FLAG® Immunoprecipitation Kit by Sigma-Aldrich Supelco

Manufacturer Sigma-Aldrich Supelco  |  Available Worldwide
4.0
/
5.0
  |  1 reviews
Sigma′s FLAG Immunoprecipitation kit allows a rapid and efficient immunoprecipitation and elution of an active FLAG-tagged protein. 
The supplier does not provide quotations for this product through SelectScience. Browse these related products instead


FLAG® Immunoprecipitation Kit by Sigma-Aldrich Supelco product image
FLAG® Immunoprecipitation Kit

The supplier does not provide quotations for this particular product through SelectScience. You can search for similar products in our product directory.




Average Rating: 4.0
1 Scientist has reviewed this product

4 out of 5
Ease of use
4 out of 5
After sales service
4 out of 5
Value for money


  • Status:

    Reviewer
  • Member since: 2019

  • Organization: Karolinska Instituet



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Share this Review on LinkedinShare this Review on TwitterShare this Review on Facebook
Very efficient. We use 2 times more FLAG peptide than what's recommended on datasheet
Rating: 4.0

  • Application Area: FLAG Immunoprecipitation

"Very efficient. We use 2 times more FLAG peptide than what's recommended on the datasheet. This significantly helped us improve the yield of eluates without interfering with Mass Spectrometry and not giving background. It's a pity that some components within the kit cannot be bought separately."

Review date: 31 Mar 2022 | FLAG® Immunoprecipitation Kit

The immunoprecipitation is performed with the ANTI-FLAG M2 affinity gel, which is a highly specific monoclonal antibody covalently attached to agarose resin. The use of affinity resin allows an efficient binding of FLAG-tagged proteins without the need for preliminary steps and calibrations. The immunoprecipitated FLAG-tagged proteins can be efficiently eluted from the resin under acidic conditions or by competition with FLAG peptides. The immunoprecipitated proteins can be detected for their size, post-translational modifications, and interactions on gel electrophoresis, and by activity assays.

Application

Browse additional application references in our FLAG® Literature portal.

Features and Benefits

  • Utilizes highly specific ANTI-FLAG M2 affinity gel
  • No preliminary steps or calibrations
  • 3X FLAG peptide provides efficient and gentle elution through direct competition

Product Overview

FLAG® Immunoprecipitation Kit by Sigma-Aldrich Supelco product image

FLAG® Immunoprecipitation Kit

Manufacturer Sigma-Aldrich Supelco  |  Available Worldwide

4.0 / 5.0 | 1 reviews