EZ DNA Methylation-Gold™ Kit by Zymo Research

Manufacturer Zymo Research  |   Model: D5005
4.6
/
5.0
  |  27 reviews
The EZ DNA Methylation-Gold™ Kit allows for complete bisulfite conversion of GC-rich DNA in less than 3 hours.


EZ DNA Methylation-Gold™ Kit by Zymo Research product image
EZ DNA Methylation-Gold Bisulfite Conversion Kit
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Average Rating: 4.6
27 Scientists have reviewed this product

5 out of 5
Ease of use
4 out of 5
After sales service
5 out of 5
Value for money


  • Status:

    Reviewer
  • Member since: 2021

  • Organization: Danforth



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
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Necessary for my research.
Rating: 4.7

  • Application Area: Epigenetics

"This kit is essential in my lab, we use it on a weekly basis. We have compared different kits for bisulfate conversion and to date is the best one available. Easy to use, user-friendly and quick protocol. "

Review date: 26 May 2021 | EZ DNA Methylation-Gold™ Kit
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: Danforth



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
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Indispensable reagent in my lab.
Rating: 4.7

  • Application Area:Epigenetics

"Zymo DNA methylation gold kit is very useful for my research. Reagents and instructions are user-friendly and easy to use. My lab uses it on a daily basis, and to today, it has been the best product in the market for DNA bisulfate conversion."

Review date: 20 May 2021 | EZ DNA Methylation-Gold™ Kit
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: universidad juarez autonoma de tabasco



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Good performance with high quality.
Rating: 5.0

  • Application Area:Evaluation of global DNA methylation via RT-PCR

"I tested different products for modification of genomic DNA, observing that the recovered amount after treatment and purification was too low. With the EZ DNA methylation-gold kit, I obtained high concentrations of modified DNA with its protocol. The high quality of the recovered material allow its analysis by real-time PCR using probes for quantitative analysis of global DNA methylation through ALU, LINE, and Sat sequences."

Review date: 16 Mar 2021 | EZ DNA Methylation-Gold™ Kit
  • Status:

    Reviewer

  • Member since: 2020

  • Organization: Johns Hopkins University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Current best conversion kit for samples with low DNA.
Rating: 5.0

  • Application Area:Single-cell bisulfite sequencing

"We tested multiple bisulfite conversion kits in our scBS-Seq project and found that the EZ DNA Methylation-Gold Kit resulted in improved converted DNA yields and integrity. Highly recommend for converting DNA for samples with limited amounts of DNA."

Review date: 04 Dec 2020 | EZ DNA Methylation-Gold™ Kit
  • Status:

    Reviewer

  • Member since: 2020

  • Organization: Johns Hopkins School of Medicine



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great results, definitely recommend this product.
Rating: 5.0

  • Application Area:Digital PCR/epigentic biomarker discovery & assay development

"EZ DNA Methylation-Gold kit is the default bisulfite conversion kit in our lab. It is easy to use and gives a pretty high DNA recovery rate. Compared to the 96-well plate method, the column-based method is less-troublesome and more user friendly."

Review date: 15 Aug 2020 | EZ DNA Methylation-Gold™ Kit
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: CERVIVA



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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Good kit and reliable.
Rating: 4.0

  • Application Area:Bisulfite conversion of cervical specimens

"Overall a relatively simple and straight forward process. Care must be taken to reduce cross contamination but found the product to be effective as well as reliable in over 1000 uses. The one or two times we contacted customer care, they were good and helpful. Would use again in future work if required."

Review date: 28 Nov 2019 | EZ DNA Methylation-Gold™ Kit
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Navarrabiomed



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Easy-to-use kit which provides reproducible results.
Rating: 5.0

  • Application Area:Bisulphite conversion of DNA to analyze DNA methylation

"It is a very easy-to-use kit to bisulphite-transform DNA. We have used it routinely for years with very reproducible and high-quality results, regardless of the sample source (human cell lines and frozen or formalin-fixed paraffin-embedded tissues). I like least that there is a very long incubation step in the protocol. Potential users should know that the after sales care has been rated with 5 stars, but we have never had any problem with the kit, and never needed to contact them. The 5-star rating is based on this."

