Status:
ReviewerMember since: 2022
Organization: Central Molecular Lab, GIPMER, New Delhi
Bio-magical boxes that are robust at work, smooth to use, and easy on pockets.
Application Area: To study cancer epigenetics
"Biomolecular research and technology are growing at a tremendous pace. The services of scientific manufacturers are undoubtedly at the frontline of maintaining the smoothness and integrity of our experimentations. In molecular biology where every protocol can be laborious and time-consuming, Zymo Research like Santa Claus brings its products/gifts of happiness to our lab. All of my lab mates are used to or can say addicted to some Zymo products and I personally am excited to review Zymo's EZ DNA Methylation-Gold Kit. I remember that when I put the study to explore the methylation changes of some tumor suppressors and oncogenes in the CRC tissue samples as one of my research objectives, I was not sure that with the support from Zymo, it could be this much easy and satisfactory. Moreover, since my project has funding constraints yet with Zymo I have saved enough to add a new objective to my current study. I cannot thank Zymo Research enough for providing these "bio-magical boxes" that are not only simplifying science but making it worth every penny we provide."
Status:
ReviewerMember since: 2021
Organization: University of Minnesota Medical School
Gold standard!
Application Area:Analyze methylation changes in chronic pain
"Great product, always able to get bisulfite converted DNA."
Status:
ReviewerMember since: 2022
Organization: University of Teramo
Good performance with high reproducibility
Application Area:DNA methylation at specific genomic regions.
"My lab uses EZ DNA Methylation-Gold™ Kit on a daily basis to generate a bisulfite-converted genome suitable for the study of DNA methylation at specific genomic regions. I always had great results with this kit using DNA from cells as well as tissue and saliva. Its protocol is clear, faster and is easy to follow. The high quality of the recovered converted DNA allow its analysis by pyrosequencing and MSP-PCR. The ratio quality / price is pretty good, I consider it as an indispensable reagent for my research."
Status:
ReviewerMember since: 2022
Organization: Istanbul University
Had great results with this kit.
Application Area:DNA bisulfite conversion
"Worked without any problems during bisulfite conversion for our methylation studies."
Status:
Advanced ReviewerMember since: 2019
Organization: University of Wolverhampton
Great product
Application Area:Cancer Research
"This kit has a very straightforward protocol that is clear and easy to follow that are easily reproducible in cancer cell lines looking for the presence of any methylation present."
Status:
ReviewerMember since: 2022
Organization: Tufts University
Great product for a good price.
Application Area:Bisulfite conversion of DNA from breast cancer cell lines
"Easy to use, high-quality DNA converted, and can be used with a small number of cells. High concentration of DNA converted recovery."
Status:
ReviewerMember since: 2022
Organization: Washington University in St. Louis
Great, reliable protocol
Application Area:WGBS of lung cancer cell line
"The protocol is relatively straightforward and I obtained robust C to T conversion"
Status:
Advanced ReviewerMember since: 2021
Organization: St jude childrens research hospital
Fast and easy to use
Application Area:Very easy to use and robust conversion of g.DNA
"I used this kit to generate a bisulfite-converted genome. The protocol is very simple, and faster and the amount of recovered converted DNA is almost 80% of the input DNA."
Status:
ReviewerMember since: 2022
Organization: PROMIS DIAGNOSTICS
Methylation study is easy!
Application Area:Research of DNA methylation for cancer diagnosis
"The kits is easy to use even for the novice in methylation research field. The consistency between the repeat samples was very great and the accuracy is also very high. When I have question related to the kit, the technical support team and sales rep quickly respond and solve the questions."
Status:
ReviewerMember since: 2022
Organization: Center for Functional Genomics
Best methyl-seq data ever!
Application Area:Epigenetics
"This product was used for a triple negative breast cancer cell line study evaluating the efficacy of various drug treatments including JQ1 and TSA. The best aspect of this kit is it worked the first time right out of the box. Following the manufacturer's protocol directly the kit yielded approximately 150 ng of bisulfite treated DNA for direct input to Accel-NGS methylseq DNA library preparation. All libraries looked uniform by Bioanalyzer and Qubit/qPCR quantification. Data from this study showed clear distinctions between treatments and untreated samples. The product was extremely easy to use. The supplied protocol was thorough, precise and correct. The results were highly reproducible and exceptional. The value for money couldn't be beat!"
Status:
ReviewerMember since: 2021
Organization: LUMC
Best methylation kit available!
Application Area:Pyrosequencing
"This is great kit. Since bisulfite conversion is a very delicate process, this kit provided me with high quality bisulfite DNA, suitable for methylation analysis with pyrosequencing."
Status:
ReviewerMember since: 2021
Organization: University of California, Davis
Quality kit, easy instructions.
Application Area:Analyze DNA methylation in cell culture samples
"This kit made the preparation for pyrosequencing easy and straightforward. The instructions are simple and easy to learn when I first started using this kit."
