FLAG® Immunoprecipitation Kit by Sigma-Aldrich Supelco
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Status:
Reviewer
Member since: 2019
Organization: Karolinska Instituet
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Very efficient. We use 2 times more FLAG peptide than what's recommended on datasheet
Application Area: FLAG Immunoprecipitation
"Very efficient. We use 2 times more FLAG peptide than what's recommended on the datasheet. This significantly helped us improve the yield of eluates without interfering with Mass Spectrometry and not giving background. It's a pity that some components within the kit cannot be bought separately."
Review date: 31 Mar 2022 | FLAG® Immunoprecipitation Kit
The immunoprecipitation is performed with the ANTI-FLAG M2 affinity gel, which is a highly specific monoclonal antibody covalently attached to agarose resin. The use of affinity resin allows an efficient binding of FLAG-tagged proteins without the need for preliminary steps and calibrations. The immunoprecipitated FLAG-tagged proteins can be efficiently eluted from the resin under acidic conditions or by competition with FLAG peptides. The immunoprecipitated proteins can be detected for their size, post-translational modifications, and interactions on gel electrophoresis, and by activity assays.
Application
Browse additional application references in our FLAG® Literature portal.
Features and Benefits
- Utilizes highly specific ANTI-FLAG M2 affinity gel
- No preliminary steps or calibrations
- 3X FLAG™ peptide provides efficient and gentle elution through direct competition
Product Overview
FLAG® Immunoprecipitation Kit
Manufacturer Sigma-Aldrich Supelco | Available Worldwide
4.0 / 5.0 | 1 reviews