Direct-zol™ RNA Miniprep Plus by Zymo Research

"Excellent to purify RNA in an easy and fast way with high quality and quantity RNA."

"The kit has been working very well with consistent results with good RNA quality and yield. The spun columns are handy compared to the Trizol method. The procedure is straightforward to follow as well."

"Very interesting, feasible, and highly efficient method for RNA extraction from adipose tissue."

"RNA quality is very good and the kit is very easy to use, which makes the whole procedure more reproducible even in unexperienced hands. You can quickly get high quality RNA without worries of losing your RNA pellet."

"Direct Zol is a simple and robust kit to get your RNA out of cells. You may better off with your turboDNAse treatment, althugh kit has its own DNAse in it and recomended to use following the protocol."

"This kit is easy to use and we can get enough amount of RNA for RT-qPCR from mouse skeletal muscle tissues and cultured cells. The protocol is simple and we do not need any special equipment for this kit. The price is not as expensive as other similar kits."

"This kit was very useful to extract RNA from cancer cells to use for qPCR experiments. The instructions were easy and efficient!"

"I have used so far 10 Direct-zol RNA Miniprep Kits to purify RNA from adherent and suspension cell samples lysed in Trizol and always performed the optional on-column DNAse digestion. The yields were extremely good, most samples show RNA yield more than expected over OD 260/280 2.0. I stopped using big brand kits, which take a much longer time to purify with lower yields and this Direct-zol RNA Miniprep Kit seemed like such a convenient alternative."

"It is easy to use, reliable, and accurate. Good for beginners as well."

"Easy to use, reproducible results, and no special aftercare needed."

"I find the Direct Zol RNA isolation kit the fastest, easiest, and highest yielding method for isolation of RNA from Trizol extracted Drosophila tissue lysates. So much easier than centrifugation followed by phase separation. I've also started using the kit to clean up capped mRNA T7 ARCA transcription reactions, and the yields are wonderful! Usually get 600 to 700 ng/microliter of clean useable mRNA for insect transgene injections. I highly recommend this product."

"Is really easy to use and reduces steps on the traditional method to obtain pure RNA."

"We’ve been working to generate pipeline with bulk RNA seq. Our lab tried several kits from different companies, then decided to purchase the entire kit from Zymo after trying a sample kit. This kit gave us highest amount of RNAs with hight quality for RNA-seq from both whole tissue and cells collected from FACS. The columns works perfect with Trizol agent-which is the most common reagent and in-column digestion to remove DNAs was another benefit! Easy to follow the directions and reasonable price for 50 preps, compared to other competitors. Highly recommend."

"Direct-zol™ RNA Miniprep Plus is excellent value for money over similar products."

"It was easy to manipulate, product isolated was good on both yield and quality."

"The RNA yield was better than other products, but the column-based method does not have a high throughput. It would be helpful if the product can be improved for automated pipelines."

"Gives you great quality RNA in high concentrations every time! It’s very simple and easy to use and instructions are crystal clear. "

"It is easy to use, you can use trial in the beginning. The protocol is short and effective. Technical support is good."

"This product is quick and easy to use. Very reliable with clean RNA. We switched from a standard RNA isolation method to this product because of its consistency and reliability of the final product."

"This is a great and easy kit that reliably gives really high quality and great yield as well. Our lab's go-to for RNAseq projects."

"This is a very simple and fast product for RNA extraction from a variety of yeast cells. Postgraduate and PhD students routinely isolate mRNA from several yeast species. We tested several species from Pichia, Candida, Saccharomyces, and Komagataella. On average we get RIN value of 9 for the isolated RNA which is great for our downstream applications expression analysis. The recovery is good too, with yield ranging from 850-1000 ng/ul RNA. We simply love the on column DNAase digestion, clean up in 45 minutes. Above all, the price per sample fits to our budget. With wide range of distributors we get this product in the remote area in weeks. It fits the bill perfectly."

"Efficient, high quality easy to use."

"The product is extremely easy to use and saves time compared to the competition, without compromising the yield and quality of the extracted RNA."

"I have been using this product for a year now and it works very good for RNA isolation from all kinds of samples."

