Freedom EVO® PCR Workstation
The Freedom EVO PCR workstation is a fully scalable, modular system offering flexible automation for PCR set-up and cycle sequencing reaction preparation.
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PCR
It is a great platform that allows flexibility during development. This system is very easy to understand how to use.
Review Date: 25 Feb 2014 | Tecan
The Freedom EVO PCR workstation offers unsurpassed flexibility to support virtually all the PCR set-up and cycle sequencing reaction preparation needs of laboratories, whatever the throughput.
Reactions may be prepared in microtubes, 96- or 384-well microplate formats, and multiple PCR plates can be processed per run. Precise and pipetting of low volumes is performed by the LiHa (liquid displacement) and/or the Air LiHa (air displacement). Reliable pipetting of template DNA, DNA primers, and other components (buffers, enzyme, nucleotides) is guaranteed for even the most sensitive amplification procedures, with a choice of four- or eight-channel liquid handling arms, or 96- and 384-multi-channel pipetting options. For maximum flexibility, a selection of pre-optimized configurations are available, as well as tailor-made PCR set-up systems designed by Tecan’s automation consultants.
Extended automation of amplification procedures can be easily achieved by integration of Tecan’s Infinite® multimode microplate readers for nucleic acid quantification and normalization, as well as additional modules such as reagent-cooling carriers and Tecan’s high capacity storage modules. Third-party devices such as microplate sealers and thermocyclers – for example, the INHECO ODTC® thermocycler – can also be integrated. Virtually any combination of additional instruments or devices is fully supported by Tecan’s Freedom EVOware® software package.
The Freedom EVO PCR workstation features effortless touchscreen operation. Sample information can be input manually, imported from a file or identified by barcode scanning, with process security ensured through Freedom EVOware Sample Tracking. The intuitive TouchTools™ PCR Wizard guides users through straightforward reaction set-up for a wide range of applications, from end-point, real-time and multiplex PCR protocols to sequencing, genotyping and gene expression methods – as well as pathogen and mutagenesis detection – helping to reduce training time and costs for research and diagnostic laboratories. After each run, data can be seamlessly transferred to a thermocycler via a USB or LIMS for further analysis.
Reliable and Flexible Purification of RNA
This application note describes a flexible automated method for purification of RNA from cells and tissue samples, provided by Tecan and MACHEREY-NAGEL. The method uses MACHEREY-NAGEL’s NucleoMag 96 RNA kit and the Freedom EVO workstation from Tecan for fast extraction of highly pure RNA.
Automated High Yield Extraction of gDNA from Plant Samples
This application note demonstrates the potential for high yield gDNA extraction using the MACHEREY-NAGEL NucleoSpin® Plant II Midi kit on a Freedom EVO® platform. Plant research is often geared towards crop improvement, and focuses on yield and robustness to pathogens and other stress factors, such as heat or draught. It is often necessary to sequence the genome, creating the need for purification of high quantities of gDNA. DNA extraction from plant material is therefore an integral step in plant research.
Fast and Efficient PCR Clean-Up with the NucleoSpin<sup>®</sup> 96 PCR Clean-Up Kit on a Freedom EVO<sup>®</sup> Platform
This application note demonstrates how MACHEREY-NAGEL’s NucleoSpin® 96 PCR Clean-up kit and Tecan’s Freedom EVO® platform can be used in combination to provide a flexible automated solution for the purification of PCR products. PCR has become a standard molecular biology tool for biological and medical research laboratories, and is used for DNA cloning, sequencing and genetic testing. For most applications, PCR products need to be purified, and this is advantageous to perform in a high throughput format.
Efficient DNA Extraction from Food and Feed using the NucleoSpin Food kit on a Freedom EVO Workstation
Food samples are very heterogeneous, and often contain substances which can impair DNA extraction, such as fats, cocoa or polysaccharides. In addition, processed foods often have a very low DNA content, which is of poor quality. Tecan and Machery-Nagel have joined forces to provide an efficient automated solution for the isolation of genomic DNA from foods, without compromising yield or purity. This application note presents the joint system work flow for the rapid extraction of genomic DNA from a variety of food and feed samples.
Getting High Yields of gDNA from Plant Samples; MACHEREY-NAGEL and Tecan
Plant research is often geared towards crop improvement, and focuses on yield and robustness to pathogens and other stress factors, such as heat or draught. It is often necessary to sequence the genome, creating the need for purification of high quantities of gDNA. MACHEREY-NAGEL has developed the NucleoSpin Plant II Midi kit to meet the demand for efficient purification of high quality DNA from a variety of plants and fungi. This application note presents a flexible automated solution for the isolation of plant genomic DNA without compromising yield or purity; a result of Tecan and MACHEREY-NAGEL have joining forces.
DNA Profiling Of Endangered Species by PCR at the Institute for Environmental Protection and Research
This application note describes how the Laboratory of Genetics at the Institute for Environmental Protection and Research (ISPRA) is using Tecan’s TouchTools PCR Wizard and Freedom EVO® liquid handling workstation to prepare samples from Testudo marginata and Testudo graeca turtles for PCR amplification and genetic analysis.
Tecan Freedom EVO® PCR Workstation - Straightforward Automation of PCR Reaction Set-Up
Looking for effortless touchscreen operation and step-by-step instructions to set up PCR reactions? The TouchTools™ PCR Wizard offers straightforward automation of PCR reaction set-up on the Freedom EVO® PCR workstation, using the high precision air displacement pipetting arm. The Wizard simplifies reaction set-up for a wide range of applications – from end-point, real-time and multiplex PCR protocols, to sequencing, genotyping and gene expression methods, as well as pathogen and mutagenesis detection – helping to reduce training time and costs for research and diagnostic laboratories. The PCR Wizard’s intuitive TouchTools interface and step-by-step instructions guide users through the setup process, allowing sample information to be entered manually, imported from a file or identified by barcode scanning. Users can quickly and easily optimize 96- and 384-well PCR plate layouts to meet specific workflow needs, allowing up to 96 Master Mixes and multiple PCR plates per run, with their own controls and dilution curves. The worktable layout for each experiment is visually displayed, outlining where all necessary reagents and labware should be placed, as well as the volume and quantity of each item required to complete the run, minimizing the risk of failed experiments. Seamless data transfer to Bio-Rad and Life Technologies real-time cyclers avoids transcription errors, and optional integrated sample tracking further increases process security.
