Lactose Assay Kit by Merck

Manufacturer Merck
  |  1 reviews
Sufficient for 100 colorimetric or fluorometric tests.

Lactose Assay Kit by Merck product image
Lactose Assay Kit

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Average Rating: 4.0
1 Scientist has reviewed this product

3 out of 5
Ease of use
5 out of 5
After sales service
4 out of 5
Value for money

  • Status:

  • Member since: 2019

  • Organization: Eurofins Advinus Ltd

  • Ease of use
    3 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
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Great results within the reported acceptance criteria.
Rating: 4.0

  • Application Area: To measure lactose content in milk

"Suitable for the direct measurement of lactose levels in various biological samples (serum, plasma, other body fluids, food, growth media, etc.)."

Review date: 13 Dec 2019 | Lactose Assay Kit

Lactose is a disaccharide formed by the condensation of one galactose and one glucose molecule. Lactose is the major sugar in the milk of most species, typically present between 2–8%. The enzyme lactase hydrolyzes lactose to its constituent monosaccharides. In neonates, glucose released via the action of lactase is a major energy source.  

In this assay, lactose concentration is determined by an enzymatic assay, in which lactose is converted to galactose and glucose. The galactose is subsequently oxidized, resulting in a colorimetric (570 nm)/ fluorometric (λex = 535 nm/λem = 587 nm) product, proportional to the lactose initially present. This kit is suitable for use with various biological samples and has a linear range of detection between 0.2–1.0 nmole lactose for the fluorometric assay and 2–10 nmoles lactose for the colorimetric assay.