"Absolutely easy to use. The students were able to complete the experiment independently and the results were very similar to the results from Qiagen."

"High quality and clean recovery of products, Easy to use, Fast and time saving."

"It is easy to use, I especially love that we do not have to pellet the bacteria and resuspend before lysis. The yield and quality is good as well. Worth the money, and you save time and work."

"I found this kit one of the easiest ones to extract RNA from tissue samples and it works very nicely as well with smear samples (from uterine content). The protocol steps are easy to follow and I have always received the product in great condition - I bought it several times already!"

"Great product."

"Overall, I think this is a great product. It is easy to use and the steps are pretty clear. You can't beat the price either. My only negative comment is that the DNase doesn't seem to work very well. Our labs ended up buying it from another supplier and just using it with this kit."

"The product comes easy to use, with minimal steps after trizol application to 2D tissue culture on plastic or glass. The purity comes out pretty close to what is reported, although it seems less consistent when cell counts are lower. Time of isolation is cut significantly. I think it is worth it if you are willing to pay a premium compared to traditional methods of isolation."

"The procedure is clear, easy to carry out, the amount of mRNA was lower than expected and the quality was regular, we are in the standardization phase, I hope that once the points to be corrected have been identified, the performance of the insert can be achieved."

"We purchace Zymo Resesrch because, the data we get is better in comparison with other companies."

"This product is really easy to use. Take less time and generate high quality RNA in most of my samples."

"This product is 100% effective when used in extracting RNA from cell / tissue samples. The simplicity of the protocol is amazing. It is well detailed and easy to follow. The RNA yield is usually pretty accurate based on my hypothesis. Also, the product content last a long time."

"This kit gives reproducible results all the time I use it and the most important thing is that it is very easy to use just by following the procedure."

"I have used exclusively Zymo products for 10 years now in all my RNA and DNA extractions. Great products, increase reproducibility, easy to follow protocols."

"This is my go-to product for the application. It's easy to use, quick and efficient. The protocol is clear enough that students can handle the extraction process too."

"The product is cheap and easygoing. The results are delightful."

"Good quality RNA can be obtained with easy to use kit."

"The protocol is easy to use and the produced total RNA is high purity and does not contain genomic DNA, since there is a step for DNase incubation during purification. The yield is comparable to other vendors."

"It worked nicely. I got a lot of RNA with good quality. 260/280 is 2.5."

"A very good product, has detailed protocol, easy to use and can get good results. Highly recommend."

"The product was good and RNA quality was good."

"The RNA yields were pretty good, it was easy to use and definitely worth the money."

"I obtained reproducible results and it is easy to use."

"Direct-zol RNA Miniprep Plus kit is the best in the market for RNA extraction. It is easy to use and gives great yield. The best part is that it can also extract small RNAs from samples. I would definitely recommend for use to everyone!"

"Reliable and consistent quality of RNA obtained when using these Zymo kits."

"It’s a very good and reliable product. We use it to extract RNA from fungal samples, and it’s quite easy to use and compatible with trizol stored samples. The only thing is that we tend to have to do a second round of DNAse digestion to remove trace amount of gDNA contamination. The customer service is very nice and once they even offered to fill up our back ordered kit with free samples (didn’t go for that but really appreciate their kind gesture to do anything possible to care for the customers). RNA quality off the column is always good."

"Really ease to use RNA purification kit directly from phenol/trizol or directly extraction from cell. I use in general from trizol (cells ans tissue). It work really nice, with very good RIN number (>9 in general) generated in bioanlayzer equipment. RNA was used to downstream RNAseq analysis, cDNA generation an RT-qPCR without problems."

"Direct-zol™ RNA Miniprep Plus is a very simple and fast resolution of the RNA extraction issues. RIN values usually higher than 9.0 for the fresh tissues."

"Zymo's Direct-zol RNA Miniprep Plus is the bomb! It provides the easiest, best, and most economical way for us to isolate RNA from microbial cells (especially fungi). The on-the-column DNase is quicker and easier to use than other kits where the treatment has to be done separately."

"Used this product for RNA isolation from animal cells. The quality of isolated RNA was very good in terms of RIN value. Used the isolated RNA for cDNA synthesis, gene-expression microarray and transcriptome sequencing."

