Direct-zol™ RNA Miniprep Plus by Zymo Research

"Using the Direct-zol RNA mini-prep plus kit, I was able to yield good quality and high concentrations of RNA from C. elegans lysates, allowing me to send for RNA-seq."

"I have been using Zymo's Direct-zol kit for 6 years now and absolutely love it. The price to value is unbeatable. As a graduate student, my PI made us compare results to the standard chloroform spin-down method for both purity and cost. There was nearly no comparison. If you care about purity, saving time, and simplicity, there isn't another option I would recommend."

"The reagent is cost effective and easy to use. Purified RNAs are of great quality and reasonable quantity."

"My lab has been using this product for many years. It is easy to use, robust, and with a high yield. More importantly, it also captures small RNA, so we can quantify both protein-coding RNA and miRNAs in the same sample. After beginning using this product about 10 ago, we had never used other similar kits, nor will switch to other kits in the future."

"After some optimization, the kit worked well with consistent results. The Support team was amazing."

"Great yield."

"The RNA yield was good!"

"Very clean RNA with consistent results. Protocol is easy to follow with good support from the company. The DNase in column treatment works well and saves time downstream. "

"Kit is easier and quicker to use than alternatives with more consistent results. It is also considerably more affordable than other comparable kits."

"The RNA miniprep kit is very easy to use and I got excellent yield and quality of RNAs. Very happy with the kit."

"This is a very easy to use kit for RNA isolation from tissue and cells. A straight forward protocol makes it easy to process multiple samples in a small amount of time. The RNA is of high quality and the data are reproducible and reliable."

"Very easy and straight forward protocol. Yield good quantify and high quality of RNA. Good value for the price compare to some other RNA extraction kits."

"Achieved high quality yield of DNA. Easy to follow protocol and user's friendly. The price is also cheap"

"My samples are very challenging to lyse pathogenic yeasts. I was used to work with phenol but often had contamination due to our in house protocol. This kit optimized my workflow from 2 days to 20 min experiment"

"This kit provides high yields for an affordable value. Moreover, what I like about all Zymo kits is that you can order just one of the components if it runs low, while in most others you have to buy a new kit!"

"We use Zymo RNA extraction kit to extract RNA from monosome fractions. The quality of the RNA using the kit is excellent, which is so important for downstream library preparation. The kit is also easy to use."

"That product is effective in my research, for sequencing DNA or proteins, analyses of DNA, very easy to use, good quality and stable."

"It's an easy of use product, good balance between value and money"

"Easy to use, with great results."

"The kit is easy to be used, the final product (RNA) quality was excelent."

"DirectZol is super easy to use and I routinely get excellent quality total RNA (e.g., RINs 9-10)."

"Compared to other RNA isolation kits, the Direct-zol RNA Miniprep plus maximizes the amount of RNA isolated from a small sample size. The ability to go directly from TRI Reagent/trizol along with the on-column DNA digestion step reduces sample handling to minimize RNA loss. The RNA is of high quality allowing for several different downstream uses."

"Compared to other RNA isolation kits, the Direct-zol RNA miniprep plus maximizes the amount of RNA isolated from a small sample size. The ability to go directly from TRI Reagent/trizol along with the on-column DNA digestion step reduces sample handling to minimize RNA loss. The RNA is of high quality allowing for several different downstream uses."

"I love Zymo products and I cannot wait to open my own lab someday and use exclusively Zymo reagents. I have never had issues with their products and this RNA miniprep kit works wonders for Trizol reactions. It is very dynamic and have gotten me great yields for many papers so far."

"This kit produces high quality RNA, which can be concentrated using a small volume of elution buffer; no need concentrating afterwards."

"I used the Direct Zol RNA miniprep kit to isolate total RNA from my bacterial cultures. I was able to receive a high yield with no problem and the kit only takes 20 -30 minutes in total to finish up the whole process. We have purchased this kit multiple times and I highly recommend it as well."

