Leica TCS SP8 STED by Leica Microsystems Europe
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Status:
Advanced Reviewer
Member since: 2016
Organization: University of Buenos Aires
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- Ease of use
- After sales service
- Value for money
Fast imaging with this microscope
Application Area: High-resolution microscope
"This microscope is fully motorized, easy to use and with appropriate reagents, STED can be performed and great resolution achieved. Service is always there to care for us."
Review date: 22 Nov 2017 | Leica TCS SP8 STED
Status:
Advanced Reviewer Member since: 2015
Organization: University of Manchester
-
- Ease of use
- After sales service
- Value for money
The new gSTED offers great resolution improvement in xy and z planes
Application Area:Super resolution fluorescence imaging
"We have the new (summer 2015) gated STED. Brilliant microscope for imaging fixed samples with greatly enhanced resolution in xy and z. The new gSTED is a huge improvement on the earlier versions which bleach the sample. My lab loves this microscope!"
Review date: 09 Sep 2016 | Leica TCS SP8 STED
The Leica TCS SP8 STED super-resolution microscope allows you to discover more and provides fast, intuitive, purely optical access to structural details smaller than 50 nm.
Studying subcellular architecture and dynamics with conventional fluorescence microscopy can be challenging as structures smaller than 200 nm are lost. Stimulated Emission Depletion (STED) microscopy is a stunningly simple way to overcome the diffraction limit - where fluorescence is generated is scaled down to sub-diffraction size. Stimulated emission switches off the ability of the dye to fluoresce in the periphery of the excitation spot yielding confocal super-resolution pixel by pixel.
STED microscopy does not rely on mathematics. What you see is what you get. Of course, results can be improved further by deconvolution. Just one click instantly switches from confocal to super-resolution, even during a live scan. Within seconds, imaging parameters can be optimized and super-resolved images obtained.
Leica TCS SP8 STED Benefits:
- Resolve Details Smaller than 50 nm Quickly and Directly - Just one click instantly switches from confocal to super-resolution, even during a live scan. Within seconds, imaging parameters can be optimized and super-resolved images obtained.
- Study Dynamics in Living Cells at the Nanoscale - STED microscopy minimizes the effect of sample drift and combines intrinsic optical sectioning capability and fast image acquisition.
- Perform Colocalization Studies with Dual Color STED Imaging - immediate access to dual color super-resolution images. The same STED doughnut is used for both quasi-simultaneously acquired colors. This empowers colocalization studies at the nanoscale without the need to compensate for chromatic aberrations or sample drift.
- Enjoy the Freedom to Choose Popular Fluorescent Dyes and Proteins - STED microscopy works with standard fluorophores such as Alexa 488, Oregon Green 488 and FITC and does not rely on oxygen depletion or reducing agents in the embedding medium. Also eYFP, Citrin and eGFP yield convincing results.
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