Reagent kit that enables rapid lysis of yeast cells for highly efficient protein extraction and simultaneous protection of extracted proteins against a multitude of proteases
Application
Use Complete Lysis-Y to rapidly lyse cells of various yeast strains (e.g., Saccharomyces cerevisiae, Schizosaccharomyces pombe, Pichia pastoris) in only 20 minutes at room temperature and simultaneously inhibit protease activity in the cell lysate. The kit enables highly efficient protein extraction and concurrent inhibition of a multitude of proteases, including serine proteases, cysteine proteases, and metalloproteases.
Benefits
Product Description
Due to the complex and stable cell wall, yeast protein extraction is difficult and time consuming. The Complete Lysis-Y yeast-cell protein extraction reagent contains a mild ready-to-use solution that efficiently lyses the following organisms: Saccharomyces cerevisiae, Schizosaccharomyces pombe, and Bacillus subtilis, eliminating the need for glass beads. Rapid cell lysis occurs in just 20 minutes at room temperature. The protein yields obtained with this kit are two times higher compared to those obtained by using glass beads.
In combination with Complete, Mini Protease Inhibitor Cocktail Tablets, included in the kit, the Lysis-Y Reagent provides a very convenient and efficient way to extract proteins and simultaneously inhibit protease activity during the initial extraction steps.
Complete Tablets eliminate the time-consuming search for the right protease inhibitor. The ready-to-use water-soluble, non-toxic tablets work optimally with the kit’s Lysis-Y Reagent. The extracted proteins can be further purified or analyzed in downstream applications.
Contents
200 ml Lysis-Y Reagent for protein extraction from yeast cells
20 Complete, Mini Protease Inhibitor Cocktail Tablets supplied in EASYpacks (each tablet is sufficient for 10 ml of extract)
Manufacturer Roche Applied Science - a member of the Roche Group
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