DNA Ligation Kit for Long Fragments
Takara's DNA Ligation Kit for Long Fragments is a powerful tool for the cloning of long DNA fragments spanning 2 kb to over 10 kb in length. The kit contains an optimized ligase/buffer system that enables ligation of long fragments without the need for extensive techniques or special expertise. It is especially well suited for BAC library construction. Features: Superior ligation efficiency, especially for long DNA frag…
The supplier does not provide quotations for this product through SelectScience. You can search for similar products in our Product Directory.
Takara's DNA Ligation Kit for Long Fragments is a powerful tool for the cloning of long DNA fragments spanning 2 kb to over 10 kb in length.
The kit contains an optimized ligase/buffer system that enables ligation of long fragments without the need for extensive techniques or special expertise. It is especially well suited for BAC library construction.
Features:
- Superior ligation efficiency, especially for long DNA fragments
- Simple protocol
- Long control vector and insert provided
Applications:
- Vector construction using long DNA fragments
- BAC library construction
- Cloning of long cDNA fragments
Using TaKaRa DNA Ligation Kit LONG for Efficient BAC Library Construction Using Mammalian Blood Samples
When ligating DNA fragments over 10 kb in length, the ligation efficiency will be very poor if a conventional ligation kit is used. DNA Ligation Kit LONG includes an optimized ligase/buffer system that enables you to ligate long DNA fragments without need for specialized expertise or extensive optimization. This application note describes the use of TaKaRa DNA Ligation Kit LONG to construct a BAC library with genomic DNA inserts from mammalian blood samples.
