microDAWN® by Wyatt Technology Corp.

Manufacturer Wyatt Technology Corp.  |   Model: WUDAWN  |  Available Worldwide
5.0
/
5.0
  |  1 reviews
The µDAWN determines absolute molecular weight and size of proteins, polymers and other macromolecules in conjunction with UHPLC.


microDAWN® by Wyatt Technology Corp. product image
microDAWN®

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Average Rating: 5.0
1 Scientist has reviewed this product

5 out of 5
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money


  • Status:

    Expert Reviewer
  • Member since: 2015

  • Organization: Ajinomoto Althea



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Wonderful instrument, supports daily activities.
Rating: 5.0

  • Application Area: Determine Mw of samples after UHPLC separation

"Advances in UHPLC technology have greatly reduced run times, sample loading requirements, and solvent consumption. The µDAWN seamlessly integrates downstream from our Agilent 1290 UHPLC for accurate measurements of Mw, Rz and Rh values, giving us a deeper look into our sample beyond just the UV chromatogram. It is just as easy to use as our other Wyatt MALS detectors and is used almost daily."

Review date: 18 May 2017 | microDAWN®

The µDAWN is the only multi-angle light scattering (MALS) detector that can be coupled to any UHPLC-SEC system without loss of resolution.  µSEC-MALS determines absolute molecular weight, size and conformation of polymers, peptides, and proteins directly, without resorting to column calibration or reference standards. 

Features: 

  • Sensitivity: 3.5 ng of 100 kDa polystyrene in THF or 70 ng of BSA in PBS, loaded on a 4.6 x 150 mm UHPLC-SEC column. 
  • Range of molar mass: 200 Da to 10 MDa (may vary with molecular composition, size or solvent) 
  • Range of rms radius Rg: 10 nm to 50 nm 
  • Range of hydrodynamic radius: 0.5 nm to 30 nm (with optional WyattQELS® embedded dynamic light scattering module)
  • Integrated ultrasonic flow cell cleaning module 
  • Software: ASTRA® chromatography software provides comprehensive analyses and user-friendly features including HPLC control, “One-click MW” and user-designed reports. Optional 21CFR11 support for GMP. 

Applications:

  • Proteins: absolute molar mass, size and stoichiometry of monomers, oligomers, complexes, aggregates and fragments.
  • Glycoproteins, detergent-solubilized membrane proteins, PEGylated proteins, protein-polysaccharide and protein-DNA complexes; heme-containing proteins that absorb laser light
  • Protein-protein and protein-nucleic acid interactions
  • Polymers: absolute molar mass and size (distributions and moments), conformation, and branching
  • Linear or branched polymers, co-polymers
  • Most aqueous and organic solvents