Direct-zol™ RNA Miniprep Plus by Zymo Research

"It is easy to use and saves a lot of time. It also has a lot of DNA contamination. I am not sure if other users have the same problems."

"Easy to use and reproducible if you follow all the steps of their protocol correctly!"

"This kit is more affordable and processes samples faster than those from competitors. Recovery rate of RNA of interest is higher than 90% In our hands. This kit is what we use for all our viral RNA extraction work."

"Easy to use, very user-friendly process."

"It is a really nice product. I was able to isolate viral RNA from the HEK Transfected cells. HEK cells were transfected with pnL4-3 to produce virions."

"The kit is useful when obtaining high quality RNA, but does require longer time usage of DNaseI than mentioned. Overall, it is an efficient kit that yields good product. Additionally, the instructions are clear and straight forward and easy to modify is needed."

"This kit worked great for what I needed! I needed to extract RNA from CVL samples for metatranscriptomics and it was perfect for the job, very easy to use."

"Works well, is fast and easy. No complaints. Yield is sufficiently high for our purposes."

"I had tons of RNA in trizol and was looking for a spin column alternative instead of the usual precipitation. I came across this kit and order a sample kit. This is simple to use without the fear of RNASes. Plus the DNASe is provided as well which is a good touch. I also ended up doing a bioanalyziner and the RIN was always been 8-9 which was brilliant. (Plus one sample was in trizol for more than a year and the RIN was 9). I did some NGS with these samples and was satisfied. Can't complain, thumbs up!"

"Easy to use, good yield and great kit for degraded samples."

"I use this kit to extract RNA from the duodenal and liver tissue of pigs. The protocol is easy to follow and the results are very satisfying. A step by step guide from the manual will help you through and it does not take much time. Good value for money."

"I used this kit with a modified method, still doing phase separation with tr-regent and then putting the aqueous layer through the column in this kit. That worked really well for me and gave consistently good RNA. This modified protocol worked for polysaccaride rich tissues - mytlus mussel species that needed some extra care to extract good RNA."

"The kit combines ease of use and speed together with the ability to isolate high concentrations of high quality RNA, with little to no contaminating DNA that could interfere with downstream applications such as RT-PCR and microarray analysis. It worked well for both Gram-negative bacteria (E. coli and S. typhimurium) and hard to lyse mycobacterial species, such as M. tuberculosis."

"Super easy. I haven't benchmarked it against other kits, but happy with the output."

"Great, fast and similar quantity and quality of isolated nucleic acid, good performance in cDNA synthesis."

"Easy to use. High-quality results and reproducible. Will recommend this product."

"The Zymo support team was very helpful in assisting us to find the right product for our needs. They gave us free samples, and checked in to make sure they were the right fit for our use."

"This product is time saving and easy to use. It gives good purity and all kinds of RNA, which is good for RNA research."

"This kit is easy to use and results in high quality RNA. We get consistent results using it for downstream RT-qPCR. We also have achieved good results with RNA-seq."

"This kit is amazing, but it has one technical issue. I used it for placental samples dissolved in RNAzol. The high fat of the samples make the column completely hydrophobic so water would pass after a spin down, but no RNA would be eluted. Outside of this instance, I've never had an issue."

"Product is extremely easy to use for degradation-sensitive material, is much less risky for exposure and contamination of phenol-related waste compared to traditional RNA extraction methods. Easy for undergraduates to use. High yield of RNA, high quality and works well for RT-qPCR directly."

"I got good quality results by using this kit. It was excellent for restriction digestion."

"Easy to use, high production, a good product worth buying."

"The kit is easy to use, the extracted RNA is of high quantity and quality. It's especially useful that Zymo provides kit versions with columns that have different RNA capacities and enable also smaller elution volumes."

"I used this product to extract RNA for transcriptomics with two bacterial species. It was an excellent product! It was easy to use, the protocol was straightforward and clear, and we got excellent quality RNA! It fared better than similar products that we have tried in the past. We were very impressed with the product!"

