The Incucyte® Annexin V Reagents are highly-selective phosphatidylserine (PS) cyanine fluorescent dyes. Specially formulated, these reagents are ideally suited for real time measurements of apoptosis in living cultures. They are validated for use with the Incucyte® live-cell analysis system and employ simple mix-and-read protocols. Addition of the Incucyte Annexin V Reagents to normal healthy cells is non-perturbing to cell growth and morphology and yields little or no intrinsic fluorescent signal.
Once cells become apoptotic plasma membrane PS asymmetry is lost. PS exposure on the extracellular surface enables binding of the Incucyte® Annexin V Reagent resulting in a bright and photostable fluorescent signal. With the Incucyte® integrated analysis software fluorescent apoptotic cells can be quantified and background fluorescence minimized.