Member since: 2016
Organization: Goverment VITO
Great improvement comparing previous versions
Application Area: Blotting proteins
"I have very nice results with this system, extremely reliable, and easy to recover the protein blots"
Member since: 2015
"It's really effective, durable and easily handled. It helps me to quicken and finish my work. "
Member since: 2005
Organization: North Eastern University
Application Area:Protein Analysis In PAGE And Western Blot
"This gel has a long shelf-life, is easy to use, provides a great separation in a short time and relative not very expensive. "
Member since: 2014
Organization: DOC/Bureau of Industry & Security
Application Area:Protein Chemistry
"The pre-made gel is very easy to use. It has made electrophoresis extremely easy and convenient without spending hours preparing gels for an experiment. In addition, it provides consistency between experiments done at different times. "
Mini-PROTEAN® TGX Stain-Free™ Precast Gels are based on the long-shelf-life TGX (Tris-Glycine eXtended) formulation and include unique trihalo compounds that allow rapid fluorescent detection of proteins with Bio-Rad stain-free imaging systems.
Features and Benefits of Mini-PROTEAN TGX Stain-Free Precast Gels
Applications and Uses of Mini-PROTEAN TGX Stain-Free Precast Gels
Mini-PROTEAN TGX Stain-Free Precast Gels do not contain SDS. For standard denaturing electrophoresis use sample and running buffers containing SDS. To retain native protein conformation and activity these gels can be run with sample and running buffer that do not contain SDS.
Mini-PROTEAN TGX Stain-Free Gels retain Laemmli-like separation characteristics using standard sample and Tris/glycine running buffers. Protein migration patterns under non-denaturing conditions do not provide accurate molecular weight estimations.
The trihalo compounds in Mini-PROTEAN TGX Stain-Free Gels react with tryptophan residues in proteins in a UV-induced 1-minute reaction to produce fluorescence. The fluorophores remain covalently bound to the proteins throughout electrophoresis and blotting and may be subsequently visualized in gels or on PVDF membranes multiple times for validation in a western blotting workflow. The use of stain-free imaging also allows for the normalization of bands to the total protein on a blot, eliminating the use of housekeeping proteins or stripping and reprobing.
Ready to switch from Life Technologies NuPage or Bio-Rad’s Ready Gel Precast Gels? Use the the Interactive Gel Selector to find equivalent Mini-PROTEAN TGX Stain-Free Precast Gels.
Mini-PROTEAN® TGX™ Precast Gels
Mini-PROTEAN Tetra Cell
Gel opening lever (4560000), sold separately, is 100% aluminum and recyclable