Direct-zol™ RNA Miniprep Plus by Zymo Research

"You get clean, intact RNA with the kit that is the highest quality for downstream applications."

"Faster yet costlier RNA from trizolized samples. small starting sample does not result in higher yield than using the traditional trizol/Phenol chloroform methods."

"It is very easy to use, the results were of high quality, and reproducible, although concentration was a bit low. I have not required any after-sales care. I only checked a free sample."

"Easy to use and cost-effective. Would recommend for reasonably low throughput."

"Zymo Direct-zol has been one of the most easy to use and robust kits for RNA isolation for me. I have used it for RNA-seq as well routine qPCR experiments and have been satisfied with it's performance over the years."

"This kit is super easy to use and is very convenient. Before this, we were using the trizol-chloroform method, which was messy and took a lot of time for the number of samples we usually prepare."

"Always reliable results and fast and easy procedures"

"Easy to use, large quantity of RNA, however DNA elimination is not complete. Bashing beads are necessary to complete lysis of cell walls."

"We have been using the Direct-zol™ RNA Miniprep Plus kit for a couple of years now. The cuticle of nematodes is a little difficult to deal with sometimes, but we have had consistent quality of RNA from the kit. DNA is even easier."

"It is easy to use and a very fast procedure. In addition to that, it results in great yield and quality."

"It's very easy to use, time-saving and has fewer steps. Will definitely buy again."

"It works well for tissue only but it’s not easily adapted for other sample types. I work with a variety of sample types and would like a kit that’s easily adaptable to the sample type I’m working with."

"This product is single tube and uses a bench-top centrifuge for a very quick and easy extraction process. We have been able to achieve a decent yield and reproducible quality from this kit, on a variety of starting sample sizes and from both neurons and astrocytes."

"The product is easy to use and we obtained a very good amount of RNA from human blood. It's also not expensive."

"We were able to obtain a free kit to test and were able to retrieve high yields of RNA from in-vitro transcription."

"It was easy to use, worked well, came out with good results, and is even cheaper than some competitors."

"Easy of use. Manufacturer recommendations are clear enough. Safety data sheet OK."

"The RNA kit is easy to handle and fast to use. Otherwise we only use Trizol, but this time we switched to a kit from Zymo Research. The RNA yield was very good, the quality too. Inhibitors from the sample matrix were not observed. The cDNA synthesis proceeded without complications. We recommend this kit."

"It was very helpful because I was able to extract RNA with a very good quality in a very short time."

"This kit was purchased for RNA extraction from plant material for work on a low titre virus. RNA was used for PCR and we were able to get consistent and repeatable results."

"We got a very good quality of RNA from limited amount of specific area in cortex. The price of the kit is very good for research experiments. Moreover, the protocol is much easier to carry out and saves a lot of time. It does not require the centrifuge to be cooled to 4 degrees any more, which is very convenient for most labs. Our students like the kit very much compared to the previous classical one. We would like to order more in future."

"We have been using Trizol following the manufacturer indications to extract RNA from tissue and cell lines. However, we wanted to improve the extraction in terms of purity while reducing the hands on time. We found out about Direct-Zol and tested it. Our RNA improved in quality and we reduced the time for extraction. In addition there's no need for chloroform addition, which is outstanding."

"Direct-zol has been very effective to isolate total RNA highly purified to use as a template to the synthesis of cDNA. For purposes to amplified samples by qPCR, high quality samples of RNA are obtained easily and quickly. The value for money is very good, the time required, the quantity of sample and the easy way to perform the procedure justify the cost."

"This is the first time I've used this new RNA extraction kit, so I had a lot of questions to ask. The customer service did a great job answering my questions. The steps of this protocol were quite easy to follow and I got good quality RNA."

"Consistent data and easy to use. Had no problem in following the protocol."

"I am extremely satisfied using the Direct-zol RNA MiniPrep Plus kit! I no longer have to worry about the days of using the dreaded chloroform/Trizol extraction method which would sometimes take hours to finish. The Direct-zol protocol is easy, efficient, and can be finished in less than 30 minutes! Now I can extract RNA and run a qPCR for the same samples all within the same day! I love using the Direct-zol kit and can't imagine doing RNA extraction without it!"

"It is fast but the quality is not that great. I found some smears after use."

"Good quality with a reasonable price compared to competitors' products. The results are comparable, but the price is significantly lower."