"This kit is really easy to use. It's also quite fast! I like that it allows you to use samples already prepared in TRIzol. So I can homogenize my samples in TRIzol and store them at -80C until I am ready to proceed with RNA extraction. The downside is that in like 50% of time (10 out of 20 samples) I got a very bad 260/230 ratio (0.94-1.5) so I had to do additional RNA purification step. In short, I would say, yes, I recommend it if you are ready to perform an additional RNA precipitation or column clean-up step."

"Directzol provides high amount of RNA within a short time and the quality is good and reproducible."

"I love Zymo RNA purification kits because they are easy to use and give reproducible data every time I use them."

"Very easy to use and the results are reproducible and stable."

"The RNA prep kit is excellent for yielding clean RNA in a user-friendly way. The steps are simple and easy to follow and were easily adapted with the RNA isolation protocol that was already in place. This kit is also great for RNA isolation from tissues; once the tissue is homogenized in the Trizol reagent, it can be spun to separate the aqueous phase before just following the rest of the Zymo protocol. The RNA is very clean every time and I am able to use a lower concentration of RNA for each PCR reaction!"

"The RNA Miniprep delivers high quality and reproducible total RNA, which is ideal for short-read NGS applications. The recovery was high and the protocol was simple, rapid, and easy to follow"

"Direct-zol was a windfall for my research project! After spending months generating stable mutants, I then had to analyze target protein levels with and without chemokine treatment. As my P.I. usually prefers the "old-world" method and anything found Maniatis et al, I was originally directed to use a more common tri-reagent. However, the results were not reproducible. After spending weeks convincing the P.I. to get the Direct-zol kit, they relented, and it was so worth it. It is so much easier than handling large lysates, and reproducible every 3 times! The after sales care is pretty simple, just follow the reagent storage conditions. The bang for your buck is stellar because you get the answer you need and CAN MOVE ON! The only thing I can mention is that it may be worth eluting in two steps (no additional volume needed, just use 1/2 volume RNAse free water and soak column for 1 minute before spin-down, then repeat allowing 1-minute soak.)"

"This kit works amazingly with cell culture samples; however, RNA extracted from placental samples did not work. I think it was the high fat content. This made it so the elution buffer or water would not absorb into the column at the final stage. The water simply beaded on top of the column and still passed through upon centrifugation, but no RNA was eluted. This has only happened for placental samples in my hands and forces me to use phase separation. There was no indication this could happen in the manual."

"Easy kit to use, detailed information, and great results. Recommended."

"High quality RNA was extracted using the Zymo kit. It was very easy, toxic free, and efficient."

"I used this product during grad school and it works well. I also liked the service and the value we got for the product. I would order and use this again and I will recommend it to colleagues."

"I like this kit, it is easy to use and very fast."

"Very easy to use and gives great results. Make sure you use really good sterile water in your sample prep for best quality RNA."

"Great product! Quick & easy extraction of high quality RNA from primary patient cells!"

"During my ten years of experience in the lab, I have tried all gel purification kits in the market (seriously)! From the cheaper to the most expensive ones, the easy to the complicated ones, non-branded to the well-known brands. However, I can summarize that the easiest, highest-yielding, quickest, and most affordable kit is definitely by Zymo Research, and every time I recommended it to other labs."

"We've used this kit for RNA prep from Arabidopsis and rice samples that have been homogenized in Trizol. Compared to the conventional method that involves chloroform and ethanol precipitation, this kit has been much easier to use and has helped us get better reproducible results."

"The kit is very easy to use and results have been reproducible. To get higher yield, the additional optional step prior to cell lysis was helpful."

"It is straightforward to use, but I didn't get useful extraction indexes. My sample was contaminated, and I had to try some extra steps."

"It produces high quality total RNA. We use the RNA to do RT-PCR. The RNA is very pure and the results are very reproducible. It is also easy to use, we can run 50 samples in 2 hours. Another important thing is the price, it is much cheaper than other similar kits."

