Member since: 2016
Organization: M.S. University of Baroda, vadodara
Very effective. Tissue digestion is easy and faster.
Application Area: Cancer stem cells culture
"It is good value-of-money and made my research easy."
Collagenase from Clostridium histolyticum has been used:
Collagenase has been used in the preparation of arterial tissue for the study of Advanced Glycosylation End Products (AGE). The enzyme has also been used along with other proteases for the disaggregation of human tumor, mouse kidney, human brain, lung epithelium and many other tissues. It is also effective in liver and kidney perfusion studies, digestion of pancreas, and isolation of nonparenchymal hepatocytes. This enzyme is tested for suitability for the release of hepatocytes, at approx. 1 mg/mL conc., in a total volume of 100 mL for each rat liver.