Member since: 2007
If you know it well, this is very useful.
Application Area: Western blotting
"This membrane is very sensitive to a low amount of protein, especially useful for detecting nuclear proteins in the nuclear fraction. But we should recognize that it could be risky to use the same condition as the classic PVDF. The range of protein retention is narrow. The low voltage transfer causes the binding proteins to come off, but it is preventable by higher voltage transfer."
This Immun-Blot® LF PVDF membrane is packaged as a 28 cm wide, 3.8 m long bulk roll in an easy-to-access box. The pore size is 0.45 µm and binding capacity is 150–160 µg/cm2.
Immun-Blot LF PVDF membrane is ideal for fluorescent blotting applications because of its low autofluorescence and high protein retention properties, allowing for long exposure times without raising background and maximizing the sensitivity of the fluorescent blot imaging system.