The pHrodo™ Green E. coli BioParticles™ Phagocytosis Kit for Flow Cytometry enables the detection of phagocytic activity in whole blood samples and live cell lines using flow cytometry. With no need for quenching and wash steps, the kit saves time and minimizes uncertainty over particle signals from internalized particles.
The pHrodo™ Green E. coli BioParticles™ assay provides sensitive detection without the need for quenching reagents and extra wash steps, helping save time and minimize the uncertainty of whether particle signals derive from internalized particles. The bioparticles carry the unique pHrodo Green dye, which is non-fluorescent at neutral pH, but turns bright green upon acidification. The pHrodo dye-based system measures phagocytic activity based on acidification of the dye as the bioparticles are ingested. To detect, the fluorophore is excited with the 488 nm argon-ion laser and fluorescence emission can be collected using a standard 530/30 nm BP filter on most flow cytometers, making it is easily multiplexable with reagents used on other lasers.