Direct-zol™ RNA Miniprep Plus by Zymo Research

Manufacturer Zymo Research  |   Model: R2070
4.7
/
5.0
  |  71 reviews
The Direct-zol™ RNA Kits provide a streamlined method for the purification of up to 100 ug (per prep) of high-quality RNA directly from samples in TRI Reagent® or similar. Spin column procedure bypasses phase separation and precipitation procedures, for non-biased recovery of miRNA.


Direct-zol™ RNA Miniprep Plus by Zymo Research product image
Direct-zol RNA Miniprep Plus Kit
Buy Now

Place your order directly with the manufacturer.




Average Rating: 4.7
71 Scientists have reviewed this product

5 out of 5
Ease of use
4 out of 5
After sales service
5 out of 5
Value for money


  • Status:

    Reviewer
  • Member since: 2019

  • Organization: University of Arizona



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Worked well and saves us a lot of time!
Rating: 5.0

  • Application Area: RNA preparation for RNA seq

"This kit is super easy to use and is very convenient. Before this, we were using the trizol-chloroform method, which was messy and took a lot of time for the number of samples we usually prepare."

Review date: 21 Jun 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: NIH



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Great result
Rating: 5.0

  • Application Area:RNA isolation and qPCR

"Always reliable results and fast and easy procedures"

Review date: 20 Jun 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: OKPI



  • Ease of use
    5 out of 5
    After sales service
    1 out of 5
    Value for money
    5 out of 5
Good RNA quantity, but beware of DNA contamination.
Rating: 3.7

  • Application Area:RNA isolation

"Easy to use, large quantity of RNA, however DNA elimination is not complete. Bashing beads are necessary to complete lysis of cell walls."

Review date: 13 Jun 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Franklin Pierce U



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Reliable and more cost effective than many spin column kits.
Rating: 4.7

  • Application Area:Isolate RNA or DNA from C.elegans

"We have been using the Direct-zol™ RNA Miniprep Plus kit for a couple of years now. The cuticle of nematodes is a little difficult to deal with sometimes, but we have had consistent quality of RNA from the kit. DNA is even easier."

Review date: 11 Jun 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Texas Tech University Health Sciences Center



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Easy to use and great results!!
Rating: 5.0

  • Application Area:Clinical molecular diagnostics

"It is easy to use and a very fast procedure. In addition to that, it results in great yield and quality."

Review date: 11 Jun 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Virginia Commonwealth Uni



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
It's very easy to use, time-saving and has fewer steps. Will definitely buy again.
Rating: 5.0

  • Application Area:RNA isolation and purification

"It's very easy to use, time-saving and has fewer steps. Will definitely buy again."

Review date: 07 Jun 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: RTLGENOMICS



  • Ease of use
    4 out of 5
    After sales service
    3 out of 5
    Value for money
    3 out of 5
Works well for a specific sample types, but no easily adapted to others.
Rating: 3.3

  • Application Area:Isolate RNA from samples

"It works well for tissue only but it’s not easily adapted for other sample types. I work with a variety of sample types and would like a kit that’s easily adaptable to the sample type I’m working with."

Review date: 07 Jun 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Texas A&M Health Science Center



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Easy to use kit for reproducible quality results.
Rating: 4.3

  • Application Area:RNA extraction from small sample sizes of neurons for downstream applications, including RT-qPCR

"This product is single tube and uses a bench-top centrifuge for a very quick and easy extraction process. We have been able to achieve a decent yield and reproducible quality from this kit, on a variety of starting sample sizes and from both neurons and astrocytes."

Review date: 06 Jun 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Universidad Poliécnica del Valle de Toluca



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Excellent product.
Rating: 5.0

  • Application Area:Isolation of viral RNA from human blood

"The product is easy to use and we obtained a very good amount of RNA from human blood. It's also not expensive."

Review date: 06 Jun 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Charite CBF Institute of Medical Immunology



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Highly recommended for CRISPR editing.
Rating: 5.0

  • Application Area:In-vitro transcription purification

"We were able to obtain a free kit to test and were able to retrieve high yields of RNA from in-vitro transcription."

