Status:
ReviewerMember since: 2022
Organization: University of Tennessee health science center
Cost efficient and great results
Application Area:Methylation status of pluripotent genes
"Following a thorough comparison of bisulfite converting kits, the Zymo research kit proved to be both efficient and cost-effective. Furthermore, the Bisulfite Primer Seeker aided in the creation of a primer tailored to the target. I had no trouble carrying out the experiment thanks to the clear instructions that came with the kits."
Status:
ReviewerMember since: 2021
Organization: Banaras Hindu University
This works very well and we are very much satisfied with it. Exceptionally good
Application Area:Mouse embryonic development tissue
"This kit is very good for bisulphite sequencing and very are very much happy with its results. This is exclusively good as we have generated good amount of results. Accurate analysis help us to generate very good results. In my case I have used it for very tiny samples and it worked exceptionally good."
Status:
ReviewerMember since: 2022
Organization: University of Georgia
Impressive results and easy to use and worry free
Application Area:Primary cells and cell line
"I had used this back in India and the results are always consistent and reproducible. But never had a chance to test with primary and cell lines. But here I have tried on both and surprisingly the results are great and reproducible. I love it. It's amazingly simple and easy to use."
Status:
ReviewerMember since: 2022
Organization: Virginia Tech
Good performance and well designed protocol
Application Area:Single cell analysis
"Very useful product, will purchase again!"
Status:
ReviewerMember since: 2017
Organization: UCL Cancer Institute
Best kit for bisulfite conversion of DNA
Application Area:Bisulfite conversion of cfDNA for deep sequencing
"Having tested different kits for the bisulfite conversion of DNA to study DNA methylation, I found this gave the highest yield and most reproducible result. This is the gold standard kit for this type of analysis in my diagnostic lab."
Status:
ReviewerMember since: 2021
Organization: Banaras Hindu University
Great results
Application Area:Sex determination
"The product was amazing it guarantees success every time. All my reactions were successful I have had wonderful results with this kit. I am planning some new experiments on the same system because of my earlier results. As soon as I get my grant, I will order a new set of reagents from Zymo. Most importantly it was a very well-written protocol and straightforward to follow."
Status:
ReviewerMember since: 2021
Organization: UTHSC
I'm getting consistently good results
Application Area:Analyze CpG methylation in the DNA
"The kit was easy to use, the data was also reproducible. The yield efficiency is more than 95%."
Status:
ReviewerMember since: 2021
Organization: INSTITUTO MAIMONIDES INVESTIGACION BIOMEDICA CORDOBA
More effective in less time.
Application Area:Bisulfite conversion for DNA methylation analysis
"Quick, easy and effective bisulfite modification system. This improved version that considerably saves incubation time, is a great success for lovers of epigenetics."
Status:
ReviewerMember since: 2021
Organization: Johns Hopkins Medicine
Reliable products with consistent results.
Application Area:Bisulfite conversion of human DNA
"EZ DNA Methylation-Lightning™ Kit is the bisulfite conversion kit we have been using for years. It appeared that this is one of the kits producing pure converted DNA and would not affect downstream applications such as digital PCR which requires high purity of DNA. It is a bead-based method, which is much easier to use than others. We use it for different starting concentrations from 100 pg to 2000 ng. We get consistent results from this kit all the time."
Status:
ReviewerMember since: 2021
Organization: Johns Hopkins University
High quality methylation results.
Application Area:Whole genome sequencing data
"We experience very high-quality results with this product and find the results reproducible and very reliable. We have been using Zymo methylation kits for years now, first the gold kit and now we are mainly using the lighting kit. Tech support is always very helpful and responsive whenever we have questions."
Status:
ReviewerMember since: 2020
Organization: CellMax Life
Easy to use bisulphite conversion kit
Application Area:Methylation
"The lightning kit is super-easy to use, efficient and reliable resource for bisulphite conversion. Great tool for methylation research."
Status:
ReviewerMember since: 2020
Organization: Weizmann Institute of Science
Great results, easy to use.
Application Area:Study DNA methylation during early development
"Easy to use, easy to scale down to single cell platforms and automation. Great results!"
Status:
ReviewerMember since: 2019
Organization: TruMe Labs
Very easy to use with great results.
Application Area:Epigenetics
"EZ DNA Methylation-Lightning Kit is friendly to user product and provides rapid and full DNA conversion for epigenetic applications. The conversion is done in ~1h; and in our hands the quality of conversion is the same as for Gold kit. Converted DNA is then easily purified and is ready for subsequent PCR. Contrary to other conversion kits (e.g. Gold or Direct), Lighting reagent is ready-to-use and does not require solubilization of the reagent in different buffers."
Status:
ReviewerMember since: 2020
Organization: Johns Hopkins University
"Go-to" Bisulfite conversion kit.
Application Area:Bisulfite conversion
"This is our "go-to" kit for bisulfite. Fast, efficient and inexpensive. Seems to work as well or better than all other kits we've tried."
Status:
ReviewerMember since: 2020
Organization: University of Louisiana Monroe
Great product and effective.
