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Lambda EMBL3/Gigapack II XL Cloning Kit

The Lambda ZAP-CMV vector allows production of libraries in a high-efficiency lambda vector. Libraries cloned into lambda vectors routinely produce greater than 4 times the primary library size as plasmid libraries. The Lambda ZAP II system combines the high efficiency of lambda library construction and the convenience of a plasmid system with improved blue-white color selection.  High-efficiency lambda cloning with versatili…

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The Lambda ZAP-CMV vector allows production of libraries in a high-efficiency lambda vector. Libraries cloned into lambda vectors routinely produce greater than 4 times the primary library size as plasmid libraries. The Lambda ZAP II system combines the high efficiency of lambda library construction and the convenience of a plasmid system with improved blue-white color selection.

  • High-efficiency lambda cloning with versatility of plasmid
  • in vivo excision of cloned fragments into phagemid vector
  • Expression of fusion proteins under control of lac promoter
  • Allows screening in lambda with DNA probe or in mammalian cells with functional screen
  • Create large, highly-representational libraries
  • Clone genes that are toxic in E.coli

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