EMD Millipore's Mesenchymal Stem Cell Characterization Kit (Catalog Number SCR018) contains a panel of positive and negative selection markers for the characterization of the mesenchymal stem cell population in rat. Positive cell markers include antibodies directed against cell-surface molecules (integrin beta1 and CD54) (Pittenger, M. F., and Marshak, D. R., & Pittenger, M.F., et al.) present on mesenchymal stem cells along with two extracellular matrix molecules (fibronectin and type I collagen) that are synthesized by cultured mesenchymal stem cells (Prockop, D. J.). Along with the positive selection markers, two specific hematopoietic cell surface markers, (CD14 - present on leukocytes and CD45 - present on monocytes and macrophages) are provided whose expressions should not be present on mesenchymal stem cells (Pittenger, M. F., and Marshak, D. R., & Alhadlaq, A., and Mao J. J, & Pittenger, M.F.,et al.). Mouse and rabbit immunoglobulins for the assessment of background staining are also included.
For Research Use Only; not for use in diagnostic procedure.