CellTag™ 700 Stain In-Cell Western™ Kits
The CellTag™ 700 Stain ICW (In-Cell Western™) Kits provide detection reagents for cell-based In-Cell Western Assays.
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The CellTag™ 700 Stain ICW (In-Cell Western™) Kits provide detection reagents for cell-based In-Cell Western Assays.
Each kit includes Intercept (PBS) Blocking Buffer, IRDye® 800CW secondary antibody for detection of a specific protein target in the 800 nm channel, and CellTag™ 700 Stain to normalize well-to-well variations in cell number. This cost-effective normalization method makes quantification of the target protein more precise.
CellTag 700 Stain is a near-infrared fluorescent, non-specific cell stain that provides accurate normalization to cell number for In-Cell Western applications. The stain accumulates in both the nucleus and cytoplasm of permeabilized cells, and provides linear fluorescent signal across a wide range of cell types and cell numbers. CellTag 700 Stain is applied to the cells during incubation with IRDye® 800CW secondary antibody, and enables accurate measurement of target protein levels with much higher throughput than Western blotting.
Correlation of Complex Signaling Pathway Interactions with Cell Migration in a Combined Cell Migration-Phosphorylation Assay
This scientific poster from LI-COR Biosciences presents a screening assay that allows simultaneous assessment of cell migration and kinase activity. It is shown that the Oris Cell Migration Assay platform can be successfully used in combination with LI-COR Odyssey and Aerius instruments to obtain multiplexed data on the inhibition of cell migration by different classes of compounds, as well as their effects on intracellular signaling pathway events using the In-Cell Western technique. A better understanding of the mechanisms underlying cell movement and metastasis may facilitate a more efficient means of developing anti-cancer drugs.
Using In-Cell Western Assays to Study Alzheimer's Disease
Watch the video, to learn how the In-Cell Western Assay Kits from LI-COR have been used with the Odyssey® Imaging system to study Alzheimer's Disease. The presentation demonstrates how the In-Cell Western Assay Kits are used for screening compounds that increase Hsp90 inhibitors expression, decrease tau expression and are able to cross the blood-brain barrier (BBB).
Rethinking Research - How to Use the In-Cell Western™ Assay
Watch this fascinating educational webinar from LI-COR featuring the review of several examples of published research data, in which the In-Cell Western assay was used. Find out how this assay can broaden the scope of scientific research.
An Update on Alzheimer’s Disease Research
Discover SelectScience® video interviews and articles about the latest Alzheimer’s disease research methods
