and debris from cell lysates as a first step to clean-up nucleic acids
"Unlike plates with hydrophilic membranes, which usually leak profusely if the samples in the wells contain surfactants like SDS, these plates did not leak at all when 10%SDS was added and incubated for hours [and still allowed the soluble portion of my lysates to filter through with good recovery] Pre-wetting the wells with 30% ethanol increased the filtration rate but, even without pre-wetting, my aqueous suspensions filtered through the membranes fairly quickly without needing to add any organic solvents. The well volume is 0.5ml, slightly larger than the typical 96 well [~300ul], is another nice feature. Interestingly, the same manufacturer has a deep-well version [~2ml/well] made with the same materials but every time I tried to use those for the same application, they leaked badly so I ended up having to adapt my protocol to the smaller volume. I found it works very nicely so now I am a big fan of those plates!"