In-Fusion® HD Cloning Plus PCR Cloning Kits by Takara Bio

Manufacturer Takara Bio  |   Model: 6389  |  Available Worldwide
4.9
/
5.0
  |  6 reviews
PCR Cloning Kit-Seamless DNA Cloning with In-Fusion HD Cloning Plus


In-Fusion<sup>®</sup> HD Cloning Plus PCR Cloning Kits by Takara Bio product image
In-Fusion® HD Cloning Plus PCR Cloning Kits

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Average Rating: 4.9
6 Scientists have reviewed this product

5 out of 5
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money


  • Status:

    Reviewer
  • Member since: 2017

  • Organization: caltech



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Easy to use
Rating: 5.0

  • Application Area: Mutant deletion cloning

"Makes design and workload much easier. Really like the online tool."

Review date: 12 Dec 2017 | In-Fusion® HD Cloning Plus PCR Cloning Kits
  • Status:

    Advanced Reviewer

  • Member since: 2016

  • Organization: University of Buenos Aires



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great cloning strategy
Rating: 5.0

  • Application Area:Molecular Biology

"This kit turns out to be very easy to use. It saved the cloning of a difficult and long cDNA that we needed and couldn't clone with regular methods."

Review date: 06 Feb 2017 | In-Fusion® HD Cloning Plus PCR Cloning Kits
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Brigham and Women's Hospital, HMS



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great results with the In-fusion cloning kit
Rating: 5.0

  • Application Area:Plasmid cloning

"It was very simple and effective to use. It’s easy to design and clone. It works like a magic mixture. "

Review date: 12 Jul 2016 | In-Fusion® HD Cloning Plus PCR Cloning Kits
  • Status:

    Reviewer

  • Member since: 2009

  • Organization: University of Milan Bicocca



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Rating: 5.0

  • Application Area:Cloning

"This kit is perfect for cloning larger fragment up to 4kb in first attempt. I am using this kit from last year and it’s perfect for cloning larger construct. The sensitivity is amazing and efficiency of positive clones is above 70%. I highly recommend because of its capability and sensitivity, saved lot of time as compared to normal cloning and decreases repetitive experiment as indirectly save cost as well. When I started using it was new to me as I failed with other cloning product at last I came up with this kit and worked very well since. "

Review date: 13 Oct 2015 | In-Fusion® HD Cloning Plus PCR Cloning Kits
  • Status:

    Reviewer

  • Member since: 2015

  • Organization: University Freiburg



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Rating: 5.0

  • Application Area:Gateway-cloning of BCR constructs

"Cloning on the principle of a fire-and-forget system. Fast and easy application in combination with efficiencies of <90% positive clones. Worth to change.... "

Review date: 21 May 2015 | In-Fusion® HD Cloning Plus PCR Cloning Kits
  • Status:

    Reviewer

  • Member since: 2008

  • Organization: UT Health Science Center



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
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Rating: 4.3
"The best for cloning large sequence inserts."

Review date: 10 Aug 2008 | In-Fusion® HD Cloning Plus PCR Cloning Kits

PCR Cloning Kit-Seamless DNA Cloning with In-Fusion HD Cloning Plus

In-Fusion PCR Cloning Kits enable sequence independent, directional, and seamless cloning of ANY PCR fragment-or multiple fragments-into ANY linearized vector at ANY locus in a single 15-minute reaction. No additional treatment of the PCR fragment is required (such as restriction digestion, ligation, phosphorylation, or blunt-end polishing). The efficiency of In-Fusion HD Cloning Kits is over 95% across a broad insert size range (0.5 to 15 kb inserts). The In-Fusion Enzyme premix fuses PCR-generated sequences and linearized vectors efficiently and precisely, utilizing a 15 bp overlap at their ends. This 15 bp overlap can be engineered by designing custom primers for amplification of the desired sequences. This method can be used to clone single or multiple fragments into a single vector without sub-cloning.

In-Fusion HD Plus is a complete cloning solution that includes all the reagents required for successful cloning including the fast and high fidelity CloneAmp HiFi PCR Premix, the ultra-high efficiency Stellar competent cells, and a choice of PCR clean-up or gel extraction kit for DNA purification, along with the In-Fusion HD enzyme premix and reagents for control experiments as standard.

Features:

  • Sub-cloning is unnecessary—Clone any insert, into any location, within any vector
  • Highly efficient—Over 95% efficiency demonstrated with a broad range of fragment sizes from 0.5 kb to 15 kb
  • Seamless construction—Final constructs have no extra base pairs left over (as is often the case with restriction digest or TA cloning)
  • Flexibility to clone single or multiple fragments—Clone single or multiple DNA fragments simultaneously, in a single reaction
  • Can be used to perform site-directed mutagenesis

Applications:

  • PCR cloning
  • HTP cloning
  • Multiple fragment cloning
  • Gene synthesis
  • Gene design
  • Mutagenesis
  • Domain swapping
  • Domain modification