STELLARIS Confocal Microscope Platforms

As biological questions get more complex, there is an increasing need to study multiple events simultaneously in the same specimen. When preparing the specimen for imaging experiments, this need is translated in having to use multiple fluorescent labels. Unfortunately, the visualization of multiple events with confocal microscopy is limited by the number of fluorescent probes that can be simultaneously imaged without incurring bleed-through artifacts due to significant spectral overlap. Using a confocal microscope with an integrated White Light Laser (WLL) enables tuning excitation and detection to perfectly match the spectral profile of the dyes in your sample, giving you the flexibility to perform multicolor experiments while obtaining clear, sharp images.

In this application note find out how to:

• Achieve complete wavelength freedom thanks to the unique combination of high-efficiency pulsed WLL excitation, AOBS technology, and spectral detection.
• Get more flexibility when designing your experiment with an expanded WLL spectrum.
• Use fluorescence-lifetime-derived information delivered by TauSense and increase the number of fluorescent probes which can be resolved in a single sample.

To address the specific challenges of live cell confocal imaging and help researchers get closer to the truth, Leica Microsystems has re-imagined confocal microscopy, designing its entirely new STELLARIS platform from the ground up. Here, explore how STELLARIS unlocks the full power and potential of live cell studies by overcoming many common limitations and fully integrating fluorescence lifetime-based information to add a new dimension to your research.

• Image longer without photodamage, and with enhanced sensitivity for the fluorophores you use most.
• Study dynamic events at exceptionally high frame rates—capturing up to 428 frames per second without compromising spatial resolution.
• Multiplex beyond spectral options, resolving overlapping fluorescence spectra, and adding the fluorescence lifetime dimension to your experiments.

Leica Microsystems’ TauSense technology is a set of imaging tools based on fluorescence lifetime. Found at the core of the STELLARIS confocal platform, it will revolutionize your imaging experiments. Whatever your sample or staining procedure, fluorescence lifetime information is always there. Now TauSense gives you access to this additional information and expands the potential of your research with the possibilities provided by different TauSense modes.

In this application note, find out how you can use TauSense technology to:

• Explore a new dimension of information in every confocal experiment with instant access to functional information.
• Improve image quality by removing unwanted fluorescence signals that mask your data and prevent you from truly seeing your sample.
• Separate fluorescent species based on lifetime information, allowing you to distinguish fluorescent signals with similar spectra.

In this technical note learn how the new Leica TCS SP8 DLS makes light sheet microscopy easier. The invention of the unique TwinFlect mirror, which deflects the illuminating light sheet at a 90° angle, allows the integration of the light sheet module into the vertical axis of every inverted Leica TCS SP8 without compromising confocal functionality.

We speak with Leica Microsystems to gain a sneak peek at their latest microscopy products on display at analytica 2022. From the ‘world’s first imaging Microhub’ to cryo-immobilization of aqueous samples, discover the key features and research applications from the product experts themselves.

With the STELLARIS confocal platform, Leica Microsystems have re-imagined confocal microscopy to get you closer to the truth. Watch the video to find out how.

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