Member since: 2017
Easy to use
Application Area: Mutant deletion cloning
"Makes design and workload much easier. Really like the online tool."
Member since: 2016
Organization: University of Buenos Aires
Great cloning strategy
Application Area:Molecular Biology
"This kit turns out to be very easy to use. It saved the cloning of a difficult and long cDNA that we needed and couldn't clone with regular methods."
Member since: 2016
Organization: Brigham and Women's Hospital, HMS
Great results with the In-fusion cloning kit
Application Area:Plasmid cloning
"It was very simple and effective to use. It’s easy to design and clone. It works like a magic mixture. "
Member since: 2009
Organization: University of Milan Bicocca
"This kit is perfect for cloning larger fragment up to 4kb in first attempt. I am using this kit from last year and it’s perfect for cloning larger construct. The sensitivity is amazing and efficiency of positive clones is above 70%. I highly recommend because of its capability and sensitivity, saved lot of time as compared to normal cloning and decreases repetitive experiment as indirectly save cost as well. When I started using it was new to me as I failed with other cloning product at last I came up with this kit and worked very well since. "
Member since: 2015
Organization: University Freiburg
Application Area:Gateway-cloning of BCR constructs
"Cloning on the principle of a fire-and-forget system. Fast and easy application in combination with efficiencies of <90% positive clones. Worth to change.... "
PCR Cloning Kit-Seamless DNA Cloning with In-Fusion HD Cloning Plus
In-Fusion PCR Cloning Kits enable sequence independent, directional, and seamless cloning of ANY PCR fragment-or multiple fragments-into ANY linearized vector at ANY locus in a single 15-minute reaction. No additional treatment of the PCR fragment is required (such as restriction digestion, ligation, phosphorylation, or blunt-end polishing). The efficiency of In-Fusion HD Cloning Kits is over 95% across a broad insert size range (0.5 to 15 kb inserts). The In-Fusion Enzyme premix fuses PCR-generated sequences and linearized vectors efficiently and precisely, utilizing a 15 bp overlap at their ends. This 15 bp overlap can be engineered by designing custom primers for amplification of the desired sequences. This method can be used to clone single or multiple fragments into a single vector without sub-cloning.
In-Fusion HD Plus is a complete cloning solution that includes all the reagents required for successful cloning including the fast and high fidelity CloneAmp HiFi PCR Premix, the ultra-high efficiency Stellar competent cells, and a choice of PCR clean-up or gel extraction kit for DNA purification, along with the In-Fusion HD enzyme premix and reagents for control experiments as standard.