Member since: 2020
Organization: Regional Ayurveda Research Institute(under CCRAS )
Cost-effective and precise analytical tool for natural products.
Application Area: Natural product chemistry
"The HPTLC system is so effective for the purpose of identification and quantification of natural drugs. Sampling is cost-effective, precise, and reproducible. Multiple samples can be analyzed simultaneously."
Automatic sample application is a key factor for productivity of the HPTLC laboratory. The ATS 4 offers fully automatic sample application for qualitative and quantitative analyses as well as for preparative separations. It is suited for routine use and high sample throughput.
Samples are either applied as spots through contact transfer (0.1–5 μL) or as bands or rectangles (0.5 to > 50 μL) using the spray-on technique. Starting zones in the form of narrow bands offer the best separation attainable with a given chromatographic system. Application in the form of rectangles allows precise application of large volumes without damaging the layer. Prior to chromatography, these rectangles are focused into narrow bands with a solvent of high elution strength.
The ATS 4 allows “overspotting”, i.e. a sequential application from different vials onto the same position. This technique can be used e.g. in pre-chromatographic derivatization, spiking, etc.