GoTaq® qPCR Master Mix by Promega Corp.

Manufacturer Promega Corp.
4.3
/
5.0
  |  5 reviews
GoTaq® qPCR Master Mix is a new reagent system for use in real-time quantitative PCR (qPCR). The system contains BRYT™ Green, a new proprietary fluorescent DNA binding dye that exhibits greater fluorescence enhancement upon binding to double-stranded DNA than SYBR® Green I. Combined with the GoTaq® Hot Start Polymerase, optimized buffer and proprietary dye, GoTaq® qPCR Master Mix provides robust real-time PCR with increased reliability, reproducibility and sensitivity. GoTaq® qPCR Master Mix i... Read more


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GoTaq® qPCR Master Mix
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Average Rating: 4.3
5 Scientists have reviewed this product

5 out of 5
Ease of use
4 out of 5
After sales service
4 out of 5
Value for money


  • Status:

    Reviewer
  • Member since: 2019

  • Organization: University of Bern



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
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Simple to use.
Rating: 4.7

  • Application Area: Human cell lines

"Easy to use. The PCR mix (2x) gives a simple protocol and works well generally."

Review date: 15 Nov 2019 | GoTaq® qPCR Master Mix
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: DL Seeds Inc.



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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Works well and stores nicely.
Rating: 4.3

  • Application Area:Trait testing in canola

"Product was customized to our needs, making it very easy to use and works great."

Review date: 25 Oct 2019 | GoTaq® qPCR Master Mix
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Harvard Univeristy



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
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A qPCR master mix that will give good and reliable results for a variety of primers.
Rating: 4.7

  • Application Area:qPCR of cDNA

"I used this qPCR master mix for probably thousands of qPCR runs and it was so reliable and gave great results. I used it for a variety of primers and it worked consistently and gave quality and reproducible results."

Review date: 25 Oct 2019 | GoTaq® qPCR Master Mix
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: University of Southampton



  • Ease of use
    4 out of 5
    After sales service
    3 out of 5
    Value for money
    4 out of 5
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Reliable and flexible qPCR.
Rating: 3.7

  • Application Area:Gene expression

"I have used GoTaq qPCR Master Mix with the Applied Bioscience's StepOnePlus qPCR machine for quantification of gene expression and it seems to work well. The BRYTgreen dye appears to work consistently and I like the flexibility of this product compared to using hydrolysis probe assays, since I have multiple targets to assay and so buying hydrolysis probe assays would be prohibitively expensive."

Review date: 27 Jun 2019 | GoTaq® qPCR Master Mix
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: University of York



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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Great results, will buy it again.
Rating: 4.0

  • Application Area:Analyze transcripts in Drosophila tissue

"It worked perfectly and provided good quality analysis."

Review date: 27 Jun 2019 | GoTaq® qPCR Master Mix
GoTaq® qPCR Master Mix is a new reagent system for use in real-time quantitative PCR (qPCR). The system contains BRYT™ Green, a new proprietary fluorescent DNA binding dye that exhibits greater fluorescence enhancement upon binding to double-stranded DNA than SYBR® Green I. Combined with the GoTaq® Hot Start Polymerase, optimized buffer and proprietary dye, GoTaq® qPCR Master Mix provides robust real-time PCR with increased reliability, reproducibility and sensitivity.

GoTaq® qPCR Master Mix is provided as a pre-mixed, ready-to-use stabilized 2X formulation that includes all components for qPCR except sample DNA, primers and water to dilute the DNA standards. A separate tube of 100X CXR Reference Dye is included for use with instruments that require a higher level of reference dye than that in the GoTaq® qPCR Master Mix.