Review date: 06 Jun 2019 | Direct-zol™ RNA Miniprep Plus


  • Status:

    Reviewer

  • Member since: 2019

  • Organization: SupadElixir Co., LTD



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Solid and gives great results.
Rating: 5.0

  • Application Area:Test the knockdown efficiencies of shRNA or siRNA

"It was easy to use, worked well, came out with good results, and is even cheaper than some competitors."

Review date: 06 Jun 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: INRA



  • Ease of use
    4 out of 5
    After sales service
    3 out of 5
    Value for money
    3 out of 5
More reliable results than comparable kits.
Rating: 3.3

  • Application Area:Analyze of RNA expression in animal tissue

"Easy of use. Manufacturer recommendations are clear enough. Safety data sheet OK."

Review date: 06 Jun 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Leibniz Zentrum für Marine Tropenforschung



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Easy and fast RNA extraction.
Rating: 5.0

  • Application Area:RNA extraction for gene expression study

"The RNA kit is easy to handle and fast to use. Otherwise we only use Trizol, but this time we switched to a kit from Zymo Research. The RNA yield was very good, the quality too. Inhibitors from the sample matrix were not observed. The cDNA synthesis proceeded without complications. We recommend this kit."

Review date: 06 Jun 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: West Virginia State University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Easy to use, fast, and high quality RNA.
Rating: 5.0

  • Application Area:RNA-seq from fruit tissues

"It was very helpful because I was able to extract RNA with a very good quality in a very short time."

Review date: 06 Jun 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: NWP



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
This kit worked great for RNA extraction from plant material.
Rating: 4.7

  • Application Area:Plant virus identification and diagnostics

"This kit was purchased for RNA extraction from plant material for work on a low titre virus. RNA was used for PCR and we were able to get consistent and repeatable results."

Review date: 06 Jun 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: University of Arizona



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Great experience, made our experiments successful.
Rating: 5.0

  • Application Area:Molecular signaling pathway analyzing in cortex and neurons

"We got a very good quality of RNA from limited amount of specific area in cortex. The price of the kit is very good for research experiments. Moreover, the protocol is much easier to carry out and saves a lot of time. It does not require the centrifuge to be cooled to 4 degrees any more, which is very convenient for most labs. Our students like the kit very much compared to the previous classical one. We would like to order more in future."

Review date: 05 Jun 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Universidad de Valparaiso



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Trizol improved with Direct-Zol.
Rating: 5.0

  • Application Area:Analyzing microRNA expression in tissue and cell lines

"We have been using Trizol following the manufacturer indications to extract RNA from tissue and cell lines. However, we wanted to improve the extraction in terms of purity while reducing the hands on time. We found out about Direct-Zol and tested it. Our RNA improved in quality and we reduced the time for extraction. In addition there's no need for chloroform addition, which is outstanding."

Review date: 05 Jun 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Instituto de Neurobiología, UNAM



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Very useful kit.
Rating: 4.7

  • Application Area:Analyze mRNA in tissue samples

"Direct-zol has been very effective to isolate total RNA highly purified to use as a template to the synthesis of cDNA. For purposes to amplified samples by qPCR, high quality samples of RNA are obtained easily and quickly. The value for money is very good, the time required, the quantity of sample and the easy way to perform the procedure justify the cost."

Review date: 05 Jun 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2010

  • Organization: The University of Arizona



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Great results! I directly used those RNA samples for RNA-Seq.
Rating: 5.0

  • Application Area:Analyze gene expression from maize mesophyll protoplasts

"This is the first time I've used this new RNA extraction kit, so I had a lot of questions to ask. The customer service did a great job answering my questions. The steps of this protocol were quite easy to follow and I got good quality RNA."

Review date: 05 Jun 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: East Carolina University Brody School of Medicine



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
Very good yield.
Rating: 4.7

  • Application Area:Extract RNA from cultured cells and analyse gene expression

"Consistent data and easy to use. Had no problem in following the protocol."

