Member since: 2021
Application Area:Cloning to make plasmids for transfecting
"This worked very well. I used it because the first time my experiment failed and I wanted to find out where the problem was. I used these to compare and found out the comp cells we were using were not working. These ones did, though, and everything worked out after."
Member since: 2020
Organization: University of Maryland
Recommend using this.
"The item was so easy to clone plasmid with in a short time and was very handy. In addition, the outcome was reasonable compared with other companies' products. Recommend to use when you do not have enough time and still need successful results."
The Mix & Go! Competent Cells - DH5 Alpha are premade E. coli DH5 alpha competent cells for simple and highly efficient DNA transformation. These DH5 alpha competent cells are made chemically competent by a method that completely eliminates the need for heat shocking and related procedures. For transformation, simply mix DNA with cells and then spread onto solid medium − Mix & Go! The premade Mix & Go! competent cells are highly efficient (> 108 transformants/µg pUC19) and can be used for cloning, sub-cloning, PCR fragment cloning, library construction, etc.