microOptilab Refractive Index Detector for UHPLC by Wyatt Technology Corp.

The microOptilab^® differential refractive index (dRI) detector is specifically designed for use with any UHPLC system. It works alone as a universal concentration detector, or in combination with the µDAWN^® multi-angle light scattering (MALS) detector to measure absolute molar mass and size of macromolecules and nanoparticles in solution, via µSEC-MALS. 

Considering the extremely small peak volumes of UHPLC, band broadening in the microOptilab is kept to the absolute minimum, at less than 4 µL, as compared to 20-40 µL or more from other dRI detectors. 

Features: 

• dRI noise: 1.5x10^-9 RIU
• dRI range: ±4.7x10^-3 RIU
• Dynamic range: 6,000,000:1
• Absolute RI sensitivity: ±0.002 RIU
• Absolute RI range: 1.2 – 1.8 RIU
• Temperature control: heated/cooled 4 - 65°C, including active stabilization—even at room temperature 
• Wavelength: 660 nm, matched to the µDAWN; other wavelengths available 
• Touch-panel display for immediate access to data, diagnostics and settings

Applications: 

• Universal online concentration measurement for chromatography, especially µSEC-MALS for absolute molecular weight
• Analysis of conjugated proteins, co-polymers and nanoparticle-encapsulated drugs/proteins/DNA/RNA when combined with a MALS and UV detector