Features of the Promega HaloLink Magnetic Beads:
• Magnetic-Based: Easy and quick immobilization of HaloTag® fusion proteins with minimal loss of sample material.
• Covalent Bond: Allows stringent washing of immobilized HaloTag® fusion protein, removing nonspecific proteins and enhancing overall data.
• Multiple Applications: Immobilized HaloTag® fusion proteins can be used for protein interaction analysis, enzyme assays and purification of fusion proteins containing a protease cleavage site.
HaloLink™ Magnetic Beads from Promega provide a rapid and reliable method to covalently capture and immobilize HaloTag® fusion proteins to a paramagnetic particle. Immobilization through the HaloTag® Ligand provides for consistent, surface-directed orientation of the fusion protein, thus providing more reliable results in applications such as protein:protein interaction studies with the fusion protein. The result is a paramagnetic bead with very high binding capacity for HaloTag® fusion proteins and very low nonspecific protein binding. The paramagnetic feature allows for streamlined assay development and facilitates applications with automated assays developed on robotic platforms.
A variety of vectors for the expression of HaloTag® fusion proteins in bacterial, mammalian or cell-free systems are available.