"Great results! Easy to use, very reproducible"

"Easy to use, it gives high quality DNA yield, it can be used for PCR applications, it gives reliable results. Good efficiency in time and money saving."

"It has better quality and yields RNA. The protocol is easy and simple and the result is consistent among samples."

"They came on time. Got good quality and yield of RNA. Would highly recommend."

"I used Direct-zol RNA Miniprep Plus to extract RNA from plant samples. It ended up in high quality and quantity."

"The kit worked well and was easy to use. I analyze brain tissue (hippocampus and amygdala) and needed to wash multiple times to eliminate fat. Otherwise was able to use standard protocol that came with kit."

"Zymo Research products are by far the easiest kits to use and are extremely effective without and surprises."

"I really appreciate your products which saved a lot of time for me. As a graduate student and a mother of two kids, time is everything. I love your products. By the way, separated purchase available is very consumer friendly. Like the choice a lot."

"The Zymo RNA MiniPlus kit is very user-friendly. It achieves RNA isolation through a column-based approach. The benefit is a time-saving prep that yields high-quality RNA as analysis by NanoDrop spectra. The one limitation is the column lowers the total RNA yield. Flushing of the column helps to recapture total RNA. This is a great alternative to traditional Trizol and phenol: chloroform isolation protocols which require special waste procedures for both phenol-containing liquid and plastic waste."

"This kit is quite easy to use. The RNA quality is very good. The workflow is very easy and quick. The only thing about it that is a little frustrating is that the tubes a slippery and not so easy to handle. They dont have good grip. You have to be very careful or they'll slip out of your fingers."

"Direct-zol RNA Miniprep Plus Kits are great! I'm very satisfied with the efficiency/quickness of RNA extraction from human brain samples, especially when we need high quality RNA for quantification and sequencing. This product is extremely easy to use and provided a good purity of the extracted RNA. And finally, the technical support were very helpful and very responsive."

"The instructions were easy to follow and I got a good and pure yield for the PCR experiment I needed to conduct using the RNA."

"The TRI-reagent should always be handled inside of a fume-hood. If you are planning to work with more than 20 samples, a second kit is necessary to have enough of the flow through columns."

"This kit is very easy to use and quick to use. I was able to achieve high yield with clean results time after time. I was able to use it for blood, cheek swabs, and saliva. Anytime I called support, they were very helpful and very responsive. I couldn't be happier."

"I used this kit to extract RNA from insects and need to detect a RNA virus from it. The kit is very easy to use, save time, and all can be performed under room temperature. I can extract RNA from a singe insect as well as a pool of insects. The chemical reagents within the kit are super enough for extraction uses. This kit is cheaper than Qiagen kit, so it is more affordable. And the extraction result is also very good! BTW, the technical support is very helpful!"

"Good value for money. Zymo doesn't rip you off, unlike other companies."

"This product is extremely easy to use. I love that all of the needed reagents come prepared and aliquoted for continued ease of use. The protocols are easy to follow and provide reproducible results."

"The product is quite user friendly. The results were reproducible and high quality. Everyone should know about the great Zymo support team who helps when needed as far as technicalities are concerned. It is a 5/5 for me in all aspects."

"The DirectZol kit is great (most of the time). The RNA yields from cell culture are reproducible and the purification process is a "piece of cake." Anyone with novel experience can use this kit and get great results FAST."

"If I place order the product today, then I can receive it the next day. After using it, the product is high quality and shows my result. Compare with other vendors, the price is a reasonable. Thank you."

"The samples were clean and the concentrations were good."

"I have used this kit for the isolation of RNA from a small number of sorted cells. It worked great, we sent it for RNA-seq and got incredibly good results."

"A great kit to increase the quantity and purity of the extracted RNA. I am very happy to use it for my lab samples which needs high quality RNA for quantification and sequencing. Very amazed with the productivity of this kit."

