EZ DNA Methylation™ Kit by Zymo Research

Manufacturer Zymo Research  |   Model: D5001
4.8
/
5.0
  |  13 reviews
The EZ DNA Methylation™ Kit is a streamlined, proven procedure for bisulfite conversion of DNA.


EZ DNA Methylation™ Kit by Zymo Research product image
EZ DNA Methylation Bisulfite Conversion Kit
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Average Rating: 4.8
13 Scientists have reviewed this product

5 out of 5
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money


  • Status:

    Reviewer
  • Member since: 2019

  • Organization: UCSF



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
The kit is easy to use! Well written manual.
Rating: 4.3

  • Application Area: Running methylation microarrays

"The Zymo Research's EZ DNA Methylation Kits (part D5002 and D5004) are relatively easy to use. The instruction manuals are detailed with a quick background of the methodology, a list of the consumables, reagent preparation, protocol, and optimization tips. The instructions weren't bogged down by extraneous details, but enough information for a quick overview and usage instructions. I started off using the spin-column kits of D5002, but I realized that when processing 32 samples at a time, using individual tubes was not optimal for my time. I would recommend this kit if you just have a few reactions to run at a time. I progressed to the 2x96 reaction kit of D5004. I was happy I could use the multichannel pipettes for streamlining my work process. I contacted Zymo's tech support for suggestions from using kits D5002 and D5004; they were very helpful in my transition between the two kits. In both cases, my methylated products produced robust and consistent results in my microarray experiments."

Review date: 27 Nov 2019 | EZ DNA Methylation™ Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: UCdenver



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
I would highly recommend after having used it for many years!
Rating: 5.0

  • Application Area:Used to methylate samples

"This product is very robust and easy to use for processing Illumina samples."

Review date: 31 Jul 2019 | EZ DNA Methylation™ Kit
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Primordia Lifesciences



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
A must for epigenetic studies.
Rating: 5.0

  • Application Area:DNA modifications in tissues

"EZ DNA methylation kit is very easy to use, gives consistent and fast results."

Review date: 05 Jun 2019 | EZ DNA Methylation™ Kit
  • Status:

    Reviewer

  • Member since: 2012

  • Organization: Columbia University



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
Used with confidence. Reproducibility in the results, easy to use, not time consuming.
Rating: 4.7

  • Application Area:Genomic DNA analysis

"I have used the EZ DNA Methylation kit for a methylation promoter analysis. The use of the product was simple and straightforward. Good reproducibility and not time consuming. I was confident in the results. I have done bisulfite conversion DNA treatment and I was surprised with the kit's simplified and streamlined procedure."

Review date: 05 Jun 2019 | EZ DNA Methylation™ Kit
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: ucdenver



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
Great and only product we use for methylation.
Rating: 4.3

  • Application Area:Methylate Illumina samples

"This is a robust and excellent product that produces exactly what we need every time."

Review date: 30 Jun 2017 | EZ DNA Methylation™ Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Duke University Medical Center



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Great results time after time
Rating: 5.0

  • Application Area:methylation anaylsis

"This is our preferred kit for DNA conversion. It's consistent, easy to use and affordable. We most of the time convert 800ng of DNA and elute in 40ul of buffer."

Review date: 18 Jan 2017 | EZ DNA Methylation™ Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University of California at Los Angeles



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Amazing results!! Highly recommended.
Rating: 4.7

  • Application Area:Quantify methylation in mouse tissues

"We have used this kit to quantify global methylation status in mouse lungs tissue. Very user friendly kit and reproducible results."

Review date: 29 Jul 2016 | EZ DNA Methylation™ Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: LUMC



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Great and reliable conversions.
Rating: 4.3

  • Application Area:DNA methylation measurements in genomic DNA

"The EZ DNA methylation kit, despite all the new iterations also available from Zymo, is still our go-to kit for large projects. Input DNA needs to be of high quality but if so the kit reliably gives full bisulfite conversions, and we have done thousands of conversions to date. Recommend. "

Review date: 29 Jul 2016 | EZ DNA Methylation™ Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: UNC Gillings School of Global Public Health



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Consistent results and easy to use - makes lab life easier!
Rating: 5.0

  • Application Area:Conversion prior to Illumina methylation assessment

"The EZ DNA Methylation Kit is easy to use and gives consistent results. Plus, DNA yields were the best of all kits tried. Zymo is our go-to methylation reagent company!"

Review date: 13 Jul 2016 | EZ DNA Methylation™ Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: UCdenver



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
awesome all around product
Rating: 5.0

  • Application Area:methylation for illumina 850K chip

"easy to use and produce robust results -great value for price"

Review date: 12 Jul 2016 | EZ DNA Methylation™ Kit


  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University of Cincinnati



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Standard and reliable kit for bisulfite conversion
Rating: 5.0

  • Application Area:DNA methylation analysis of blood and tissue

"This is the tried and true kit for bisulfite conversion of DNA. Consistent and reliable!"

Review date: 12 Jul 2016 | EZ DNA Methylation™ Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: UMR8199-CNRS



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Efficiency
Rating: 5.0

  • Application Area:Epigenetics

"This product is the best that I've seen for the bisulfite conversion. It's deadly efficient and robust."

Review date: 12 Jul 2016 | EZ DNA Methylation™ Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: NIMHANS



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
Great results. This is the first and foremost step we do. Thoroughly satisfied!
Rating: 4.7

  • Application Area:Blood samples

"It's an excellent product. We use it for converting our DNA extracted from blood samples to study epigenetics. The only tedious work is inverting the samples several times. Otherwise the product is very good in yielding high quality bisulfite converted products"

Review date: 12 Jul 2016 | EZ DNA Methylation™ Kit

The EZ DNA Methylation™ Kit features a simplified procedure that streamlines bisulfite treatment of DNA.

The kit is based on the three-step reaction that takes place between cytosine and sodium bisulfite where cytosine is converted into uracil. The product's innovative in-column desulphonation technologies eliminate otherwise cumbersome precipitations. The kit is designed to reduce template degradation, minimize DNA loss during treatment and cleanup, while ensuring complete conversion of the DNA. Purified, converted DNA is ideal for PCR amplification for downstream analyses including endonuclease digestion, sequencing, microarrays, etc. This kit is validated for use with Illumina's GoldenGate® and Infinium® Assays.

Product Overview

EZ DNA Methylation™ Kit by Zymo Research product image

EZ DNA Methylation™ Kit

Manufacturer Zymo Research

4.8 / 5.0 | 13 reviews