Review date: 05 Jun 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Dana-Farber Cancer Institute



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Great results, and great value for your money!
Rating: 4.7

  • Application Area:RNA extraction

"I am extremely satisfied using the Direct-zol RNA MiniPrep Plus kit! I no longer have to worry about the days of using the dreaded chloroform/Trizol extraction method which would sometimes take hours to finish. The Direct-zol protocol is easy, efficient, and can be finished in less than 30 minutes! Now I can extract RNA and run a qPCR for the same samples all within the same day! I love using the Direct-zol kit and can't imagine doing RNA extraction without it!"

Review date: 05 Jun 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Modular Bioscience



  • Ease of use
    5 out of 5
    After sales service
    1 out of 5
    Value for money
    3 out of 5
It is ok quality but not that great.
Rating: 3.0

  • Application Area:Transcriptome

"It is fast but the quality is not that great. I found some smears after use."

Review date: 05 Jun 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: CHOC Children's Hospital



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Good quality with reasonable price compared to competitors' products.
Rating: 5.0

  • Application Area:RNA extraction.

"Good quality with a reasonable price compared to competitors' products. The results are comparable, but the price is significantly lower."

Review date: 05 Jun 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Nilogen Oncosystems



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
When my samples had RNA it worked great.
Rating: 5.0

  • Application Area:RNA extraction

"Super easy to use! RNA was clean upon extraction for our downstream assays."

Review date: 05 Jun 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: MilliporeSigma



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Great results.
Rating: 4.3

  • Application Area:RNA extraction

"The product was easy to use and gave me good quality RNA. It was good value and delivered quickly."

Review date: 05 Jun 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: University of Illinois



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Great product for RNA related works.
Rating: 5.0

  • Application Area:Measure miRNA and mRNA expression in cells and animal tissues

"We think the protocol that comes with the kit is very easy to read and follow, even first year undergraduate researchers can easily get familiar with the procedures, which increases the lab's efficiency greatly in terms of cutting down the training time. Also it always yields good RNA concentration, for both cells and animal tissues. We deal with mouse colon tissue a lot and due to the small portion we have, we really appreciate this product and its high yield rate with good quality control. Ever since we started to use this product, we have conducted several animal studies and published multiple manuscripts with it, and we will continue using it in future."

Review date: 05 Jun 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Clemson University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Go to for all our RNA extractions.
Rating: 5.0

  • Application Area:Zebrafish, chicken, development, RT-qPCR, PCR, NGS

"As a developmental biologist in a mostly undergraduate university, I need a product that delivers high-quality RNA in a format that is highly reproducible and cost-effective. My undergrads find the protocol easy to follow as it has far fewer steps than competitors' products. Students' results are more consistent between experiments because of the simpler format. The end product is of high quality and we have used the RNA for qPCR and NGS experiments successfully. My ratings are all five star. The kit is much cheaper than those of other major competitors and after sales service is excellent."

Review date: 05 Jun 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Texas A&M University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Good concentration of quality RNA was extracted with ease.
Rating: 5.0

  • Application Area:RNA extraction

"It's a very simple and straightforward kit to use. We used it to extract RNA from sorghum leaf and internode tissue which is very hardy but this kit made it easy to extract good quality RNA isolation for qRT PCR and RNA Seq applications."

Review date: 05 Jun 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: University of North Dakota



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
Amazing product. This product improved the quality of my RNA greatly.
Rating: 4.7

  • Application Area:RNA isolation from tissue and cells

"This is a great product. Design of column prevents alcohol or trizol contamination in final RNA. Quality of RNA is great and never seen any DNA in RNA prep. This product is good for small RNA also. It gives reproducible result."

Review date: 05 Jun 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Pennington Biomedical Research Center



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Great quality for a great price.
Rating: 5.0

  • Application Area:RNA isolation

"This kit has not only saved us money compared to other similar kits (which are usually hundreds of dollars more) but is also both easy to use and produces great results."