"Super easy to use! RNA was clean upon extraction for our downstream assays."

"The product was easy to use and gave me good quality RNA. It was good value and delivered quickly."

"We think the protocol that comes with the kit is very easy to read and follow, even first year undergraduate researchers can easily get familiar with the procedures, which increases the lab's efficiency greatly in terms of cutting down the training time. Also it always yields good RNA concentration, for both cells and animal tissues. We deal with mouse colon tissue a lot and due to the small portion we have, we really appreciate this product and its high yield rate with good quality control. Ever since we started to use this product, we have conducted several animal studies and published multiple manuscripts with it, and we will continue using it in future."

"As a developmental biologist in a mostly undergraduate university, I need a product that delivers high-quality RNA in a format that is highly reproducible and cost-effective. My undergrads find the protocol easy to follow as it has far fewer steps than competitors' products. Students' results are more consistent between experiments because of the simpler format. The end product is of high quality and we have used the RNA for qPCR and NGS experiments successfully. My ratings are all five star. The kit is much cheaper than those of other major competitors and after sales service is excellent."

"It's a very simple and straightforward kit to use. We used it to extract RNA from sorghum leaf and internode tissue which is very hardy but this kit made it easy to extract good quality RNA isolation for qRT PCR and RNA Seq applications."

"This is a great product. Design of column prevents alcohol or trizol contamination in final RNA. Quality of RNA is great and never seen any DNA in RNA prep. This product is good for small RNA also. It gives reproducible result."

"This kit has not only saved us money compared to other similar kits (which are usually hundreds of dollars more) but is also both easy to use and produces great results."

"RIN =10 for all RNA preparations, good yield of miRNAs, results are highly reproducible, kit is easy to use. It is compatible with any lysis buffer. The cost is much better or comparable with similar products from other vendors. However it's performance is much better. Technical support is readily available but I really never needed it as the kit works without failure exactly as is suggested by the manual."

"Reproducibly high quality. Very easy to use. Easy to scale up too."

"Generally it's good. The miniprep steps saved us much time. The extracted product has high purity but low yield."

"Hight quality DNA/RNA purification. Provides good results for epigenetics."

"The kit is easy to use and the isolated RNA is of high quantity and quality. Important, by using these columns you don't lose small RNAs."

"Easy to use, quicker protocol than most other vendors, great, reproducible results!"

"This kit was very easy to use. I received high quality and reproducible results. Worked for exactly what I needed."

"I had problems getting good quality RNA in NK cells and found an easy and clean way to use trizol extraction."

"The kit was very easy and a welcome change from acidic phenol. I was surprised how good the yield and quality of the RNA was. The RNA was great and I obtained the reverse transcribed DNA the first time and it was easy to subclone."

"Achieve excellent results, easy to use, cost effective."

"This kit helps me transfer from traditional trizol purification steps with simple column loading and washing. It is cool! I do have a suggestion: is there any possibility to omit the extra DNase digestion step and provide a more efficient DNA elimination column before RNA purification column?"

"Very easy to use. Zymo makes great kits. Very affordable pricing."

"Works great even for the most degraded samples. Easy to use and very fast."

"This is a great easy to use kit to purify high quality DNA and RNA from the same sample. It saves time and allows us to do multiple analyses with 1 sample. This kit is much simpler and faster than purifying DNA and RNA from Trizol without this kit and I think the columns give you a more pure product than traditional pelleting."

"I achieved excellent and reproducible results with the use of the Direct-zol RNA Miniprep Kits. My samples were very delicate and "precious" and with the normal RNA extraction techniques I had not obtained sufficient yields for the subsequent analysis (NGS). The extraction protocol is very simple, I didn't need to contact sales assistance and it is convenient as far as value for money."

"We use this product to facilitate obtaining RNA. It is quite practical and when using, it emits less odors than other similar products."

"Great product! Easy to use and an extremely fast way of extracting RNA. Very reproducible and it avoids messy handling of phenol-based solutions."

