EZ DNA Methylation-Gold™ Kit by Zymo Research

"This is great kit. Since bisulfite conversion is a very delicate process, this kit provided me with high quality bisulfite DNA, suitable for methylation analysis with pyrosequencing."

"This kit made the preparation for pyrosequencing easy and straightforward. The instructions are simple and easy to learn when I first started using this kit."

"EZ DNA Methylation-Gold kit is the only bisulfite modification kit that we have been using for many years in our lab. We get reproducible results and high DNA yield. It is very easy to use. "

"Dollar for dollar, this kit is of exceptional value!"

"The EZ DNA Methylation-Gold Kit is really easy to use and very efficient. Robust and reproductive conversion. The after-sales care is good too, not really necessary since most of the troubleshooting and optimizations are write down in the manual. The ratio quality / price is pretty good, I recommend this product."

"This kit is essential in my lab, we use it on a weekly basis. We have compared different kits for bisulfate conversion and to date is the best one available. Easy to use, user-friendly and quick protocol. "

"Zymo DNA methylation gold kit is very useful for my research. Reagents and instructions are user-friendly and easy to use. My lab uses it on a daily basis, and to today, it has been the best product in the market for DNA bisulfate conversion."

"I tested different products for modification of genomic DNA, observing that the recovered amount after treatment and purification was too low. With the EZ DNA methylation-gold kit, I obtained high concentrations of modified DNA with its protocol. The high quality of the recovered material allow its analysis by real-time PCR using probes for quantitative analysis of global DNA methylation through ALU, LINE, and Sat sequences."

"We tested multiple bisulfite conversion kits in our scBS-Seq project and found that the EZ DNA Methylation-Gold Kit resulted in improved converted DNA yields and integrity. Highly recommend for converting DNA for samples with limited amounts of DNA."

"EZ DNA Methylation-Gold kit is the default bisulfite conversion kit in our lab. It is easy to use and gives a pretty high DNA recovery rate. Compared to the 96-well plate method, the column-based method is less-troublesome and more user friendly."

"Overall a relatively simple and straight forward process. Care must be taken to reduce cross contamination but found the product to be effective as well as reliable in over 1000 uses. The one or two times we contacted customer care, they were good and helpful. Would use again in future work if required."

"It is a very easy-to-use kit to bisulphite-transform DNA. We have used it routinely for years with very reproducible and high-quality results, regardless of the sample source (human cell lines and frozen or formalin-fixed paraffin-embedded tissues). I like least that there is a very long incubation step in the protocol. Potential users should know that the after sales care has been rated with 5 stars, but we have never had any problem with the kit, and never needed to contact them. The 5-star rating is based on this."

"The product is easy to use. Results from pyrosequencing are of high quality and reproducible."

"This product was effective in converting the DNA to bisulfite form. I absolutely loved the post-purchase customer care. The rep was very responsive and helped me with the downstream process. I was impressed."

"Easy to use and good quality product achieved. However some improvements are required, like the quality of collection plates and the mixing of samples with binding buffer in filter plates is not RSI friendly."

"Super easy kit to use. I've never had to troubleshoot this kit. I recommend this kit to all of my colleagues."

"Great results every time, protocol easy to follow and never had to troubleshoot."

"The conversion rate is great, it is easy to use and yield is much higher than other products on the market."

"Easy and fast kit to use for the bisulfite conversion, with high conversion efficiency, suitable for massive parallel sequencing."

"This kit is very efficient and fast for bisulfite conversation."

"High quality of SB treated DNA, good reproducibility and recovery. Suitable for downstream application e.g. Real Time MSP"

"I regularly use this product and I had known that there are other types, but I am succeeding every time with reproducible results within short times so I prefer to use this than other companies’ ones. The gold kit takes little time to convert bisulphite converted DNA than other ones. I am happy with this product. Thank you Zymo for the nice product..! Keep update me if you have better modified product of this in future. Pradeep "

"Very easy to use. Should be the gold standard for methylation studies."

"EZ DNA Methylation-Gold™ Kit is very easy to use, the procedure is shorter compared to other manufacturers and has high efficiency in bisulfite conversion, even in difficult samples, like cell free circulating DNA in plasma and paraffin embedded tissues."

"The kit is easy to use and reliable. The protocol doesn't take long (approximately an hour of hands-on time)."

"I have used Zymo products of these I like DNA methylation kit and PCR product gel clean up kit. Both are extremely good. It is cheap and most importantly the quality of the product is up to the mark."

"Easy to use and reliable bisulfite mutagenesis kit. My undergraduate students are routinely able to get quality conversion of 50-500 ng of genomic DNA BS PCR."

"Achieve very reproducible results. The bisulfite conversion in this kit is highly efficient, with over 98% of molecules fully converted."

"Zymo Research's EZ DNA Methylation Gold Kit is incredibly easy to use. In the kit comes the very short and easy protocol along with each reagent necessary for the process. All things are included in the kit. The results were just about where they said they would be. The conversions factor’s right on."

"It is easy to use and I have always been getting consistent results."

"The kit is efficient enough and it worked well for me. Sometimes the efficiency is above the threshold (I mean, the controls were not totally converted), but this happened few times (15-20%). I think this could be improved but it is so easy to use that I still considering the best option. "

"clean and constant product, very good support for developing testing "