"Our lab tried this product after years of Trizol/Chloroform extractions. We needed a kit that would produce *clean* mRNA with little Trizol carryover for downstream RNAseq applications. This kit did its job well and produced clean, pure mRNA and is less expensive than RNeasy and RNaqueous counterparts. The Zymo website also provides a brief video of the protocol which we found to be a helpful introduction. Our procedure, briefly: After grinding the tissue in liquid nitrogen and adding Trizol, we passed the mixture through a Qiashredder. After pelleting the debris, the Trizol/lysate supernatant was put through the Direct-Zol kit."

"It is easy to use. Great quality end product."

"I had to extract 50 samples for total RNA, these samples had to be sequenced for splice variants. Considering that the number of samples were quite a few, I did opt for the traditional method nor the Qiagen (as the DNAse treatment is a column and not a physcial enzyme). I used the RNAzolkit, and within 15 mins is at least 95% of the samples with RIN of >7. This kit is rather easy to use with the DNAse I digestions step in the column itself, so that was good. The NGS run was great so this one is good especially because you can also store your RNA in TRIZOl and extract the RNA whenever you want without the fear of degradation."

"It is really very easy to use, we just had the problem with the sample homogenization, we were working with pancreas tissue and the RNA was easily degraded, but this kit was the option with one we got the best result using a homogenization with trizol in low temperature."

"Product was effective and easy to use. Price is also okay"

"Direct Zol was the best tool for RNA isolation it helps to achieve high quality and purity from my samples also reduces the time consuming compared with the traditional method"

"An excellent method to quickly isolate high-quality RNA directly from samples in Trizol, TRI Reagent or similar from different sample sources (cells, tissues, serum, plasma, etc.)."

"I used Zymo's Direct-zol RNA miniprep kit for the isolation of RNAs from specific caterpillar tissues for Next Gen Sequencing. Because I was isolating RNAs from multiple different tissues of each individual, I had to process 100s of samples! I was able to rapidly isolate high-quality RNA from the tissues and obtain high yields. This kit really saved the day when it came to processing 100s of tissue without losing any quality of isolated RNA in the sample!"

"The Miniprep Plus was extremely good at extracting RNA in sufficient concentrations. The instructions were also very clear, but I think it would have been beneficial to have more volume of each solution. Other than that, it was great!"

"The RNA yield obtained using the kit was great and the purity was ok to perform any throughput analysis."

"I achieve high quality RNA that is used as an input for RNA sequencing."

"The sample kit is a good start for my first trial of RNA prep and it is very useful for me to compare different kit based on my sample size."

"The kit is very easy to use, even my undergraduate students can finish the experiments very well and fast, the RNA quality and yield is pretty high. The price of this kit is reasonable."

"The product is very easy to use, in a relatively less amount of time with better RNA quality."

"The protocol is straightforward and easy to use. Compared to red/blue it's also relatively cost effective. The yeild concentrations are spectacular!"

"I like the capacity of the kit. It provides good results and very easy to use. Highly efficient and cost effective. Very happy to have and would recommend for the best RNA procedure."

"This item is very useful for the quick extraction of tissue RNA samples with high purity."

"Anyone who has tried RNA extraction from tissue/cell lines can attest to the frustrations that may be present throughout the process. From low yield to degradation, RNA extraction can be very challenging using any method. This kit was surprisingly easy to use, and gave great results. Will definitely be purchasing the full kit for future use."

"This prep kit is easy to use and has all the materials. We were able to isolate the RNA in good concentrations, which we then used for our projects."

"Unfortunately, this kit did not allow me to purify sufficient RNA from a tough-to-lyse bacterium that makes some unknown biofilmy stuff. However, it did result in high yield RNA from the alga chlamydomonas reinhardtii, albeit the quality assessed via bioanalyzer trace was just OK (not perfect)."