"It was a good poduct to use. I had used kits from QIAGEN, Machery Nagel and Invitrogen for RNA isolation and I found it quite competitive with respect to other kits. The quality and quantity of the isolated RNA was satisfactory."

"We have received great results. It is easy and fast to isolate RNA from the samples. We are very impressed by the results."

"I used a sample kit from the miniprep and I purchased the kit immediately. I have been using this kit for a year and I don’t plan to look for something else. The kit is easy to use and the purity is high. Customer service is very quick in responding to any question and are very helpful."

"It is very effective and easy to use. Very valuable! High RNA extraction efficiency."

"You get clean, intact RNA with the kit that is the highest quality for downstream applications."

"Faster yet costlier RNA from trizolized samples. small starting sample does not result in higher yield than using the traditional trizol/Phenol chloroform methods."

"It is very easy to use, the results were of high quality, and reproducible, although concentration was a bit low. I have not required any after-sales care. I only checked a free sample."

"Easy to use and cost-effective. Would recommend for reasonably low throughput."

"Zymo Direct-zol has been one of the most easy to use and robust kits for RNA isolation for me. I have used it for RNA-seq as well routine qPCR experiments and have been satisfied with it's performance over the years."

"This kit is super easy to use and is very convenient. Before this, we were using the trizol-chloroform method, which was messy and took a lot of time for the number of samples we usually prepare."

"Always reliable results and fast and easy procedures"

"Easy to use, large quantity of RNA, however DNA elimination is not complete. Bashing beads are necessary to complete lysis of cell walls."

"We have been using the Direct-zol™ RNA Miniprep Plus kit for a couple of years now. The cuticle of nematodes is a little difficult to deal with sometimes, but we have had consistent quality of RNA from the kit. DNA is even easier."

"It is easy to use and a very fast procedure. In addition to that, it results in great yield and quality."

"It's very easy to use, time-saving and has fewer steps. Will definitely buy again."

"It works well for tissue only but it’s not easily adapted for other sample types. I work with a variety of sample types and would like a kit that’s easily adaptable to the sample type I’m working with."

"This product is single tube and uses a bench-top centrifuge for a very quick and easy extraction process. We have been able to achieve a decent yield and reproducible quality from this kit, on a variety of starting sample sizes and from both neurons and astrocytes."

"The product is easy to use and we obtained a very good amount of RNA from human blood. It's also not expensive."

"We were able to obtain a free kit to test and were able to retrieve high yields of RNA from in-vitro transcription."

"It was easy to use, worked well, came out with good results, and is even cheaper than some competitors."

"Easy of use. Manufacturer recommendations are clear enough. Safety data sheet OK."

"The RNA kit is easy to handle and fast to use. Otherwise we only use Trizol, but this time we switched to a kit from Zymo Research. The RNA yield was very good, the quality too. Inhibitors from the sample matrix were not observed. The cDNA synthesis proceeded without complications. We recommend this kit."

"It was very helpful because I was able to extract RNA with a very good quality in a very short time."

"This kit was purchased for RNA extraction from plant material for work on a low titre virus. RNA was used for PCR and we were able to get consistent and repeatable results."

"We got a very good quality of RNA from limited amount of specific area in cortex. The price of the kit is very good for research experiments. Moreover, the protocol is much easier to carry out and saves a lot of time. It does not require the centrifuge to be cooled to 4 degrees any more, which is very convenient for most labs. Our students like the kit very much compared to the previous classical one. We would like to order more in future."

"We have been using Trizol following the manufacturer indications to extract RNA from tissue and cell lines. However, we wanted to improve the extraction in terms of purity while reducing the hands on time. We found out about Direct-Zol and tested it. Our RNA improved in quality and we reduced the time for extraction. In addition there's no need for chloroform addition, which is outstanding."

"Direct-zol has been very effective to isolate total RNA highly purified to use as a template to the synthesis of cDNA. For purposes to amplified samples by qPCR, high quality samples of RNA are obtained easily and quickly. The value for money is very good, the time required, the quantity of sample and the easy way to perform the procedure justify the cost."

