CyTRAK Orange
An orange probe staining both nucleus and cytoplasm.
Live cell segmentation
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Very useful stain to interpret your microscopy data.
Visualize nuclei and cell cytoplasm by fluorescent confocal microscopy
Easy to use stain, relatively inexpensive, good stain to localize intracellular organelles and nuclei.
Review Date: 9 Sept 2021 | Biostatus Limited
Nice results.
2-photon live-imaging
We used CyTRAK Orange to label nuclei of acute brain slices and performed 2-Photon live-imaging. The labeling is really easy and fast to process. This dye is very efficient to label nuclei in thick tissue (300 µm) and did not show any toxic effect.
Review Date: 18 May 2021 | Biostatus Limited
Manufacturer's Response
Dear Christelle, thank you for this review of CyTRAK Orange! We believe this is the first example of its use in 2-photon imaging and it was impressive to see detection through the full depth of 300 micron brain slices.
A fluorescent dye staining both nucleus and cytoplasm. It is water soluble and membrane permeant and can be used in LIVE cells in combination with other common fluors, especially GFP fusions, FITC-labelled antibodies and far-red dyes.
CyTRAK Orange™ can be used as a LIVE cell nuclear stain or as a cell location dye to define the perimeter of the cell in a variety of cell-based assays. It is highly compatible with existing protocols and can be used across a wide range of instrumentation platforms.
- Rapid staining of dsDNA/nuclei of LIVE or fixed cells
- Easy to use - no lyse, no wash, no RNase needed
- Ideal for use with GFP & FITC labels - fluoresces at Emλmax 615 nm
- Ideal for use as a cell location dye in HTS and HCS assays
- Photochemically and biologically very stable
CyTRAK Orange in Live End-Point Imaging
In fluorescence-based microscopy, it is normal to preserve cells or tissue sections with formaldehyde or a similar fixative. However, there are certain problems associated with fixing, such as leakage, and suitability for studying drug-receptor interactions in cells, or other live-cell end-point assays. In this application note, it is shown how cell-impermeant DNA dyes are unusable as nuclear counterstains in such assays, but the orange fluorescing live-cell-permeant DNA probe CyTRAK orange provides clear nuclear counterstaining as a feasible alternative.
Orange Fluorescent Live-Cell Permanent DNA and Cytoplasmic Dye
This app note describes the use of CyTRAK Orange™ in hyperphosphatemic familial tumoral calcinosis (HFTC) screening. CyTRAK is a permanent dsDNA- binding probe that allows for live-cell labeling and is used to highlight, and consequently separate, nucleated cells using flow cytometry. It overcomes many of the traditional issues of nucleated cell differentiation, such as lengthy process time, release of debris and inconsistent results.
Phenotypic Screening and In Vitro Toxicology: Applications of Far‐Red Fluorescing DNA-Specific Anthraquinones in Cell‐Based Assays
This application note demonstrates a number of applications of Fluorescein-/GFP-compatible dyes, in particular DRAQ5 and CyTRAK Orange. The particular spectral and chemical features of DRAQ5 and CyTRAK Orange mean they are compatible with aqueous media and fixatives. Conveniently, they are supplied in aqueous solution and can be applied as the final step in a sample preparation. They have become standard reagents for immunofluorescence microscopy, flow cytometry and high content imaging.