Status:
Advanced ReviewerMember since: 2016
Organization: McGill Univ
We highly recommend it to those who need to bisulfite-modify DNA samples.
Application Area:DNA methylation analysis
"EZ DNA Methylation-Gold kit is the only bisulfite modification kit that we have been using for many years in our lab. We get reproducible results and high DNA yield. It is very easy to use. "
Status:
ReviewerMember since: 2021
Organization: Columbia Universtity
Great efficienty and reproducibility.
Application Area:DNA Methylation
"The EZ DNA Methylation-Gold Kit is really easy to use and very efficient. Robust and reproductive conversion. The after-sales care is good too, not really necessary since most of the troubleshooting and optimizations are write down in the manual. The ratio quality / price is pretty good, I recommend this product."
Status:
ReviewerMember since: 2021
Organization: Danforth
Necessary for my research.
Application Area:Epigenetics
"This kit is essential in my lab, we use it on a weekly basis. We have compared different kits for bisulfate conversion and to date is the best one available. Easy to use, user-friendly and quick protocol. "
Status:
ReviewerMember since: 2021
Organization: Danforth
Indispensable reagent in my lab.
Application Area:Epigenetics
"Zymo DNA methylation gold kit is very useful for my research. Reagents and instructions are user-friendly and easy to use. My lab uses it on a daily basis, and to today, it has been the best product in the market for DNA bisulfate conversion."
Status:
ReviewerMember since: 2021
Organization: NULL
Good performance with high quality.
Application Area:Evaluation of global DNA methylation via RT-PCR
"I tested different products for modification of genomic DNA, observing that the recovered amount after treatment and purification was too low. With the EZ DNA methylation-gold kit, I obtained high concentrations of modified DNA with its protocol. The high quality of the recovered material allow its analysis by real-time PCR using probes for quantitative analysis of global DNA methylation through ALU, LINE, and Sat sequences."
Status:
ReviewerMember since: 2020
Organization: Johns Hopkins University
Current best conversion kit for samples with low DNA.
Application Area:Single-cell bisulfite sequencing
"We tested multiple bisulfite conversion kits in our scBS-Seq project and found that the EZ DNA Methylation-Gold Kit resulted in improved converted DNA yields and integrity. Highly recommend for converting DNA for samples with limited amounts of DNA."
Status:
ReviewerMember since: 2020
Organization: Johns Hopkins School of Medicine
Great results, definitely recommend this product.
Application Area:Digital PCR/epigentic biomarker discovery & assay development
"EZ DNA Methylation-Gold kit is the default bisulfite conversion kit in our lab. It is easy to use and gives a pretty high DNA recovery rate. Compared to the 96-well plate method, the column-based method is less-troublesome and more user friendly."
Status:
ReviewerMember since: 2019
Organization: CERVIVA
Good kit and reliable.
Application Area:Bisulfite conversion of cervical specimens
"Overall a relatively simple and straight forward process. Care must be taken to reduce cross contamination but found the product to be effective as well as reliable in over 1000 uses. The one or two times we contacted customer care, they were good and helpful. Would use again in future work if required."
Status:
ReviewerMember since: 2019
Organization: Navarrabiomed
Easy-to-use kit which provides reproducible results.
Application Area:Bisulphite conversion of DNA to analyze DNA methylation
"It is a very easy-to-use kit to bisulphite-transform DNA. We have used it routinely for years with very reproducible and high-quality results, regardless of the sample source (human cell lines and frozen or formalin-fixed paraffin-embedded tissues). I like least that there is a very long incubation step in the protocol. Potential users should know that the after sales care has been rated with 5 stars, but we have never had any problem with the kit, and never needed to contact them. The 5-star rating is based on this."
Status:
ReviewerMember since: 2019
Organization: NCSU
Reliable product.
Application Area:Synthetic biology
"The product is easy to use. Results from pyrosequencing are of high quality and reproducible."
Status:
ReviewerMember since: 2019
Organization: Lady Davis Institute
I can swear by it.
Application Area:Analyzing DNA methylation in liver samples
"This product was effective in converting the DNA to bisulfite form. I absolutely loved the post-purchase customer care. The rep was very responsive and helped me with the downstream process. I was impressed."
Status:
ReviewerMember since: 2019
Organization: UCLA
Good quality product.
Application Area:Methylation of gDNA
"Easy to use and good quality product achieved. However some improvements are required, like the quality of collection plates and the mixing of samples with binding buffer in filter plates is not RSI friendly."
Status:
Advanced ReviewerMember since: 2019
Organization: Drexel University
Super easy kit to use. I recommend this kit to all of my colleagues.
Application Area:Epigenetics
"Super easy kit to use. I've never had to troubleshoot this kit. I recommend this kit to all of my colleagues."
Status:
ReviewerMember since: 2019
Organization: Drexel University
Always works great!