"This is an easy to use RNA prep kit with good quality."

"Easy protocol, got desirable RNA quality."

"This is a very good product that was recommended by my colleagues. It is suitable for the extraction of yeast/Candida total RNA from samples stored in Trizol. It is very easy to use, takes around 30-40 total to finish the extraction, on column DNase digestion and the final concentration reading. The recovery is good too, I ususally get 800-1000 ng/ul RNA from the column. One thing to notice that is these columns do have maximum capacity. Since we tend to have slightly higher cell numbers to apply to the column, we have used an additional ‘in the tube’ DNase digestion step to make sure the complete removal of gDNA contamination. We also purchase the columns separately just for regular RNA cleaning procedure. All the qRT results look quite good."

"The quality I am looking for in PBMC is usually as high as possible in both ratios 260/280 and 260/230 because these samples are usually sent for sequencing and many of them are nor replaceable. This kit, in addition to be easy to use and to explain to others, ensure high yields and ratios if it is used properly. Highly recommended to use!"

"1. It's easier to use with column filters than regular Trizol extraction of RNA. DNase I included guarantees the quality of RNA without DNA contamination, good for various downstream applications. Also, the small RNA is collected and can be used for more comprehensive RNA profiling. 2. I remember once I contacted the technical support for some questions, I got the reply pretty quick. That makes me more confident in the company's devotion on customers. 3.The price is more competitive compared to other companies, considering the performance and easiness."

"The Direct-zol RNA Miniprep Plus Kit is very effective for purifying total RNA from Human cancer cells. The protocol is pretty straightforward and time efficient. The RNA quality is very high. I used the RNA for the total RNA sequencing. The data is great with average read quality higher than 35.50. And the results are very reproducible. I strongly recommend this product."

"This product yields amazing results with easy to use instructions. It is a time saver - a big plus for the lab. Very cost effective and highly reproducible. High quality RNA. In my lab, students can follow instructions without having prior experience minimizing errors and repeats. Quality of reagents is superb. I would highly recommended."

"This kit significantly reduces the time of extraction without compromizing the quality of RNA. The protocol is very easy to follow and it does not require any special reagent preparation. I strongly recommend it for tissue and cells."

"Its easy to use and provides good quality RNA."

"It is easy to use and saves a lot of time. It also has a lot of DNA contamination. I am not sure if other users have the same problems."

"Easy to use and reproducible if you follow all the steps of their protocol correctly!"

"This kit is more affordable and processes samples faster than those from competitors. Recovery rate of RNA of interest is higher than 90% In our hands. This kit is what we use for all our viral RNA extraction work."

"Easy to use, very user-friendly process."

"It is a really nice product. I was able to isolate viral RNA from the HEK Transfected cells. HEK cells were transfected with pnL4-3 to produce virions."

"The kit is useful when obtaining high quality RNA, but does require longer time usage of DNaseI than mentioned. Overall, it is an efficient kit that yields good product. Additionally, the instructions are clear and straight forward and easy to modify is needed."

"This kit worked great for what I needed! I needed to extract RNA from CVL samples for metatranscriptomics and it was perfect for the job, very easy to use."

"Works well, is fast and easy. No complaints. Yield is sufficiently high for our purposes."

"I had tons of RNA in trizol and was looking for a spin column alternative instead of the usual precipitation. I came across this kit and order a sample kit. This is simple to use without the fear of RNASes. Plus the DNASe is provided as well which is a good touch. I also ended up doing a bioanalyziner and the RIN was always been 8-9 which was brilliant. (Plus one sample was in trizol for more than a year and the RIN was 9). I did some NGS with these samples and was satisfied. Can't complain, thumbs up!"

"Easy to use, good yield and great kit for degraded samples."

"I use this kit to extract RNA from the duodenal and liver tissue of pigs. The protocol is easy to follow and the results are very satisfying. A step by step guide from the manual will help you through and it does not take much time. Good value for money."

"I used this kit with a modified method, still doing phase separation with tr-regent and then putting the aqueous layer through the column in this kit. That worked really well for me and gave consistently good RNA. This modified protocol worked for polysaccaride rich tissues - mytlus mussel species that needed some extra care to extract good RNA."