Review date: 05 Jun 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Albert Einstein College of Medicine



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Performance of kit is great. I always have it on my shelf.
Rating: 5.0

  • Application Area:Isolate RNA including miRNAs for further total transcriptomics analyses

"RIN =10 for all RNA preparations, good yield of miRNAs, results are highly reproducible, kit is easy to use. It is compatible with any lysis buffer. The cost is much better or comparable with similar products from other vendors. However it's performance is much better. Technical support is readily available but I really never needed it as the kit works without failure exactly as is suggested by the manual."

Review date: 05 Jun 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Harvard Medical school



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Super effective.
Rating: 5.0

  • Application Area:Extract RNA

"Reproducibly high quality. Very easy to use. Easy to scale up too."

Review date: 05 Jun 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: UConn



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
I will buy it again if there is a promotion.
Rating: 4.3

  • Application Area:Extract plasmd from competent cells

"Generally it's good. The miniprep steps saved us much time. The extracted product has high purity but low yield."

Review date: 05 Jun 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Naxxis



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Good results.
Rating: 4.0

  • Application Area:DNA/RNA analysis

"Hight quality DNA/RNA purification. Provides good results for epigenetics."

Review date: 05 Jun 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: NATIONAL INSTITUTE OF BIOLOGY



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
Easy to use, effective and retains sRNAs.
Rating: 4.7

  • Application Area:Nucleic acid isolation

"The kit is easy to use and the isolated RNA is of high quantity and quality. Important, by using these columns you don't lose small RNAs."

Review date: 05 Jun 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Penn State Harrisburg



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Great results obtained quickly!
Rating: 5.0

  • Application Area:RNA isolation

"Easy to use, quicker protocol than most other vendors, great, reproducible results!"

Review date: 05 Jun 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: University of Rochester School of Medicine and Dentistry



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
Great results, works well.
Rating: 4.7

  • Application Area:Extract RNA from tissue culture supernatant

"This kit was very easy to use. I received high quality and reproducible results. Worked for exactly what I needed."

Review date: 05 Jun 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Rutgers University



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Happy with the results.
Rating: 4.0

  • Application Area:RNA extraction in NK cell line

"I had problems getting good quality RNA in NK cells and found an easy and clean way to use trizol extraction."

Review date: 05 Jun 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: The George Washington University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
I really like this kit & recommend for anyone to use over acidic phenol or other kits.
Rating: 5.0

  • Application Area:RNA collection from mammalian cells to reverse transcribe DNA.

"The kit was very easy and a welcome change from acidic phenol. I was surprised how good the yield and quality of the RNA was. The RNA was great and I obtained the reverse transcribed DNA the first time and it was easy to subclone."

Review date: 05 Jun 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: University of Louisville



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Great results.
Rating: 5.0

  • Application Area:Analyzing reproductive tissue samples

"Achieve excellent results, easy to use, cost effective."

Review date: 05 Jun 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Van Andel Institute



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Great result, super easy!
Rating: 5.0

  • Application Area:Evaluation of mRNA levels for certain signaling pathways

"This kit helps me transfer from traditional trizol purification steps with simple column loading and washing. It is cool! I do have a suggestion: is there any possibility to omit the extra DNase digestion step and provide a more efficient DNA elimination column before RNA purification column?"

Review date: 05 Jun 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: University of South Carolina



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Great results. I get high yield transcriptome ready RNA in each sample.
Rating: 5.0

  • Application Area:Analyzing gerbil stomach

"Very easy to use. Zymo makes great kits. Very affordable pricing."

Review date: 05 Jun 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: ISU



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
This kit is easy to use and yields good quality and quantity of total RNA.
Rating: 5.0

  • Application Area:Next Generation Sequencing

"Works great even for the most degraded samples. Easy to use and very fast."

Review date: 05 Jun 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Pennsylvania State University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Great Results: the perfect complement to Trizol.
Rating: 4.7

  • Application Area:Purification of DNA and RNA from insect tissue

"This is a great easy to use kit to purify high quality DNA and RNA from the same sample. It saves time and allows us to do multiple analyses with 1 sample. This kit is much simpler and faster than purifying DNA and RNA from Trizol without this kit and I think the columns give you a more pure product than traditional pelleting."