"The product's protocol is extremely easy to follow and the yield/purity of the RNA is impeccable. I only use this RNA isolation kit for cDNA library construction for Next Generation Sequencing projects. The product, compared to other companies', is fairly priced. Interacting with customer service regarding troubleshooting or questions about the product was one of the best experiences I had. Zymo research's customer service operators go above above and beyond to help resolve the any issues. Honestly, it is worth purchasing any item from Zymo research because of the really amazing customer service. It is absolutely worth spending money with Zymo research because of the company's customer service and high quality of their products. I am very pleased with the products I purchased from Zymo Research and will continue to both purchase more products and recommend the company to others!"

"More cost effective than competitors. Nice quality of RNA, I was able to use purified RNA for RNA-Seq. The quality of RNA was perfect."

"Easy to use. Reproducible results. Value for money and excellent customer care."

"This is a super simple, easy, and highly efficient product. I've never failed since I started using this product. It is just so easy and saves your time. You will always expect a high yield for even a small amount of cells. In most cases, for my flow cytometry sorted cells, I can only have around 0.05 to 0.1 million, but it is enough for me to get a high quality of total RNA. It is fantastic product."

"This is a lifesaver for me - very easy to use and gives fast results. Most importantly, my RNA is always of the highest quality (based on the RIN using the Agilent 2100). This is one product that I would hate to not have available."

"This product constantly gives high-quality RNA. The small elution volume is also convenient. Since I had no problem, I did not give high score for the after sales care."

"Efficient RNA purification. Fast and reliable. No RNA degradation at all."

"I have always used Zymo products due to their ease of use and high quality results. I work exclusively with undergraduates and the directions are clear and easy to follow. I can even get whole classes to work through the protocols together - a little like herding cats. The students really have to work to mess up the protocols and they rarely do."

"Easy and fast handling of the samples which ensures no degradation or contamination, high yield of RNA isolated."

"It is easy to use, fast and can obtain high quality RNA."

"This is a great kit for the isolation of RNA from tissues and cells. I have personally used it for isolation of RNA from drosophila ovaries and drosophila cells. The kit gives pure RNA from these samples. Also useful for isolating small RNA samples as RNA molecules as small as 17 nucleotides can be purified. The amounts of RNA is also reproducibly high. The in-kit DNase treatment also works well to eliminate all DNA."

"Easy to use with multiple samples. It's fast and gives pure and high RNA yield."

"I used this kit for isolation of RNA out of small pieces of mouse aorta. The amount of RNA was okay, the RNA integrity was good (as high as 5). The advantage of this kit is its simplicity and the low loss of RNA on the way due to the direct isolation method. Moreover the kit is cheaper than the ones of several other companies."

"It was a pretty easy to use kit. I got really good RNA concentrations."

"Easy and fast way to extract RNA. Even when I need to prepare samples for further analysis, mine always have the best RIN score. Highly recommended."

"After trying some kits for small RNA extraction, I finally find this one. Working with corals (non-model organism) is not easy, the amount of RNA it is dependent on the amount of calcium skeleton I can get rid of. I usually get a small amount of tissue. With these kit I extracted consistently an amazing RNA yield (~20ug) from 500 ug of coral tissue :D . The quality is great, beautiful peaks in the Bioanalyzer, even though with other kits I always got very small amount and not with this excellent quality. I really like to work fast when extracting RNA. This kit is super easy to work and will save you a ton of time. "

"Ready to use kit.... Easy protocol.....RNA yields are of high quality.....genomic DNA residue is minimal to none....would recommend highly"

"The product is very user-friendly and the price is affordable. I really recommend the product and the company. The product was effective for the application and we obtained high quality of RNA. In addition, the kit provided reproducible results in the samples. I’m very happy and satisfied with the product. Many thanks for giving this kind of product to advance scientific projects."

"This is a great product. Saves a lot of time for RNA extraction. Yields high amount of RNA from a less quantity of tissue samples compared to other brands. I loved their free sample program for this kit. I strongly recommend Drosophila scientists to use it for isolating RNA for their qRT-PCR experiments."

"Kits are very easy to use, require short time to finish the isolation process, result in high yield. The price should be reduced for bulk purchases."

"The Direct-zol™ RNA MiniPrep Plus is very easy and time saving with good yields. Thank you Zymo Research for making molecular biology life easy. "

"Great product that has clean results with infrequent contamination."

"Short protocol is a little different from full protocol. The remarks are not obvious. Sometimes it makes me confused. "

"The product was very effective. I was able to isolate clean and quality RNA from primary human cells and reverse transcribe the RNA to cDNA and perform qRT-PCR effectively."