FirePlex Immunoassays by Abcam plc

Manufacturer Abcam plc  |  Available Worldwide
Abcam's proprietary FirePlex particle technology is ideal for multiplexing protein targets. 


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FirePlex Immunoassays
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​​​​Abcam's proprietary FirePlex particle technology is ideal for multiplexing protein targets. The bar-coded hydrogel particles allow the bulk immobilization of capture antibody, providing enhanced binding capacity in comparison to surface-functionalization techniques. The flexibility of the hydrogel matrix reduces steric constraints on the binding while enhancing binding kinetics in a planar array format, making assay readouts possible both on flow cytometers as well as High Content Imagers.

FirePlex Immunoassays are offered in three complementary formats for convenient scale-up of samples/compounds for screening:

FirePlex-96 assay:

  • Ideal for target screen in 96-well plate format
  • pg sensitivity and 5-log dynamic range
  • Simple, straight forward workflow
  • Readout on a flow cytometer

FirePlex-384 assay:

  • Ideal for high throughput screening in 384-well plate format
  • Small sample volume of 6.25 µl
  • No-wash, two-step - automation friendly
  • Readout on High Content Imager

FirePlex-1536 assay:

  • Ideal for high throughput screening in 1536-well plate format
  • Small sample volume of 2.5 µl
  • No-wash, one-step - automation recommended
  • Readout on High Content Imager

Product Overview

FirePlex Immunoassays by Abcam plc product image

FirePlex Immunoassays

Manufacturer Abcam plc  |  Available Worldwide

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