Review date: 05 Jun 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Dept. Neurosciences, Biomedicine and Movement Sciences , University of Verona



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
It simplified my life!
Rating: 4.7

  • Application Area:Isolation of RNA from tissue in Trizol

"I achieved excellent and reproducible results with the use of the Direct-zol RNA Miniprep Kits. My samples were very delicate and "precious" and with the normal RNA extraction techniques I had not obtained sufficient yields for the subsequent analysis (NGS). The extraction protocol is very simple, I didn't need to contact sales assistance and it is convenient as far as value for money."

Review date: 05 Jun 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: HRAE Ciudad Salud



  • Ease of use
    4 out of 5
    After sales service
    3 out of 5
    Value for money
    5 out of 5
Good results, I would use these reagent again.
Rating: 4.0

  • Application Area:RNA extraction in samples of polymorphonuclear cells

"We use this product to facilitate obtaining RNA. It is quite practical and when using, it emits less odors than other similar products."

Review date: 05 Jun 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: University of California, Los Angeles



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Great results! I’ll switch to Direct-zol for all my RNA extractions.
Rating: 5.0

  • Application Area:Extraction of RNA

"Great product! Easy to use and an extremely fast way of extracting RNA. Very reproducible and it avoids messy handling of phenol-based solutions."

Review date: 05 Jun 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Rutgers University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Great RNA quality, superb customer service.
Rating: 5.0

  • Application Area:Isolation of total RNA from E. coli

"The product's protocol is extremely easy to follow and the yield/purity of the RNA is impeccable. I only use this RNA isolation kit for cDNA library construction for Next Generation Sequencing projects. The product, compared to other companies', is fairly priced. Interacting with customer service regarding troubleshooting or questions about the product was one of the best experiences I had. Zymo research's customer service operators go above above and beyond to help resolve the any issues. Honestly, it is worth purchasing any item from Zymo research because of the really amazing customer service. It is absolutely worth spending money with Zymo research because of the company's customer service and high quality of their products. I am very pleased with the products I purchased from Zymo Research and will continue to both purchase more products and recommend the company to others!"

Review date: 05 Jun 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: University of Minnesota



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    4 out of 5
Great results, great cost.
Rating: 4.0

  • Application Area:RNA purification for NGS

"More cost effective than competitors. Nice quality of RNA, I was able to use purified RNA for RNA-Seq. The quality of RNA was perfect."

Review date: 05 Jun 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Yale School Of Medicine



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Awesome product! Methylation studies made easy.
Rating: 5.0

  • Application Area:DNA methylation analysis

"Easy to use. Reproducible results. Value for money and excellent customer care."

Review date: 05 Jun 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2015

  • Organization: Univerisity of Louisville



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
Great results. Easy and saves your time.
Rating: 4.7

  • Application Area:Real-time PCR

"This is a super simple, easy, and highly efficient product. I've never failed since I started using this product. It is just so easy and saves your time. You will always expect a high yield for even a small amount of cells. In most cases, for my flow cytometry sorted cells, I can only have around 0.05 to 0.1 million, but it is enough for me to get a high quality of total RNA. It is fantastic product."

Review date: 05 Jun 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: The University of Michigan



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Fast, easy, highest quality RNA.
Rating: 5.0

  • Application Area:Extract RNA from organoids for qPCR

"This is a lifesaver for me - very easy to use and gives fast results. Most importantly, my RNA is always of the highest quality (based on the RIN using the Agilent 2100). This is one product that I would hate to not have available."

Review date: 05 Jun 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Advanced Reviewer

  • Member since: 2016

  • Organization: Florida State University



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    5 out of 5
Great quality and cost performance.
Rating: 4.3

  • Application Area:Preparing RT-qPCR template

"This product constantly gives high-quality RNA. The small elution volume is also convenient. Since I had no problem, I did not give high score for the after sales care."

