Nuclease-independent applications of CRISPR provide equal targeting specificity but instead of cutting, CRISPRi allows for targeted interference of gene function by delivering transcriptional repressor domains to a specific target sequence using modified dCas9 + gRNA complexes. Gene knockdown is complementary to CRISPR-KO and CRISPRa (activation) and has distinct advantages over existing loss-of-function strategies like RNAi.
Merck partnered with University of California San Francisco to provide the best-in-class CRISPRi screening tools available. Superior algorithm placement and gRNA scaffold improvements enhance your CRISPRi screening experiments.
Each pool contains one subpool of top 5 gRNAs per gene as well as a separate subpool of 5 supplemental gRNAs per gene for increased sensitivity and includes non-targeting controls
We are a 10x Genomics compatible partner allowing downstream single-cell analysis simple with built in feature barcode technology if requested
Vectors contain both BFP and Puromycin as selection markers and an optimized scaffold for sgRNA expression and function
KRAB-dCas9 gene silencing allows loss-of-function screening with less risk of toxicity to your cells due to double stranded breaks
Individual CRISPRi clones can be easily ordered online or by contacting your local sales representative. Custom pools are also available