hPSC Genetic Analysis Kit by STEMCELL Technologies Inc.

Manufacturer STEMCELL Technologies Inc.  |   Model: 7550
qPCR analysis kit for detecting the majority of karyotypic abnormalities reported in human ES and iPS cells


hPSC Genetic Analysis Kit by STEMCELL Technologies Inc. product image
hPSC Genetic Analysis Kit
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The hPSC Genetic Analysis Kit contains all required components to detect the 8 most common karyotypic abnormalities reported in human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells. This qPCR-based kit enables the genetic screening of multiple human ES and iPS cell lines in a rapid and cost-effective manner, and contains enough material to analyze 20 individual samples in triplicate. It uses double-quenched probes with a 5-carboxyfluorescein (5-FAM) dye to give superior performance over other single-quenched probes.

Specifically, the kit includes qPCR Master Mix, ROX Reference Dye, Buffer and primer/probe mixes designed to detect the critical minimal regions of these 8 commonly mutated regions along with a primer/probe mix to a control location in the genome. Additionally, a genomic DNA control is provided with demonstrated normal copy number over the regions of interest.

Our online Genetic Analysis Application ( www.stemcell.com/geneticanalysisapp ) is designed to help with data analysis and interpretation: simply input qPCR data and the tool will perform statistical analyses, assist with data interpretation, and provide visual representation of the data.