Mini-PROTEAN TGX Stain-Free™ Precast Gels by Bio-Rad

4.9
/
5.0
  |  4 reviews
Mini-PROTEAN TGX Stain-Free precast gels for polyacrylamide gel electrophoresis (PAGE) allow for fast run times, high transfer efficiency, and rapid detection of proteins with stain-free enabled imagers. Available in six polyacrylamide gel percentages an


Mini-PROTEAN TGX Stain-Free™ Precast Gels by Bio-Rad product image
Mini-PROTEAN TGX Stain-Free Precast Gels
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Average Rating: 4.9
4 Scientists have reviewed this product

5 out of 5
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money


  • Status:

    Reviewer
  • Member since: 2016

  • Organization: Goverment VITO



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great improvement comparing previous versions
Rating: 5.0

  • Application Area: Blotting proteins

"I have very nice results with this system, extremely reliable, and easy to recover the protein blots"

Review date: 29 Jul 2016 | 10% Mini-PROTEAN® TGX Stain-Free™ Protein Gels, 15 well, 15 µl
  • Status:

    Reviewer

  • Member since: 2015

  • Organization: gemapharma



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
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Rating: 4.7
"It's really effective, durable and easily handled. It helps me to quicken and finish my work. "

Review date: 24 Jul 2015 | 10% Mini-PROTEAN® TGX Stain-Free™ Protein Gels, 15 well, 15 µl
  • Status:

    Reviewer

  • Member since: 2005

  • Organization: North Eastern University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Rating: 5.0

  • Application Area:Protein Analysis In PAGE And Western Blot

"This gel has a long shelf-life, is easy to use, provides a great separation in a short time and relative not very expensive. "

Review date: 07 Nov 2014 | 10% Mini-PROTEAN® TGX Stain-Free™ Protein Gels, 10 well, 30 µl
  • Status:

    Reviewer

  • Member since: 2014

  • Organization: DOC/Bureau of Industry & Security



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
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Rating: 4.7

  • Application Area:Protein Chemistry

"The pre-made gel is very easy to use. It has made electrophoresis extremely easy and convenient without spending hours preparing gels for an experiment. In addition, it provides consistency between experiments done at different times. "

Review date: 21 Mar 2014 | 4–20% Mini-PROTEAN® TGX Stain-Free™ Protein Gels, 15 well, 15 µl

Mini-PROTEAN® TGX Stain-Free™ Precast Gels are based on the long-shelf-life TGX (Tris-Glycine eXtended) formulation and include unique trihalo compounds that allow rapid fluorescent detection of proteins with Bio-Rad stain-free imaging systems.

Features and Benefits of Mini-PROTEAN TGX Stain-Free Precast Gels

  • Run up to 4 Mini-PROTEAN TGX Stain-Free Protein Gels using the Mini-PROTEAN Tetra Cell
  • Run times as short as 15 minutes
  • Transfer proteins in as little as 15 minutes; verify transfer to membrane with a stain-free enabled imager
  • Inexpensive Laemmli buffer system, low running costs
  • Gel images and complete analysis in less than 5 minutes after electrophoresis
  • Comparable sensitivity to Coomassie stain
  • Better reproducibility and quantitation compared to staining procedures

Applications and Uses of Mini-PROTEAN TGX Stain-Free Precast Gels

  • 1-D polyacrylamide gel electrophoresis for protein separation
  • Accurate molecular weight estimation
  • Robust system for complex samples
  • One gel for western blotting, staining, and mass spectrometry

More Information

Mini-PROTEAN TGX Stain-Free Precast Gels do not contain SDS. For standard denaturing electrophoresis use sample and running buffers containing SDS. To retain native protein conformation and activity these gels can be run with sample and running buffer that do not contain SDS.

Mini-PROTEAN TGX Stain-Free Gels retain Laemmli-like separation characteristics using standard sample and Tris/glycine running buffers. Protein migration patterns under non-denaturing conditions do not provide accurate molecular weight estimations.

The trihalo compounds in Mini-PROTEAN TGX Stain-Free Gels react with tryptophan residues in proteins in a UV-induced 1-minute reaction to produce fluorescence. The fluorophores remain covalently bound to the proteins throughout electrophoresis and blotting and may be subsequently visualized in gels or on PVDF membranes multiple times for validation in a western blotting workflow. The use of stain-free imaging also allows for the normalization of bands to the total protein on a blot, eliminating the use of housekeeping proteins or stripping and reprobing.

Ready to switch from Life Technologies NuPage or Bio-Rad’s Ready Gel Precast Gels? Use the the Interactive Gel Selector to find equivalent Mini-PROTEAN TGX Stain-Free Precast Gels.

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Product Overview

Mini-PROTEAN TGX Stain-Free™ Precast Gels by Bio-Rad product image

Mini-PROTEAN TGX Stain-Free™ Precast Gels

Manufacturer Bio-Rad

4.7 / 5.0 | 4 reviews