MagicPrep™ NGS system

In this application note, Tecan demonstrates the use of the PacBio SMRTbell® prep kit 3.0 and DreamPrep NGS Compact platform to automate the generation of DNA sequencing libraries. It shows that running automated workflows with minimal user interaction delivers high-quality and consistent libraries, allowing staff to undertake more meaningful work instead of performing long repetitive protocols.

Next-generation sequencing (NGS) encompasses several modern high-throughput technologies that enable DNA and RNA to be sequenced more rapidly and efficiently than traditional Sanger sequencing.

In this expert guide, we explore a comprehensive portfolio of automated NGS library preparation solutions and show how they can be applied to a range of applications in human disease, highlighting their uses in oncology, virology, and neurology.

This eBook will also outline:

• How to simplify library preparation
• The key benefits of automated library preparation
• Fast and reliable NGS solutions to ensure optimal performance in every laboratory

In this application note, learn more about the MagicPrep™ NGS system by Tecan. explore how this system is an ideal solution for saving time by automating the generation of amplicon-based libraries. The workflow may be used with Revelo™ DNA-Seq Enz for MagicPrep NGS for large amplicon sizes (>300 bp) or with Revelo™ DNA-Seq Mech for MagicPrep NGS for smaller amplicon sizes (<300 bp). Running the instrument is a 1-step process allowing for 5.5 hours of uninterrupted walkaway time. This time savings of 9x over standard manual workflows provide lab scientists ample time to perform other tasks, including preparing the next set of samples. 

The Tecan MagicPrep™ NGS system is a unique solution for fully automated preparation of next generation sequencing (NGS) libraries. In this application note, learn how the system combines hardware, software, reagents, and consumables to provide a complete solution. After an initial 10-minute setup, the user can walk away and come back to finished NGS libraries. The instrument simultaneously produces eight libraries, providing an automated solution for sequencing library preparation for mid- to low-throughput laboratories. Discover through a case study of the Revelo™ DNA-Seq Mech workflow, how low input amounts can be used on the MagicPrep NGS system.

Routine manual preparation of genomic libraries is a process that takes up to ~5 to ~7.5 hours of personnel time. In this poster, learn more about MagicPrep™ NGS, a hands-free, walkaway solution for saving time by automating the preparation of both DNA and RNA libraries. The generated libraries are readily sequenced on Illumina® platforms. However, these libraries can be directly converted, without amplification, to Element AVITI™-ready samples via the Adept™ Library Compatibility Kit v1.1 with no reduction in library quality. Explore the comparion of libraries generated from MagicPrep NGS on the Illumina MiniSeq™ platform against the Element Biosciences AVITI System. The AVITI sequencing reads are comparable to MiniSeq reads across all metrics, highlighting the potential for automated Element Biosciences library preparation with MagicPrep NGS.

Routine manual preparation of genomic libraries is a process that consumes between ~5 to ~7.5 hours prior to sequencing. In this poster, learn about MagicPrep™ NGS, a hands-free, walkaway solution for saving time by automating the preparation of both DNA and RNA libraries. Explore how generated libraries are readily sequenced on Illumina platforms. However, these libraries can be directly converted, to G4-read samples through PCR of the Singular Genomics adapters. This poster compares libraries generated from MagicPrep NGS on the Illumina MiniSeq™platform against the Singular Genomics G4 Platform. The sequencing results delivered by the G4 platform are comparable to the MiniSeq reads across all metrics, highlighting the potential for automated Singular Genomics library preparation with MagicPrep NGS.

MagicPrep™ NGS* system is a complete automated solution for NGS library preparation, that just works. Now anyone can produce high-quality, reproducible NGS libraries with >99% mean success rate** at just a touch of a button.

*For Research Use Only. Not for use in diagnostic procedures.

** Success rate is based on mean internal data. Sample quality and variability may have an impact on the success rate.

Laboratory automation has typically been geared to high-throughput labs and requires considerable expertise to utilize efficiently. Consequently, automation has not been accessible or affordable for lower or medium throughput labs.

In this application note, meet the MagicPrep™ NGS* system, Tecan’s solution to address these issues and provide a simple solution for low-throughput automated library preparation for Illumina sequencing platforms.

* For Research Use Only. Not for use in diagnostic procedures.

T-cells are increasingly recognized for their role in SARS-CoV-2 infection and immunity, and the exploration of cellular responses in the context of COVID-19 is gaining importance for potential improvements in patient management and vaccination guidance.

Recent studies of T-cell responses have demonstrated that they can provide valuable insights into SARS-CoV-2 infection and how our immune system responds to it.

Join Dr. Rory de Vries as he presents the latest findings in the field of SARS-CoV-2-specific T-cell responses after infection or vaccination in immunocompromised populations.

In this live webinar, Dr. de Vries will address current research and discuss potential clinical utilities of T-cell immune monitoring, in the context of natural SARS-CoV-2 infection and vaccination.

Key learning objectives

• Understand the role of humoral and cellular immunity in COVID-19 natural infection and vaccination contexts
• Gain insight into the potential role of T-cell responses to emerging variants
• Become familiar with laboratory methods of cellular immunity assessment and monitoring
• Understand the potential role of cellular immunity assessment in clinically vulnerable populations

Who should attend?

• Researchers
• Physicians
• Undergraduate and postgraduate students

Certificate of attendance

All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.

Tailored Antibacterials and Innovative Laboratories for phage (Φ) Research (TAILΦR) is a Baylor College of Medicine initiative aiming to deliver personalized and effective treatments for challenging antibiotic-resistant bacterial infections.

In this presentation, Dr. Austen Terwilliger, Director of Operations for TAILΦR Labs, explores how next-generation sequencing (NGS) has become the platform of choice for constructing and characterizing bacteriophage libraries for phage therapy of human infections.

Here, Terwilliger presents a brief overview of a phage therapy pipeline, outlines potential outcomes for some patients who received phage cocktails, and details how the Tecan MagicPrep™ NGS platform is designed to shorten turnaround time and aid in analyzing purified preparations.

This talk was presented, in partnership with Tecan, at SLAS2023.

In this video, Tecan introduces the MagicPrep™ NGS system, a new category of automated laboratory equipment that provides push button simplicity for NGS library preparation for Illumina sequencing platforms.

NGS workflow with MagicPrep and the AVITI system

Hand-free library preparation to the G4 sequencing platform with the MagicPrep NGS system

Competition for NGS scientists to win a MagicPrep NGS system

Catch up on some of the most exciting announcements from analytica 2022