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Luna® Universal qPCR Master Mix

Rapid, sensitive and precise dye-based qPCR detection and quantitation of target DNA and cDNA sequences

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New England Biolabs Inc.

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Average Rating 4.7

|6Scientists have reviewed this product

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Easy to use, price is right, enzyme survived thawing.

 

Average Rating 5.0

Application Area:

Testing for virus in insects

Easy to use, beautiful data, robust enzyme. I accidentally left the enzyme mix in an ice bucket overnight, and it still worked the next day.

Review Date: 16 Nov 2021 | New England Biolabs Inc.

Gives great results.

 

Average Rating 5.0

Application Area:

qPCR

Easy and amazing to use! Simple to follow, with minimal room for error.

Review Date: 16 Jul 2021 | New England Biolabs Inc.

Excellent quantification by using this kit.

 

Average Rating 4.7

Application Area:

Rare gene amplification and quantification

I’ve found this kit recently to be more reliable and more reproducible compared to our other quantification kits. It has easy to follow instructions and provides reliable and highly reproducible results. This kit is superb for gene quantification, but simple and straightforward, highly recommend using in the molecular biology experiments specially for gene quantifications.

Review Date: 10 Jan 2021 | New England Biolabs Inc.

Highly sensitive and great results

 

Average Rating 4.7

Application Area:

Detect COVID-19 positives

Excellent one step RT-PCR kit master mix to be use in a recent, hot ongoing research of SARS COVID-19 detection. Very straightforward to use to make qPCR reactions, simply use this one step master mix with reverse transcriptase enzyme and primers. This kit has high sensitivity for the detection even when the sample has a very low intensity. Very useful kit for this type of experiment. Highly recommended.

Review Date: 17 Dec 2020 | New England Biolabs Inc.

Excellent product, well worth trying.

 

Average Rating 4.3

Application Area:

qPCR to determine absolute telomere length

This product was used as a replacement for an established method to determine aTL via qPCR. The results were excellent. I observed high amplification efficiency without having to optimize the temperature cycling conditions. The results were replicable between runs for both the target as well as single copy control genes.

Review Date: 1 Jul 2020 | New England Biolabs Inc.

Great product if you optimize your reaction.

 

Average Rating 4.7

Application Area:

DNA copy number quantificaiton

This is very sensitive (~3 cycles earlier Ct than the product we used to use) and also blue color makes it easy to trace the reaction set up. But it also amplifies primer dimer very quickly, so you need to optimize the reaction first.

Review Date: 28 Mar 2017 | New England Biolabs Inc.

Rapid, sensitive and precise dye-based qPCR detection and quantitation of target DNA and cDNA sequences.

Make a simpler choice

  • One product per application simplifies selection
  • Convenient master mix formats and user-friendly protocols simplify reaction setup
  • Non-interfering, visible tracking dye helps to eliminate pipetting errors

Experience best-in-class performance

  • All Luna® products have undergone rigorous testing to optimize specificity, sensitivity, accuracy and reproducibility
  • Products perform consistently across a wide variety of sample sources
  • A comprehensive evaluation of commercially-available qPCR and RT-qPCR reagents demonstrates superior performance of Luna products

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