iCE3 by ProteinSimple

Manufacturer ProteinSimple  |   Model: 004-003  |  Available Worldwide
4.7
/
5.0
  |  2 reviews
Protein charge heterogeneity analysis in 10 minutes


iCE3 by ProteinSimple product image
iCE3

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Average Rating: 4.7
2 Scientists have reviewed this product

5 out of 5
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money


  • Status:

    Reviewer
  • Member since: 2019

  • Organization: GSK



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
This system is leaps an bounds ahead of the iCE280 system.
Rating: 4.3

  • Application Area: antibody analysis

"This system is leaps an bounds ahead of the iCE280 system. It is more straight forward for prep and enables a significant reduction in start up time."

Review date: 26 Mar 2019 | iCE3
  • Status:

    Reviewer

  • Member since: 2015

  • Organization: AB



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Rating: 5.0
"The iCE3 System performs free solution IEF in a capillary column (cIEF) and detects focused protein zones using a whole column UV absorption detector that avoids disturbing these focused zones. This technology is unique in that it has the comparable resolution of traditional gel IEF but incorporates the advantages of a column based separation technology, including quantitation and automation. It is superior to conventional cIEF performed with single point detection systems, which require a lengthy mobilization phase. As a result, method development and sample analysis can be run on the iCE3 in a fraction of the time required by other technologies and our resolution and reproducibility are superior. The iCE3, with whole-column detection, completely eliminates the need for a mobilization phase. This results in a unique combination of speed, resolution and reproducibility."

Review date: 10 Aug 2015 | iCE3

iCE3™ lets you move beyond the limits of traditional protein analysis and fast-tracks your development timelines. 10-minute start to finish run times let you optimize method conditions in an afternoon. As an added bonus, you can use the same generic method for multiple molecules—no need for product-specific methods.

iCE3 gives you high resolution, quantitation and automation without the hassles that come with other capillary IEF protein separation techniques. It'll analyze your toughest samples and most challenging proteins with ease.

FAST-TRACK YOUR DEVELOPMENT TIMELINES

iCE3's 10-minute start to finish runs with the HT Cartridge lets you optimize charge heterogeneity method conditions in an afternoon. You can also use the same platform method for multiple molecules—no need for product-specific methods. That means you can standardize platform methods across product development and QC, so you'll save time and costs and get robust and reproducible data.

Protein samples are first premixed with carrier ampholytes, additives and pI markers. Samples are separated in a capillary cartridge with electrolytic tanks at each end—one tank is filled with acid (anolyte) and the other base (catholyte).

The sample mixture is injected to fill the entire capillary column. Voltage is then applied to the anolyte and catholyte tanks. This creates a pH gradient, which separates and focuses the proteins based on their pI. The whole-column UV detector monitors the entire process in the capillary in real time. The focusing time can be optimized in a single sample run, and once it's complete the separation pattern is captured and analyzed. The capillary column is then washed so it's ready to go for the next sample.