Member since: 2017
Organization: University of St Andrews
Very good instrument, fast and easy to use.
Application Area: Binding experiments with macromolecules (proteins, peptides)
"The instrument is very easy to use, there are videos demonstrating everything you need to use it. Loading is a bit difficult, but because it uses very little material it is not a huge deal to miss a few injections until you get used to loading efficiently. In one day it is possible to get 5 or 6 experiments done, if not more - so very fast compared to other ITC instruments. I find it very reproducible as well. I am overall very happy with the instrument, since I was able to obtain data in a really short time, including binding and kinetic experiments. The only issue I have with it is that the service contract is on the expensive side. It is possible to do routine maintenance without service (there are also videos for that), and Malvern has good phone technical service so overall it is not a major set back since most service contracts are expensive."
Manufacturer's ResponseThank you very much for your feedback
The MicroCal PEAQ-ITC Automated is a configurable, low volume, highly sensitive isothermal titration calorimeter which offers the convenience of unattended operation. It delivers direct, label-free in solution measurement of all binding parameters in a single experiment. Applications include characterizing molecular interactions of small molecules, proteins, antibodies, nucleic acids, lipids and other biomolecules. It can also be used to measure enzyme kinetics.
The system directly measures heat released or absorbed during biochemical binding events, from which it calculates binding affinity (KD), stoichiometry (n), enthalpy (ΔH), and entropy (ΔS). Other than preparing samples and reagents to appropriate concentrations, there is minimal assay development and time to first result is fast. Easy-to use software includes optimized automation scripts for robust operation with a variety of sample types and conditions.