STELLARIS FALCON FLIM Microscope
STELLARIS 8 FALCON (FAst Lifetime CONtrast) is the future of functional imaging. Harness the power of fluorescence lifetime to investigate cellular physiology and explore dynamics in living cells.
Histological section from cat eye
Receive your quote directly from the manufacturer.
The contrast is clear. Lifetime imaging in an instant.
STELLARIS 8 FALCON (FAst Lifetime CONtrast) is the future of functional imaging. Harness the power of fluorescence lifetime to investigate cellular physiology and explore dynamics in living cells. STELLARIS 8 FALCON is a fully integrated solution for Fluorescence Lifetime Imaging (FLIM) and enables video-rate lifetime imaging acquisition for rapid kinetic studies in live cells.
STELLARIS 8 FALCON adds a new dimension of contrast to your imaging, opening the door to biosensing and tracking of interactions between proteins. FLIM information is now available for all modalities of the STELLARIS 8 system.
Now you can:
- Follow fast molecular interactions via FLIM-FRET (Förster Resonance Energy Transfer)
- Use biosensors to detect changes in metabolic state and microenvironment
- Apply lifetime contrast to separate multiple fluorophores
- Acquire FLIM data with minimal training
Brochures
STELLARIS Confocal Microscope
Leica Microsystems’ family of confocal microscopes – STELLARIS 5 and STELLARIS 8 – takes confocal imaging to a new level. Now you can see more, discover more, and do more than ever before, with STELLARIS.
A new world of confocal applications with the next-generation white light lasers
As biological questions get more complex, there is an increasing need to study multiple events simultaneously in the same specimen. When preparing the specimen for imaging experiments, this need is translated in having to use multiple fluorescent labels. Unfortunately, the visualization of multiple events with confocal microscopy is limited by the number of fluorescent probes that can be simultaneously imaged without incurring bleed-through artifacts due to significant spectral overlap. Using a confocal microscope with an integrated White Light Laser (WLL) enables tuning excitation and detection to perfectly match the spectral profile of the dyes in your sample, giving you the flexibility to perform multicolor experiments while obtaining clear, sharp images.
In this application note find out how to:
- Achieve complete wavelength freedom thanks to the unique combination of high-efficiency pulsed WLL excitation, AOBS technology, and spectral detection.
- Get more flexibility when designing your experiment with an expanded WLL spectrum.
- Use fluorescence-lifetime-derived information delivered by TauSense and increase the number of fluorescent probes which can be resolved in a single sample.
Quantify meaningful changes in the metabolic status of single cells
Metabolic imaging based on fluorescence lifetime provides insights into the metabolic dynamics of cells, but its use has been limited as expertise in advanced microscopy techniques was needed. Now, STELLARIS 8 DIVE FALCON makes metabolic imaging accessible to every scientist, thanks to the integration of phasor analysis to easily use fluorescence lifetime information.
FLIM phasor analysis is a robust analytical method to generate a visual fingerprint of the lifetime of a fluorophore that can be mapped back onto an image to highlight function in cells. Download this application note for a summary of studies that use FLIM phasor analysis to reveal connections between cellular metabolism and pivotal cellular functions in health and disease.
Exploring hidden dimensions of cell and tissue structure and physiology with fluorescence lifetime imaging (FLIM)
Learn how FLIM adds information depth to confocal experiments and reveals novel insights that are difficult or impossible to discover with conventional intensity-based analysis.
