HEScGRO hES cell medium is the first, animal-component free medium that is specially formulated to meet the unique requirements of human embryonic stem cell culture. No other hES cell media is available as a serum-free, ready-to-use, and complete formulation. HEScGRO has been extensively tested and proven to maintain the pluripotential nature of several hES cell lines including: MEL-1, MEL-2 and H1. This medium is fully-defined, serum-free, and animal-component free, and does not require additional supplementation to maintain cells in their pluripotent state. Mitotically inactive feeder cell layers are commonly used to support hES cell growth. PLEASE NOTE: Human feeder cells are required for successful hES cell culture. Mouse feeder cells are not recommended with this medium.