Member since: 2021
Organization: Rega Institute for Medical Research
Robust results! Best media to culture intestinal organoids!
Application Area: Proliferation media for human intestinal stem cells
"Medium is easy to prepare and use. Achieved reproducible growth between different passages of intestinal organoids. With no doubt is better than using in house medium. However, the high price of the medium is a disadvantage."
IntestiCult™ Organoid Growth Medium (Human) is a complete cell culture medium for efficient establishment and long-term maintenance of intestinal organoids derived from human intestinal crypts. Intestinal organoids provide a convenient in vitro organotypic culture system for studying the intestinal epithelium. The organoids incorporate a functional lumen enclosed by a polarized intestinal epithelial cell layer. Isolated intestinal crypts rapidly form organoids when cultured in IntestiCult™ Organoid Growth Medium (Human). Applications of intestinal organoid cultures include studying the development and function of intestinal epithelium, modeling intestinal diseases, and screening molecules for both efficacy and toxicity in an intestinal model. Intestinal organoid cultures can also be used for investigation of adult stem cell properties and for regenerative therapy approaches.