Review date: 05 Jun 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: CNRS UMR9004



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Optimal satisfaction.
Rating: 4.3

  • Application Area:RNA modomic on RNA viruses

"Efficient RNA purification. Fast and reliable. No RNA degradation at all."

Review date: 05 Jun 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Bucknell University



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
Excellent products and reliable results.
Rating: 4.7

  • Application Area:RNA extraction from bacteria

"I have always used Zymo products due to their ease of use and high quality results. I work exclusively with undergraduates and the directions are clear and easy to follow. I can even get whole classes to work through the protocols together - a little like herding cats. The students really have to work to mess up the protocols and they rarely do."

Review date: 05 Jun 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Unimedizin Mainz



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
100% satisfied.
Rating: 5.0

  • Application Area:Gene expression of murine lymphocytes

"Easy and fast handling of the samples which ensures no degradation or contamination, high yield of RNA isolated."

Review date: 05 Jun 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Freie Universität Berlin



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Great resuls.
Rating: 5.0

  • Application Area:Analyze gene-operon transcription under oxidative stress in human pathogenic bacteria

"It is easy to use, fast and can obtain high quality RNA."

Review date: 05 Jun 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Max Planck Institute of Immunobiology and Epigenetics



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Reproducible, pure and quick!
Rating: 5.0

  • Application Area:Purification of RNA from tissues and cells (from as small as 17 nucleotides)

"This is a great kit for the isolation of RNA from tissues and cells. I have personally used it for isolation of RNA from drosophila ovaries and drosophila cells. The kit gives pure RNA from these samples. Also useful for isolating small RNA samples as RNA molecules as small as 17 nucleotides can be purified. The amounts of RNA is also reproducibly high. The in-kit DNase treatment also works well to eliminate all DNA."

Review date: 05 Jun 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Ms.



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Great and pure RNA yield.
Rating: 5.0

  • Application Area:Analyse mRNA levels

"Easy to use with multiple samples. It's fast and gives pure and high RNA yield."

Review date: 05 Jun 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: UKM



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
I will definitely use it again.
Rating: 4.7

  • Application Area:Quantitative real time-PCR

"I used this kit for isolation of RNA out of small pieces of mouse aorta. The amount of RNA was okay, the RNA integrity was good (as high as 5). The advantage of this kit is its simplicity and the low loss of RNA on the way due to the direct isolation method. Moreover the kit is cheaper than the ones of several other companies."

Review date: 05 Jun 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: UPF



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Highly recommended.
Rating: 5.0

  • Application Area:E. coli RNA extraction

"It was a pretty easy to use kit. I got really good RNA concentrations."

Review date: 05 Jun 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: University of Heidelberg



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
The best quality of RNA I ever had.
Rating: 4.7

  • Application Area:RNA extraction

"Easy and fast way to extract RNA. Even when I need to prepare samples for further analysis, mine always have the best RIN score. Highly recommended."

Review date: 05 Jun 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Pennsylvania Sate University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Amazing RNA yield and quality. So far, so good. Definitely a favorite one!
Rating: 5.0

  • Application Area:Analyze small RNAs in Corals and their symbiont

"After trying some kits for small RNA extraction, I finally find this one. Working with corals (non-model organism) is not easy, the amount of RNA it is dependent on the amount of calcium skeleton I can get rid of. I usually get a small amount of tissue. With these kit I extracted consistently an amazing RNA yield (~20ug) from 500 ug of coral tissue :D . The quality is great, beautiful peaks in the Bioanalyzer, even though with other kits I always got very small amount and not with this excellent quality. I really like to work fast when extracting RNA. This kit is super easy to work and will save you a ton of time. "

Review date: 29 Jul 2016 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: NCSU Toxicology



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
Great results for the price.......must for our line of work..... Reproducible
Rating: 4.7

  • Application Area:Gene expression by qPCR

"Ready to use kit.... Easy protocol.....RNA yields are of high quality.....genomic DNA residue is minimal to none....would recommend highly"

Review date: 29 Jul 2016 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: UPR-Medical Sciences Campus



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Excellent kit!
Rating: 5.0

  • Application Area:Analyze RNA in blood samples

"The product is very user-friendly and the price is affordable. I really recommend the product and the company. The product was effective for the application and we obtained high quality of RNA. In addition, the kit provided reproducible results in the samples. I’m very happy and satisfied with the product. Many thanks for giving this kind of product to advance scientific projects."

