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Using TaKaRa DNA Ligation Kit LONG for Efficient BAC Library Construction Using Mammalian Blood Samples

Takara Bio
22 Jan 2016

When ligating DNA fragments over 10 kb in length, the ligation efficiency will be very poor if a conventional ligation kit is used. DNA Ligation Kit LONG includes an optimized ligase/buffer system that enables you to ligate long DNA fragments without need for specialized expertise or extensive optimization. This application note describes the use of TaKaRa DNA Ligation Kit LONG to construct a BAC library with genomic DNA inserts from mammalian blood samples.

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DNA Ligation Kit for Long Fragments

Takara Bio

Takara's DNA Ligation Kit for Long Fragments is a powerful tool for the cloning of long DNA fragments spanning 2 kb to over 10 kb in length. The kit contains an optimized ligase/buffer system that enables ligation of long fragments without the need for extensive techniques or special expertise. It is especially well suited for BAC library construction. Features: Superior ligation efficiency, especially for long DNA fragments Simple protocol Long control vector and insert provided Applications: Vector construction using long DNA fragments BAC library construction Cloning of long cDNA fragments

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Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.Gene Expression and Molecular CloningMolecular cloning is a set of techniques that utilizes vectors to transfer recombinant DNA into host cells and is an essential tool for investigating the expression of genes and proteins in bacterial or mammalian cells. A variety of vectors optimized for gene cloning and expression in a range of host organisms are available, alongside competent cells for genetic replication. Here, you can explore a range of molecular tools, high-quality genomic and cDNA libraries, premade clones, transformation and transfection reagents and mutagenesis or gene expression detection assays and expression arrays. Find the best gene expression and molecular cloning products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Library PreparationLibrary preparation is a critical step in sequencing workflows, where DNA or RNA samples are converted into libraries for high-throughput, next generation sequencing. This step ensures accurate results and minimizes biases. Explore library preparation kits in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.

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Using TaKaRa DNA Ligation Kit LONG for Efficient BAC Library Construction Using Mammalian Blood Samples