"Great kit. Easy to use and reliable results. Perfect for NGS applications."

"Very easy to extract RNA, simple fast and good concentrations."

"The Direct-zol kit is very useful for extracting DNA from bacteria, and it worked just so well for our thermophilic cultures. The results and yields were reproducible too, and the transcriptomic data that was processed using the RNA extracted from RNA zol was of high quality."

"Using this kit to obtain RNA is easy and very effective. You can get higher yield and purer RNA. The RNA obtained is great for downstream application, such as RT-PCR, RNA sequencing, and gene cloning. Customer service is great too, very informative and helpful."

"Rapid and reliable isolation of RNA for qRT-PCR analysis in plants."

"We had existing samples that were frozen in tri-reagent and these kits worked well for extracting high quality RNA."

"This product is very easy to use. The protocol is simple to follow. I got great RNA quality and quantity. The qRT-PCR Results from the RNA were very clean"

"Excellent to purify RNA in an easy and fast way with high quality and quantity RNA."

"The kit has been working very well with consistent results with good RNA quality and yield. The spun columns are handy compared to the Trizol method. The procedure is straightforward to follow as well."

"Very interesting, feasible, and highly efficient method for RNA extraction from adipose tissue."

"RNA quality is very good and the kit is very easy to use, which makes the whole procedure more reproducible even in unexperienced hands. You can quickly get high quality RNA without worries of losing your RNA pellet."

"Direct Zol is a simple and robust kit to get your RNA out of cells. You may better off with your turboDNAse treatment, althugh kit has its own DNAse in it and recomended to use following the protocol."

"This kit is easy to use and we can get enough amount of RNA for RT-qPCR from mouse skeletal muscle tissues and cultured cells. The protocol is simple and we do not need any special equipment for this kit. The price is not as expensive as other similar kits."

"This kit was very useful to extract RNA from cancer cells to use for qPCR experiments. The instructions were easy and efficient!"

"I have used so far 10 Direct-zol RNA Miniprep Kits to purify RNA from adherent and suspension cell samples lysed in Trizol and always performed the optional on-column DNAse digestion. The yields were extremely good, most samples show RNA yield more than expected over OD 260/280 2.0. I stopped using big brand kits, which take a much longer time to purify with lower yields and this Direct-zol RNA Miniprep Kit seemed like such a convenient alternative."

"It is easy to use, reliable, and accurate. Good for beginners as well."

"Easy to use, reproducible results, and no special aftercare needed."

"I find the Direct Zol RNA isolation kit the fastest, easiest, and highest yielding method for isolation of RNA from Trizol extracted Drosophila tissue lysates. So much easier than centrifugation followed by phase separation. I've also started using the kit to clean up capped mRNA T7 ARCA transcription reactions, and the yields are wonderful! Usually get 600 to 700 ng/microliter of clean useable mRNA for insect transgene injections. I highly recommend this product."

"Is really easy to use and reduces steps on the traditional method to obtain pure RNA."

"We’ve been working to generate pipeline with bulk RNA seq. Our lab tried several kits from different companies, then decided to purchase the entire kit from Zymo after trying a sample kit. This kit gave us highest amount of RNAs with hight quality for RNA-seq from both whole tissue and cells collected from FACS. The columns works perfect with Trizol agent-which is the most common reagent and in-column digestion to remove DNAs was another benefit! Easy to follow the directions and reasonable price for 50 preps, compared to other competitors. Highly recommend."

"Direct-zol™ RNA Miniprep Plus is excellent value for money over similar products."

"It was easy to manipulate, product isolated was good on both yield and quality."

"The RNA yield was better than other products, but the column-based method does not have a high throughput. It would be helpful if the product can be improved for automated pipelines."

"Gives you great quality RNA in high concentrations every time! It’s very simple and easy to use and instructions are crystal clear. "

"It is easy to use, you can use trial in the beginning. The protocol is short and effective. Technical support is good."