EZ DNA Methylation-Lightning™ Kit by Zymo Research

Manufacturer Zymo Research  |   Model: D5030
4.8
/
5.0
  |  18 reviews
The EZ DNA Methylation-Lightning™ Kit features the fastest method for complete bisulfite conversion of DNA for methylation analysis.


EZ DNA Methylation-Lightning™ Kit by Zymo Research product image
EZ DNA Methylation-Lightning Bisulfite Conversion Kit
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Average Rating: 4.8
18 Scientists have reviewed this product

5 out of 5
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money


  • Status:

    Reviewer
  • Member since: 2021

  • Organization: INSTITUTO MAIMONIDES INVESTIGACION BIOMEDICA CORDOBA



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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More effective in less time.
Rating: 4.0

  • Application Area: Bisulfite conversion for DNA methylation analysis

"Quick, easy and effective bisulfite modification system. This improved version that considerably saves incubation time, is a great success for lovers of epigenetics."

Review date: 17 Mar 2021 | EZ DNA Methylation-Lightning™ Kit
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: Johns Hopkins Medicine



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
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Reliable products with consistent results.
Rating: 4.3

  • Application Area:Bisulfite conversion of human DNA

"EZ DNA Methylation-Lightning™ Kit is the bisulfite conversion kit we have been using for years. It appeared that this is one of the kits producing pure converted DNA and would not affect downstream applications such as digital PCR which requires high purity of DNA. It is a bead-based method, which is much easier to use than others. We use it for different starting concentrations from 100 pg to 2000 ng. We get consistent results from this kit all the time."

Review date: 16 Mar 2021 | EZ DNA Methylation-Lightning™ Kit
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: Johns Hopkins University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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High quality methylation results.
Rating: 5.0

  • Application Area:Whole genome sequencing data

"We experience very high-quality results with this product and find the results reproducible and very reliable. We have been using Zymo methylation kits for years now, first the gold kit and now we are mainly using the lighting kit. Tech support is always very helpful and responsive whenever we have questions."

Review date: 16 Mar 2021 | EZ DNA Methylation-Lightning™ Kit
  • Status:

    Reviewer

  • Member since: 2020

  • Organization: CellMax Life



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Easy to use bisulphite conversion kit
Rating: 5.0

  • Application Area:Methylation

"The lightning kit is super-easy to use, efficient and reliable resource for bisulphite conversion. Great tool for methylation research."

Review date: 17 Dec 2020 | EZ DNA Methylation-Lightning™ Kit
  • Status:

    Reviewer

  • Member since: 2020

  • Organization: Weizmann Institute of Science



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great results, easy to use.
Rating: 5.0

  • Application Area:Study DNA methylation during early development

"Easy to use, easy to scale down to single cell platforms and automation. Great results!"

Review date: 05 Dec 2020 | EZ DNA Methylation-Lightning™ Kit
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: TruMe Labs



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
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Very easy to use with great results.
Rating: 4.7

  • Application Area:Epigenetics

"EZ DNA Methylation-Lightning Kit is friendly to user product and provides rapid and full DNA conversion for epigenetic applications. The conversion is done in ~1h; and in our hands the quality of conversion is the same as for Gold kit. Converted DNA is then easily purified and is ready for subsequent PCR. Contrary to other conversion kits (e.g. Gold or Direct), Lighting reagent is ready-to-use and does not require solubilization of the reagent in different buffers."

Review date: 04 Dec 2020 | EZ DNA Methylation-Lightning™ Kit
  • Status:

    Reviewer

  • Member since: 2020

  • Organization: Johns Hopkins University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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"Go-to" Bisulfite conversion kit.
Rating: 5.0

  • Application Area:Bisulfite conversion

"This is our "go-to" kit for bisulfite. Fast, efficient and inexpensive. Seems to work as well or better than all other kits we've tried."

Review date: 17 Jul 2020 | EZ DNA Methylation-Lightning™ Kit
  • Status:

    Reviewer

  • Member since: 2020

  • Organization: University of Louisiana Monroe



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
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Great product and effective.
Rating: 4.7

  • Application Area:Methylation analysis in prostate cancer cells

"It’s easy to use but the only problem is it produces less product, requiring me to use more columns."

Review date: 05 Jun 2020 | EZ DNA Methylation-Lightning™ Kit
  • Status:

    Advanced Reviewer

  • Member since: 2019

  • Organization: Keck of USC



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Amazing product!
Rating: 5.0

  • Application Area:Analyze DNA methylation in cancer cells

"I have been using this kit for several years. All the reagents are included in the kit and ready-to use, which means the whole process can be done in two hours. The input DNA can go down to 100 pg, which is really important if my tissue samples are very limited. The output DNA is suitable for illumina DNA methylation array or next generation sequencing. The results are always reliable and reproducible."

Review date: 24 Jan 2020 | EZ DNA Methylation-Lightning™ Kit
  • Status:

    Advanced Reviewer

  • Member since: 2019

  • Organization: Keck of USC



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great kit for DNA methylation analysis
Rating: 5.0

  • Application Area:Analyze DNA methylation

"This kit is the best one I have used for DNA bisulfite conversion. It is easy and quick. The product quality is good and reproducible. I used the product for EPIC methylation array, sequencing, and COBRA assay - the results are great."