Application Area:DNA methylation
"Great results every time, protocol easy to follow and never had to troubleshoot."
Status:
ReviewerMember since: 2019
Organization: cincinnati children hospital
Great result.
Application Area:Human sample
"The conversion rate is great, it is easy to use and yield is much higher than other products on the market."
Status:
ReviewerMember since: 2019
Organization: Wake Forest Baptist Medical Health
Easy to use and fast kit for bisulfite conversion.
Application Area:Analysis of circulating DNA methylation
"Easy and fast kit to use for the bisulfite conversion, with high conversion efficiency, suitable for massive parallel sequencing."
Status:
ReviewerMember since: 2016
Organization: University of Manitoba
Very good results
Application Area:Methylation
"This kit is very efficient and fast for bisulfite conversation."
Status:
ReviewerMember since: 2016
Organization: University of Athens
Good results, value for money
Application Area:DNA methylation studies
"High quality of SB treated DNA, good reproducibility and recovery. Suitable for downstream application e.g. Real Time MSP"
Status:
ReviewerMember since: 2016
Organization: Gothenburg University
Overall good results..I recommend this product
Application Area:Epigenetics
"I regularly use this product and I had known that there are other types, but I am succeeding every time with reproducible results within short times so I prefer to use this than other companies’ ones. The gold kit takes little time to convert bisulphite converted DNA than other ones. I am happy with this product. Thank you Zymo for the nice product..! Keep update me if you have better modified product of this in future. Pradeep "
Status:
ReviewerMember since: 2016
Organization: Institute of Cancer Research
Essential part of our methylation study workflow.
Application Area:Analysis of methylation status of ctDNA
"Very easy to use. Should be the gold standard for methylation studies."
Status:
ReviewerMember since: 2016
Organization: University of Patras
High efficiency in bisulfite conversion.
Application Area:Methylation in cancer tissues
"EZ DNA Methylation-Gold™ Kit is very easy to use, the procedure is shorter compared to other manufacturers and has high efficiency in bisulfite conversion, even in difficult samples, like cell free circulating DNA in plasma and paraffin embedded tissues."
Status:
ReviewerMember since: 2016
Organization: Faculty of Science, University of Zagreb
Good value for money.
Application Area:Analysis of DNA methylation
"The kit is easy to use and reliable. The protocol doesn't take long (approximately an hour of hands-on time)."
Status:
ReviewerMember since: 2016
Organization: IIT MADRAS
Very easy and reliable kits blindly u can do your experiments.
Application Area:Stem cell epigenomics
"I have used Zymo products of these I like DNA methylation kit and PCR product gel clean up kit. Both are extremely good. It is cheap and most importantly the quality of the product is up to the mark."
Status:
ReviewerMember since: 2016
Organization: James Madison University
Easy & effective BS mutagenesis.
Application Area:Epigenetics
"Easy to use and reliable bisulfite mutagenesis kit. My undergraduate students are routinely able to get quality conversion of 50-500 ng of genomic DNA BS PCR."
Status:
ReviewerMember since: 2016
Organization: UBC
Great kit for bisulfite treatment!
Application Area:For bisulfite treatment of samples
"Achieve very reproducible results. The bisulfite conversion in this kit is highly efficient, with over 98% of molecules fully converted."
Status:
ReviewerMember since: 2015
Organization: Affiliated Genetics
Very user friendly. Would Recommend.
Application Area:Bisulfite Conversion
"Zymo Research's EZ DNA Methylation Gold Kit is incredibly easy to use. In the kit comes the very short and easy protocol along with each reagent necessary for the process. All things are included in the kit. The results were just about where they said they would be. The conversions factor’s right on."
Status:
ReviewerMember since: 2016
Organization: Augusta University
It is a great kit.
Application Area:Pyrosequencing
"It is easy to use and I have always been getting consistent results."
Status:
ReviewerMember since: 2016
Organization: Universidad de Cordoba
The results are good enough, but the efficiency rate could be improved.
Application Area:Analyses of DNA methylation
"The kit is efficient enough and it worked well for me. Sometimes the efficiency is above the threshold (I mean, the controls were not totally converted), but this happened few times (15-20%). I think this could be improved but it is so easy to use that I still considering the best option. "
Status:
ReviewerMember since: 2016
Organization: Genome Quebec
very good product for methylation analysis
Application Area:service provider
"clean and constant product, very good support for developing testing "
The EZ DNA Methylation-Gold™ Kit is a refinement of our popular EZ DNA Methylation™ kits.
These products consolidate DNA denaturation and bisulfite conversion processes into one step, leading to a much faster bisulfite conversion. This is accomplished using temperature denaturation to complement chemical denaturation in the previous protocol. Also, this kit have been streamlined for high yield recovery of DNA following bisulfite treatment. Recovered bisulfite-converted DNA is ideal for PCR amplification for downstream analyses including endonuclease digestion, sequencing, microarrays, etc.