Dual-Luciferase ® Reporter Assay System by Promega Corp.

Manufacturer Promega Corp.
4.4
/
5.0
  |  10 reviews
The Dual-Luciferase® Reporter (DLR™) Assay System(a–f) provides an efficient means of performing two reporter assays. In the DLR™ Assay, the activities of firefly (Photinus pyralis) and Renilla (Renilla reniformis or sea pansy) luciferases are measured sequentially from a single sample. The firefly luciferase reporter is measured first by adding Luciferase Assay Reagent II (LAR II) to generate a luminescent signal lasting at least one minute. After quantifying the firefly luminescence, this reac... Read more


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Dual-Luciferase ® Reporter Assay System
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Average Rating: 4.4
10 Scientists have reviewed this product

5 out of 5
Ease of use
5 out of 5
After sales service
4 out of 5
Value for money


  • Status:

    Reviewer
  • Member since: 2019

  • Organization: University of Maryland



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    3 out of 5
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Good and reliable results.
Rating: 4.0

  • Application Area: Viral neutralization assay

"It's a well established system and there are many references to support the assay. Therefore it's pretty reliable and the results were pretty reproducible. The price was fair but still high cost considering our high sample amount."

Review date: 28 Oct 2019 | Dual-Luciferase ® Reporter Assay System
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Earli



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
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Excellent product, but a bit pricey.
Rating: 4.7

  • Application Area:Characterize promoter activity

"Kit works great, we scale down the volumes a bit since the reagents are so expensive. We also have the GloMax plate reader which is excellent to use."

Review date: 25 Oct 2019 | Dual-Luciferase ® Reporter Assay System
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: UTHSCSA



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Your products are very practical, good quality, and high efficiency.
Rating: 5.0

  • Application Area:Caspase-glo3/7 assay

"A high sensitivity caspase assay that requires fewer cells and less enzyme."

Review date: 25 Oct 2019 | Dual-Luciferase ® Reporter Assay System
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: University of Colorado



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    3 out of 5
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Easy to use and effcient.
Rating: 4.3

  • Application Area:Molecular biology/neuroscience

"This product is awesome. It is easy to use and reliable. However, I do think it is highly priced."

Review date: 25 Oct 2019 | Dual-Luciferase ® Reporter Assay System
  • Status:

    Reviewer

  • Member since: 2011

  • Organization: UT MD ANDERSON CANCER CTR.



  • Ease of use
    3 out of 5
    After sales service
    4 out of 5
    Value for money
    2 out of 5
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Rating: 3.0
"This kit is expensive and not very easy to use, but is necessary for some of our research as there are not a lot of kits like this in the market."

Review date: 12 Jul 2011 | Dual-Luciferase ® Reporter Assay System
  • Status:

    Reviewer

  • Member since: 2011

  • Organization: Southern Illinois University



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
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Rating: 4.7
"Easy to use."

Review date: 11 Jul 2011 | Dual-Luciferase ® Reporter Assay System
  • Status:

    Reviewer

  • Member since: 2011

  • Organization: INST. OF ONCOLOGY ANGEL H. ROFFO



  • Ease of use
    4 out of 5
    After sales service
    3 out of 5
    Value for money
    4 out of 5
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Rating: 3.7
"Really easy to use product, reproducible results."

Review date: 06 Jul 2011 | Dual-Luciferase ® Reporter Assay System
  • Status:

    Reviewer

  • Member since: 2011

  • Organization: H. LEE MOFFITT CANCER CTR.



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Rating: 5.0
"Very easy to use and has a long shelf life."

Review date: 05 Jul 2011 | Dual-Luciferase ® Reporter Assay System
  • Status:

    Reviewer

  • Member since: 2007

  • Organization: Neuromed



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Rating: 5.0

Review date: 09 Jul 2007 | Dual-Luciferase ® Reporter Assay System
  • Status:

    Reviewer

  • Member since: 2007

  • Organization: Queen's University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Rating: 5.0

Review date: 11 Jun 2007 | Dual-Luciferase ® Reporter Assay System
The Dual-Luciferase® Reporter (DLR™) Assay System(a–f) provides an efficient means of performing two reporter assays. In the DLR™ Assay, the activities of firefly (Photinus pyralis) and Renilla (Renilla reniformis or sea pansy) luciferases are measured sequentially from a single sample. The firefly luciferase reporter is measured first by adding Luciferase Assay Reagent II (LAR II) to generate a luminescent signal lasting at least one minute. After quantifying the firefly luminescence, this reaction is quenched, and the Renilla luciferase reaction is initiated simultaneously by adding Stop & Glo® Reagent to the same sample. Both assays can be completed in about 4 seconds using a luminometer with reagent auto-injectors. In the DLR™ Assay System, both reporters yield linear assays with attomole (<10−18) sensitivities and no endogenous activity in the experimental host cells. Furthermore, the integrated format of the DLR™ Assay provides rapid quantitation of both reporters either in transfected cells or in cell-free transcription/translation reactions.

The pGL4 and phRL series of synthetic Renilla Luciferase Reporter Vectors are designed for use with the DLR™ Assay Systems. A Renilla luciferase vector with constitutive expression may be used in combination with any experimental firefly luciferase vector to co-transfect mammalian cells.