Review date: 27 Nov 2019 | EZ DNA Methylation-Gold™ Kit
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: NCSU



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Reliable product.
Rating: 5.0

  • Application Area:Synthetic biology

"The product is easy to use. Results from pyrosequencing are of high quality and reproducible."

Review date: 05 Jun 2019 | EZ DNA Methylation-Gold™ Kit
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Lady Davis Institute



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
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I can swear by it.
Rating: 4.7

  • Application Area:Analyzing DNA methylation in liver samples

"This product was effective in converting the DNA to bisulfite form. I absolutely loved the post-purchase customer care. The rep was very responsive and helped me with the downstream process. I was impressed."

Review date: 05 Jun 2019 | EZ DNA Methylation-Gold™ Kit
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: UCLA



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Good quality product.
Rating: 5.0

  • Application Area:Methylation of gDNA

"Easy to use and good quality product achieved. However some improvements are required, like the quality of collection plates and the mixing of samples with binding buffer in filter plates is not RSI friendly."

Review date: 05 Jun 2019 | EZ DNA Methylation-Gold™ Kit


  • Status:

    Advanced Reviewer

  • Member since: 2019

  • Organization: Drexel University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    3 out of 5
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Super easy kit to use. I recommend this kit to all of my colleagues.
Rating: 4.3

  • Application Area:Epigenetics

"Super easy kit to use. I've never had to troubleshoot this kit. I recommend this kit to all of my colleagues."

Review date: 05 Jun 2019 | EZ DNA Methylation-Gold™ Kit
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Drexel University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
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Always works great!
Rating: 4.7

  • Application Area:DNA methylation

"Great results every time, protocol easy to follow and never had to troubleshoot."

Review date: 05 Jun 2019 | EZ DNA Methylation-Gold™ Kit
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: cincinnati children hospital



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great result.
Rating: 5.0

  • Application Area:Human sample

"The conversion rate is great, it is easy to use and yield is much higher than other products on the market."

Review date: 05 Jun 2019 | EZ DNA Methylation-Gold™ Kit
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Wake Forest Baptist Medical Health



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Easy to use and fast kit for bisulfite conversion.
Rating: 5.0

  • Application Area:Analysis of circulating DNA methylation

"Easy and fast kit to use for the bisulfite conversion, with high conversion efficiency, suitable for massive parallel sequencing."

Review date: 05 Jun 2019 | EZ DNA Methylation-Gold™ Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University of Manitoba



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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Very good results
Rating: 4.0

  • Application Area:Methylation

"This kit is very efficient and fast for bisulfite conversation."

Review date: 29 Jul 2016 | EZ DNA Methylation-Gold™ Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University of Athens



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    4 out of 5
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Good results, value for money
Rating: 4.0

  • Application Area:DNA methylation studies

"High quality of SB treated DNA, good reproducibility and recovery. Suitable for downstream application e.g. Real Time MSP"

Review date: 29 Jul 2016 | EZ DNA Methylation-Gold™ Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Gothenburg University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Overall good results..I recommend this product
Rating: 5.0

  • Application Area:Epigenetics

"I regularly use this product and I had known that there are other types, but I am succeeding every time with reproducible results within short times so I prefer to use this than other companies’ ones. The gold kit takes little time to convert bisulphite converted DNA than other ones. I am happy with this product. Thank you Zymo for the nice product..! Keep update me if you have better modified product of this in future. Pradeep "

Review date: 29 Jul 2016 | EZ DNA Methylation-Gold™ Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Institute of Cancer Research



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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Essential part of our methylation study workflow.
Rating: 4.0

  • Application Area:Analysis of methylation status of ctDNA

"Very easy to use. Should be the gold standard for methylation studies."