Review date: 29 Jul 2016 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University of Tennessee, Knoxville



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Excellent and efficient way to get RNA.
Rating: 5.0

  • Application Area:Gene expression between control and knockdown in Drosophila male testis lysates

"This is a great product. Saves a lot of time for RNA extraction. Yields high amount of RNA from a less quantity of tissue samples compared to other brands. I loved their free sample program for this kit. I strongly recommend Drosophila scientists to use it for isolating RNA for their qRT-PCR experiments."

Review date: 29 Jul 2016 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: UC Irvine



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    2 out of 5
Good yield, easy to use, fast!
Rating: 3.3

  • Application Area:Analyze the activity of transcription factor/efficiency of gene knockdown in cell cultures

"Kits are very easy to use, require short time to finish the isolation process, result in high yield. The price should be reduced for bulk purchases."

Review date: 29 Jul 2016 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Henry Ford Health System



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    3 out of 5
Very satisfactory.
Rating: 3.7

  • Application Area:Neuroimmunology

"The Direct-zol™ RNA MiniPrep Plus is very easy and time saving with good yields. Thank you Zymo Research for making molecular biology life easy. "

Review date: 28 Jul 2016 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University of Illinois at Urbana-Champaign



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Good consistent results from hard to use tissue
Rating: 5.0

  • Application Area:Extract muscle RNA

"Great product that has clean results with infrequent contamination."

Review date: 21 Jul 2016 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Baylor college of medicine



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    4 out of 5
not bad
Rating: 4.0

  • Application Area:heart and liver of mouse

"Short protocol is a little different from full protocol. The remarks are not obvious. Sometimes it makes me confused. "

Review date: 13 Jul 2016 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Temple University Lewis Katz School of Medicine



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Great results, I will definitely continue to use this kit.
Rating: 5.0

  • Application Area:analyze gene expression from cell samples

"The product was very effective. I was able to isolate clean and quality RNA from primary human cells and reverse transcribe the RNA to cDNA and perform qRT-PCR effectively."

Review date: 12 Jul 2016 | Direct-zol™ RNA Miniprep Plus

The Direct-zol RNA Kits provide a streamlined method for the purification of up to 100 µg (per prep) of high-quality RNA directly from samples in TRI Reagent® or similar.  Total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.).

Isolation of RNA by conventional phase separation was shown to selectively enrich for some species of miRNA, leading to bias in downstream analysis.  The Direct-zol method assures unbiased recovery of small RNAs including miRNA (see below).

The procedure is easy.  Simply apply a prepared sample in TRI Reagent® directly to the Zymo-Spin Column and then bind, wash, and elute the RNA.  No phase separation, precipitation, or post-purification steps are necessary.  The eluted RNA is high quality and suitable for subsequent molecular manipulation and analysis (including RT-PCR, transcription profiling, hybridization, sequencing etc.).

The entire procedure typically takes only 7 minutes.

 

  MiniPrep Plus MiniPrep MicroPrep
RNA Recovery 100 µg 50 µg 10 µg
Minimum Elution 50 µl 25 µl 6 µl
(Animal) Cells ≤ 107 ≤ 5 x 106 ≤ 106
Tissue ≤ 50 mg ≤ 25 mg ≤ 5 mg

*U.S. Patent No. 9,051,563 and other pending patents.

Product Overview

Direct-zol™ RNA Miniprep Plus by Zymo Research product image

Direct-zol™ RNA Miniprep Plus

Manufacturer Zymo Research

4.7 / 5.0 | 71 reviews