"This is the first time I've used this new RNA extraction kit, so I had a lot of questions to ask. The customer service did a great job answering my questions. The steps of this protocol were quite easy to follow and I got good quality RNA."

"Consistent data and easy to use. Had no problem in following the protocol."

"I am extremely satisfied using the Direct-zol RNA MiniPrep Plus kit! I no longer have to worry about the days of using the dreaded chloroform/Trizol extraction method which would sometimes take hours to finish. The Direct-zol protocol is easy, efficient, and can be finished in less than 30 minutes! Now I can extract RNA and run a qPCR for the same samples all within the same day! I love using the Direct-zol kit and can't imagine doing RNA extraction without it!"

"It is fast but the quality is not that great. I found some smears after use."

"Good quality with a reasonable price compared to competitors' products. The results are comparable, but the price is significantly lower."

"Super easy to use! RNA was clean upon extraction for our downstream assays."

"The product was easy to use and gave me good quality RNA. It was good value and delivered quickly."

"We think the protocol that comes with the kit is very easy to read and follow, even first year undergraduate researchers can easily get familiar with the procedures, which increases the lab's efficiency greatly in terms of cutting down the training time. Also it always yields good RNA concentration, for both cells and animal tissues. We deal with mouse colon tissue a lot and due to the small portion we have, we really appreciate this product and its high yield rate with good quality control. Ever since we started to use this product, we have conducted several animal studies and published multiple manuscripts with it, and we will continue using it in future."

"As a developmental biologist in a mostly undergraduate university, I need a product that delivers high-quality RNA in a format that is highly reproducible and cost-effective. My undergrads find the protocol easy to follow as it has far fewer steps than competitors' products. Students' results are more consistent between experiments because of the simpler format. The end product is of high quality and we have used the RNA for qPCR and NGS experiments successfully. My ratings are all five star. The kit is much cheaper than those of other major competitors and after sales service is excellent."

"It's a very simple and straightforward kit to use. We used it to extract RNA from sorghum leaf and internode tissue which is very hardy but this kit made it easy to extract good quality RNA isolation for qRT PCR and RNA Seq applications."

"This is a great product. Design of column prevents alcohol or trizol contamination in final RNA. Quality of RNA is great and never seen any DNA in RNA prep. This product is good for small RNA also. It gives reproducible result."

"This kit has not only saved us money compared to other similar kits (which are usually hundreds of dollars more) but is also both easy to use and produces great results."

"RIN =10 for all RNA preparations, good yield of miRNAs, results are highly reproducible, kit is easy to use. It is compatible with any lysis buffer. The cost is much better or comparable with similar products from other vendors. However it's performance is much better. Technical support is readily available but I really never needed it as the kit works without failure exactly as is suggested by the manual."

"Reproducibly high quality. Very easy to use. Easy to scale up too."

"Generally it's good. The miniprep steps saved us much time. The extracted product has high purity but low yield."

"Hight quality DNA/RNA purification. Provides good results for epigenetics."

"The kit is easy to use and the isolated RNA is of high quantity and quality. Important, by using these columns you don't lose small RNAs."

"Easy to use, quicker protocol than most other vendors, great, reproducible results!"

"This kit was very easy to use. I received high quality and reproducible results. Worked for exactly what I needed."

"I had problems getting good quality RNA in NK cells and found an easy and clean way to use trizol extraction."

"The kit was very easy and a welcome change from acidic phenol. I was surprised how good the yield and quality of the RNA was. The RNA was great and I obtained the reverse transcribed DNA the first time and it was easy to subclone."

"Achieve excellent results, easy to use, cost effective."

"This kit helps me transfer from traditional trizol purification steps with simple column loading and washing. It is cool! I do have a suggestion: is there any possibility to omit the extra DNase digestion step and provide a more efficient DNA elimination column before RNA purification column?"