Review date: 29 Jul 2016 | EZ DNA Methylation-Gold™ Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University of Patras



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
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High efficiency in bisulfite conversion.
Rating: 4.7

  • Application Area:Methylation in cancer tissues

"EZ DNA Methylation-Gold™ Kit is very easy to use, the procedure is shorter compared to other manufacturers and has high efficiency in bisulfite conversion, even in difficult samples, like cell free circulating DNA in plasma and paraffin embedded tissues."

Review date: 28 Jul 2016 | EZ DNA Methylation-Gold™ Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Faculty of Science, University of Zagreb



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Good value for money.
Rating: 5.0

  • Application Area:Analysis of DNA methylation

"The kit is easy to use and reliable. The protocol doesn't take long (approximately an hour of hands-on time)."

Review date: 28 Jul 2016 | EZ DNA Methylation-Gold™ Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: IIT MADRAS



  • Ease of use
    4 out of 5
    After sales service
    3 out of 5
    Value for money
    4 out of 5
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Very easy and reliable kits blindly u can do your experiments.
Rating: 3.7

  • Application Area:Stem cell epigenomics

"I have used Zymo products of these I like DNA methylation kit and PCR product gel clean up kit. Both are extremely good. It is cheap and most importantly the quality of the product is up to the mark."

Review date: 28 Jul 2016 | EZ DNA Methylation-Gold™ Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: James Madison University



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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Easy & effective BS mutagenesis.
Rating: 4.3

  • Application Area:Epigenetics

"Easy to use and reliable bisulfite mutagenesis kit. My undergraduate students are routinely able to get quality conversion of 50-500 ng of genomic DNA BS PCR."

Review date: 28 Jul 2016 | EZ DNA Methylation-Gold™ Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: UBC



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great kit for bisulfite treatment!
Rating: 5.0

  • Application Area:For bisulfite treatment of samples

"Achieve very reproducible results. The bisulfite conversion in this kit is highly efficient, with over 98% of molecules fully converted."

Review date: 27 Jul 2016 | EZ DNA Methylation-Gold™ Kit
  • Status:

    Reviewer

  • Member since: 2015

  • Organization: Affiliated Genetics



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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Very user friendly. Would Recommend.
Rating: 4.3

  • Application Area:Bisulfite Conversion

"Zymo Research's EZ DNA Methylation Gold Kit is incredibly easy to use. In the kit comes the very short and easy protocol along with each reagent necessary for the process. All things are included in the kit. The results were just about where they said they would be. The conversions factor’s right on."

Review date: 21 Jul 2016 | EZ DNA Methylation-Gold™ Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Augusta University



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
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It is a great kit.
Rating: 4.7

  • Application Area:Pyrosequencing

"It is easy to use and I have always been getting consistent results."

Review date: 21 Jul 2016 | EZ DNA Methylation-Gold™ Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Universidad de Cordoba



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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The results are good enough, but the efficiency rate could be improved.
Rating: 4.3

  • Application Area:Analyses of DNA methylation

"The kit is efficient enough and it worked well for me. Sometimes the efficiency is above the threshold (I mean, the controls were not totally converted), but this happened few times (15-20%). I think this could be improved but it is so easy to use that I still considering the best option. "

Review date: 13 Jul 2016 | EZ DNA Methylation-Gold™ Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Genome Quebec



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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very good product for methylation analysis
Rating: 5.0

  • Application Area:service provider

"clean and constant product, very good support for developing testing "

Review date: 12 Jul 2016 | EZ DNA Methylation-Gold™ Kit

The EZ DNA Methylation-Gold™ Kit is a refinement of our popular EZ DNA Methylation™ kits.

These products consolidate DNA denaturation and bisulfite conversion processes into one step, leading to a much faster bisulfite conversion. This is accomplished using temperature denaturation to complement chemical denaturation in the previous protocol. Also, this kit have been streamlined for high yield recovery of DNA following bisulfite treatment. Recovered bisulfite-converted DNA is ideal for PCR amplification for downstream analyses including endonuclease digestion, sequencing, microarrays, etc.

Product Overview

EZ DNA Methylation-Gold™ Kit by Zymo Research product image

EZ DNA Methylation-Gold™ Kit

Manufacturer Zymo Research

4.6 / 5.0 | 27 reviews