"The kit combines ease of use and speed together with the ability to isolate high concentrations of high quality RNA, with little to no contaminating DNA that could interfere with downstream applications such as RT-PCR and microarray analysis. It worked well for both Gram-negative bacteria (E. coli and S. typhimurium) and hard to lyse mycobacterial species, such as M. tuberculosis."

"Super easy. I haven't benchmarked it against other kits, but happy with the output."

"Great, fast and similar quantity and quality of isolated nucleic acid, good performance in cDNA synthesis."

"Easy to use. High-quality results and reproducible. Will recommend this product."

"The Zymo support team was very helpful in assisting us to find the right product for our needs. They gave us free samples, and checked in to make sure they were the right fit for our use."

"This product is time saving and easy to use. It gives good purity and all kinds of RNA, which is good for RNA research."

"This kit is easy to use and results in high quality RNA. We get consistent results using it for downstream RT-qPCR. We also have achieved good results with RNA-seq."

"This kit is amazing, but it has one technical issue. I used it for placental samples dissolved in RNAzol. The high fat of the samples make the column completely hydrophobic so water would pass after a spin down, but no RNA would be eluted. Outside of this instance, I've never had an issue."

"Product is extremely easy to use for degradation-sensitive material, is much less risky for exposure and contamination of phenol-related waste compared to traditional RNA extraction methods. Easy for undergraduates to use. High yield of RNA, high quality and works well for RT-qPCR directly."

"I got good quality results by using this kit. It was excellent for restriction digestion."

"Easy to use, high production, a good product worth buying."

"The kit is easy to use, the extracted RNA is of high quantity and quality. It's especially useful that Zymo provides kit versions with columns that have different RNA capacities and enable also smaller elution volumes."

"I used this product to extract RNA for transcriptomics with two bacterial species. It was an excellent product! It was easy to use, the protocol was straightforward and clear, and we got excellent quality RNA! It fared better than similar products that we have tried in the past. We were very impressed with the product!"

"This kit is really easy to use. It's also quite fast! I like that it allows you to use samples already prepared in TRIzol. So I can homogenize my samples in TRIzol and store them at -80C until I am ready to proceed with RNA extraction. The downside is that in like 50% of time (10 out of 20 samples) I got a very bad 260/230 ratio (0.94-1.5) so I had to do additional RNA purification step. In short, I would say, yes, I recommend it if you are ready to perform an additional RNA precipitation or column clean-up step."

"Directzol provides high amount of RNA within a short time and the quality is good and reproducible."

"I love Zymo RNA purification kits because they are easy to use and give reproducible data every time I use them."

"Very easy to use and the results are reproducible and stable."

"The RNA prep kit is excellent for yielding clean RNA in a user-friendly way. The steps are simple and easy to follow and were easily adapted with the RNA isolation protocol that was already in place. This kit is also great for RNA isolation from tissues; once the tissue is homogenized in the Trizol reagent, it can be spun to separate the aqueous phase before just following the rest of the Zymo protocol. The RNA is very clean every time and I am able to use a lower concentration of RNA for each PCR reaction!"

"The RNA Miniprep delivers high quality and reproducible total RNA, which is ideal for short-read NGS applications. The recovery was high and the protocol was simple, rapid, and easy to follow"

"Direct-zol was a windfall for my research project! After spending months generating stable mutants, I then had to analyze target protein levels with and without chemokine treatment. As my P.I. usually prefers the "old-world" method and anything found Maniatis et al, I was originally directed to use a more common tri-reagent. However, the results were not reproducible. After spending weeks convincing the P.I. to get the Direct-zol kit, they relented, and it was so worth it. It is so much easier than handling large lysates, and reproducible every 3 times! The after sales care is pretty simple, just follow the reagent storage conditions. The bang for your buck is stellar because you get the answer you need and CAN MOVE ON! The only thing I can mention is that it may be worth eluting in two steps (no additional volume needed, just use 1/2 volume RNAse free water and soak column for 1 minute before spin-down, then repeat allowing 1-minute soak.)"