Member since: 2019
Organization: Institute of Hepatology
Perfect for organoid cultures.
Application Area: Organoids culture
"This is a perfect alternative to Matrigel from Corning. I have not noticed a difference in the performance and I recommend it considering the price advantage."
Cultrex UltiMatrix RGF BME is an optimized and effective growth scaffold for organoid, pluripotent stem cell, and advanced 3D culture applications. It can be used undiluted or diluted to a specific protein concentration.
Why use Cultrex UltiMatrix RGF BME for Organoid and Pluripotent Stem Cell Culture?
Cultrex UltiMatrix Reduced Growth Factor (RGF) Basement Membrane Extract (BME) is a soluble form of basement membrane that provides high tensile strength, enhanced levels of entactin/nidogen, elevated protein concentration, and robust clarity and purity that ensures that the hydrogel contains the maximal amount of soluble extracellular matrix proteins. These compositional and concentration enhancements make Cultrex UltiMatrix RGF BME the choice matrix with proven efficacy in organoid cell culture, induced pluripotent stem cell expansion and differentiation, spheroid formation, and other 2D and 3D culture applications.
Cultrex UltiMatrix RGF BME is purified from mouse Engelbreth-Holm-Swarm (EHS) tumor, Cultrex UltiMatrix RGF BME is viscous at 2-8 °C and gels at 37 °C to form a reconstituted basement membrane.
How Do I Use Cultrex UltiMatrix RGF BME?
Basement membrane hydrogels play an essential role in developing complex in vitro and ex vivo cell and tissue culture models. By providing an optimized mixture of laminin, collagen IV, entactin/nidogen, and heparan sulfate proteoglycans, hydrogels like Cultrex UltiMatrix RGF BME provide native cell-matrix interactions that influence cell behavior and native developmental programs that promote complex cell culture and 3D tissue generation in vitro.
To preserve its performance benefits, Cultrex UltiMatrix RGF BME should be thawed overnight at 2-8 °C; the thawed hydrogel solidifies quickly at temperatures above 15 °C. When thawing, aliquoting, and diluting Cultrex UltiMatrix RGF BME, keep it on ice to prevent untimely gelling.