Application Area:Methylation analysis in prostate cancer cells
"It’s easy to use but the only problem is it produces less product, requiring me to use more columns."
Status:
Advanced ReviewerMember since: 2019
Organization: Keck of USC
Amazing product!
Application Area:Analyze DNA methylation in cancer cells
"I have been using this kit for several years. All the reagents are included in the kit and ready-to use, which means the whole process can be done in two hours. The input DNA can go down to 100 pg, which is really important if my tissue samples are very limited. The output DNA is suitable for illumina DNA methylation array or next generation sequencing. The results are always reliable and reproducible."
Status:
Advanced ReviewerMember since: 2019
Organization: Keck of USC
Great kit for DNA methylation analysis
Application Area:Analyze DNA methylation
"This kit is the best one I have used for DNA bisulfite conversion. It is easy and quick. The product quality is good and reproducible. I used the product for EPIC methylation array, sequencing, and COBRA assay - the results are great."
Status:
ReviewerMember since: 2009
Organization: UNIVERSITY OF OKLAHOMA HSC
Best kit for methylation and nothing can over take this kit
Application Area:Analyze methylation pattern in cancer cell lines with response to IR
"EZ DNA methylation lightning kit is the easiest kit for the bisulfite conversion and the methylation analysis. It gives a good yield of bisulfite converted DNA and the time required for this is very less."
Status:
ReviewerMember since: 2016
Organization: IRCM
Greats results for NGS.
Application Area:Methylation analysis
"High quality and reproducible results on next generation sequencing samples. Easy to use and high recovery efficiency. "
Status:
ReviewerMember since: 2016
Organization: Kyung hee university
Good results. High reproducibility. But low yield
Application Area:Analyze methylation specific PCR
"High reproducibility but low yield. In protocol, it's possible to convert 2ug of DNA but for high effectiveness, they recommend 500ng, which is not enough to check for one time MSP for the first time. "
Status:
Advanced ReviewerMember since: 2016
Organization: InStem - National Centre for Biological Science
A must buy for beginner.
Application Area:Bisulfite conversion of DNA
"The only product which has given satisfactory yield of 100% converted DNA. PCR worked in first shot."
Status:
ReviewerMember since: 2016
Organization: Facultad de Medicina, Univesidad de la Republica Oriental del Uruguay
Excellent
Application Area:Analyze methylation in blood samples
"The product covers our laboratory's every need, in an easy and cost-effective manner. It's really simple to use and you can always rely on customer service for help"
Status:
ReviewerMember since: 2016
Organization: The University of Oklahoma Health Sciences Center
Zymo kit is the top one in epigenetics research.
Application Area:Cancer research and methylation status.
"Nothing can replace Zymo products and the accuracy that I am getting from using this kit. Cost wise and work time wise, Zymo is user friendly. Also if we face any issues, Zymo technical support and the complete manual with the troubleshooting help us to figure out the solution. When compared with some top brand company kits, Zymo never loses its own standard in methylation and other molecular research. Due to its uniqueness and result accuracy, it's always good to stand with Zymo products. I am even an active user of some more of their products like Direct-zol™ RNA MiniPrep and DNA Clean & Concentrator™, which are always excellent products for research use. In epigenetics, ZYMO IS THE BEST AND WILL BE THE BEST, which you can trust blindly. I always strongly recommend to my colleagues to go for Zymo products for epigenetics work. Zymo always holds its name "The Epigenetics Company". I have no negative comments or negative reviews for Zymo products. Until now, I have never contacted technical support of Zymo because I have never faced any big issue in working with Zymo kits. MiniPrep kit is extremely easy to use and time saving. Compared to other products it is cheaper and less time consuming. I have also had very good results isolating different RNA sizes compared to conventional methods with trizol and, importantly, I always got the best purity. The microelution was key here. Zymo products are more reliable and I never used any software until now so I can’t say anything much about that. Overall, Zymo products are user friendly, more reliable and worth spending money on than any other kits. Also, online chat technical support is more useful at timely situations. I depend on Zymo more for epigenetics work and epigenetics applications. Zymo is the only company which can reach the research standard."
Status:
ReviewerMember since: 2016
Organization: bluebird bio
Very easy and efficient kit!
Application Area:Bisulfite sequencing library preparation
"The kit is very easy to use, the quick guide protocol gives clear instructions, and so far our results have been high quality and consistent. "
Status:
ReviewerMember since: 2016
Organization: University of Illinois
Great product
Application Area:Bisulfite treatment for methylation sequencing
"The product is easy to use and the process does not take much time. The quality of the result product is satisfying"
The EZ DNA Methylation-Lightning™ Kit features rapid and reliable bisulfite treatment and conversion of DNA for methylation analysis.
Key to the fast workflow is the ready-to-use Lightning Conversion Reagent. No preparation is necessary, simply add this unique reagent to a DNA sample, wait about an hour, and let the reaction proceed to completion. DNA denaturation and bisulfite conversion processes are combined with added heat to facilitate rapid denaturation. Desulphonation and clean-up of the converted DNA is performed using a unique low-elution spin column. High yield, converted DNA is ideal for PCR, array, bisulfite and next generation sequencing, etc.