Review date: 11 Dec 2019 | EZ DNA Methylation-Lightning™ Kit


  • Status:

    Reviewer

  • Member since: 2009

  • Organization: UNIVERSITY OF OKLAHOMA HSC



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Best kit for methylation and nothing can over take this kit
Rating: 5.0

  • Application Area:Analyze methylation pattern in cancer cell lines with response to IR

"EZ DNA methylation lightning kit is the easiest kit for the bisulfite conversion and the methylation analysis. It gives a good yield of bisulfite converted DNA and the time required for this is very less."

Review date: 24 Oct 2017 | EZ DNA Methylation-Lightning™ Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: IRCM



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Greats results for NGS.
Rating: 5.0

  • Application Area:Methylation analysis

"High quality and reproducible results on next generation sequencing samples. Easy to use and high recovery efficiency. "

Review date: 29 Jul 2016 | EZ DNA Methylation-Lightning™ Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Kyung hee university



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Good results. High reproducibility. But low yield
Rating: 5.0

  • Application Area:Analyze methylation specific PCR

"High reproducibility but low yield. In protocol, it's possible to convert 2ug of DNA but for high effectiveness, they recommend 500ng, which is not enough to check for one time MSP for the first time. "

Review date: 28 Jul 2016 | EZ DNA Methylation-Lightning™ Kit
  • Status:

    Advanced Reviewer

  • Member since: 2016

  • Organization: InStem - National Centre for Biological Science



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
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A must buy for beginner.
Rating: 4.7

  • Application Area:Bisulfite conversion of DNA

"The only product which has given satisfactory yield of 100% converted DNA. PCR worked in first shot."

Review date: 28 Jul 2016 | EZ DNA Methylation-Lightning™ Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Facultad de Medicina, Univesidad de la Republica Oriental del Uruguay



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Excellent
Rating: 5.0

  • Application Area:Analyze methylation in blood samples

"The product covers our laboratory's every need, in an easy and cost-effective manner. It's really simple to use and you can always rely on customer service for help"

Review date: 25 Jul 2016 | EZ DNA Methylation-Lightning™ Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: The University of Oklahoma Health Sciences Center



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Zymo kit is the top one in epigenetics research.
Rating: 5.0

  • Application Area:Cancer research and methylation status.

"Nothing can replace Zymo products and the accuracy that I am getting from using this kit. Cost wise and work time wise, Zymo is user friendly. Also if we face any issues, Zymo technical support and the complete manual with the troubleshooting help us to figure out the solution. When compared with some top brand company kits, Zymo never loses its own standard in methylation and other molecular research. Due to its uniqueness and result accuracy, it's always good to stand with Zymo products. I am even an active user of some more of their products like Direct-zol™ RNA MiniPrep and DNA Clean & Concentrator™, which are always excellent products for research use. In epigenetics, ZYMO IS THE BEST AND WILL BE THE BEST, which you can trust blindly. I always strongly recommend to my colleagues to go for Zymo products for epigenetics work. Zymo always holds its name "The Epigenetics Company". I have no negative comments or negative reviews for Zymo products. Until now, I have never contacted technical support of Zymo because I have never faced any big issue in working with Zymo kits. MiniPrep kit is extremely easy to use and time saving. Compared to other products it is cheaper and less time consuming. I have also had very good results isolating different RNA sizes compared to conventional methods with trizol and, importantly, I always got the best purity. The microelution was key here. Zymo products are more reliable and I never used any software until now so I can’t say anything much about that. Overall, Zymo products are user friendly, more reliable and worth spending money on than any other kits. Also, online chat technical support is more useful at timely situations. I depend on Zymo more for epigenetics work and epigenetics applications. Zymo is the only company which can reach the research standard."

Review date: 22 Jul 2016 | EZ DNA Methylation-Lightning™ Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: bluebird bio



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
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Very easy and efficient kit!
Rating: 4.7

  • Application Area:Bisulfite sequencing library preparation

"The kit is very easy to use, the quick guide protocol gives clear instructions, and so far our results have been high quality and consistent. "

Review date: 21 Jul 2016 | EZ DNA Methylation-Lightning™ Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University of Illinois



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great product
Rating: 5.0

  • Application Area:Bisulfite treatment for methylation sequencing

"The product is easy to use and the process does not take much time. The quality of the result product is satisfying"

Review date: 13 Jul 2016 | EZ DNA Methylation-Lightning™ Kit

The EZ DNA Methylation-Lightning™ Kit features rapid and reliable bisulfite treatment and conversion of DNA for methylation analysis.

Key to the fast workflow is the ready-to-use Lightning Conversion Reagent. No preparation is necessary, simply add this unique reagent to a DNA sample, wait about an hour, and let the reaction proceed to completion. DNA denaturation and bisulfite conversion processes are combined with added heat to facilitate rapid denaturation. Desulphonation and clean-up of the converted DNA is performed using a unique low-elution spin column. High yield, converted DNA is ideal for PCR, array, bisulfite and next generation sequencing, etc.

Product Overview

EZ DNA Methylation-Lightning™ Kit by Zymo Research product image

EZ DNA Methylation-Lightning™ Kit

Manufacturer Zymo Research

4.8 / 5.0 | 18 reviews