Status:
Reviewer
Member since: 2019
Organization: HUJI
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- Ease of use
- After sales service
- Value for money
Reproducible results
Application Area: Separate biologics products coupled to mouse IgG subunits.
"We had reproducible results and easy handling with this column."
Review date: 17 Jan 2019 | BioResolve Columns
Status:
Reviewer Member since: 2013
Organization: Pfizer
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- Ease of use
- After sales service
- Value for money
The BioResolve RP Column is superior to any RP column on the market.
Application Area:Chromatography separation
"The BioResolve RP Column is superior to any RP column on the market. I have gotten excellent results for separations by LC/MS. This column will be our next platform column. It has superior resolution, recovery, and separation."
Review date: 20 Dec 2018 | BioResolve Columns
Status:
Reviewer Member since: 2018
Organization: sanofi pasteur
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- Ease of use
- After sales service
- Value for money
Shorter analysis times, better resolution
Application Area:Vaccines and proteins
"High quality and reproducible. Easy to use, great application support team at Waters."
Review date: 18 Oct 2018 | BioResolve Columns
Status:
Reviewer Member since: 2018
Organization: EPFL
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- Ease of use
- After sales service
- Value for money
Good product!
Application Area:LCMS analysis of IgG
"Very good separation of mouse IgG subunits. Need conditioning with intact IgG prior to use to keep nice separation properties."
Review date: 09 Oct 2018 | BioResolve Columns
Status:
Reviewer Member since: 2018
Organization: Ipsen Bioinnovation
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- Ease of use
- After sales service
- Value for money
Greatly improved carryover!
Application Area:MS Analysis of Biotherapeutic
"The column - we have a particularly hydrophobic biotherapeutic. Using the traditional C4 column for RP-UPLC and RP-MS methods I always have to run at least three injections of MP A until the carryover has been cleared. With the BioResolve column, this has been reduced to one MP A injection. I have not experienced the added resolution as the analytes are fairly well resolved anyway. Waters - These guys are second to none when it comes to customer service. I've not had any need to troubleshoot any methods with the column so the after sales care has been limited to follow up calls to see how we're getting on with the product, etc. I have previously used their Chemistry Customer Service Team for another product where they significantly helped me with method dev/troubleshooting."
Review date: 09 Oct 2018 | BioResolve Columns
Status:
Reviewer Member since: 2018
Organization: Celonic Deutschland GmbH Co. KG
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- Ease of use
- After sales service
- Value for money
Highly recommend this column
Application Area:Impurities
"We had reproducible results and easy handling with this column. No or negligible sample carry-over occurred. We can highly recommend this column and will use it as soon as possible for other projects. We achieved very good results compared to the data of a column we previously used (another manufacturer). We gained much more resolution between the impurities. Before this column, we had a lot of problems with carry-over."
Review date: 08 Oct 2018 | BioResolve Columns
Status:
Reviewer Member since: 2018
Organization: Takeda
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- Ease of use
- After sales service
- Value for money
Good value
Application Area:Biologics characterization
"More choice to separate biologics products coupled to LC-MS, except for SEC and C4"
Review date: 05 Oct 2018 | BioResolve Columns
Status:
Reviewer Member since: 2018
Organization: IECB
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- Ease of use
- After sales service
- Value for money
Great results even with complicated samples.
Application Area:Nanobodies
"We purchased this column to characterize our nanobodies which have a molecular weight of around 14 KDa and it worked really well. Even without expecting it when analyzing them by UPLC-MS with the BioResolve column we were able to distinguish two separate peaks corresponding to the wild type nanobody and an N-terminal pyroglutamat form of it which only differs on 17 units of mass. With that we can say that this column has a really good resolution and is able to distinguish between two close species which may be really useful when working with antibody's modifications."
Review date: 05 Oct 2018 | BioResolve Columns
Status:
Reviewer Member since: 2018
Organization: Pfizer
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- Ease of use
- After sales service
- Value for money
The resolution and recovery of subunits analysis met our expectations.
Application Area:Analyzed mAb for the modification of subunits
"The resolution of BioResolve column is much better than C4 columns. We achieved reproducible results for subunits analysis when using HPLC and UPLC. The assay robustness is fine with three different lots of columns. Comparing to the similar columns, the cost of column is reasonable."
Review date: 05 Oct 2018 | BioResolve Columns
Status:
Reviewer Member since: 2018
Organization: Pfizer
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- Ease of use
- After sales service
- Value for money
Slightly better than typical RP column, needs adjusted parameters for proteins
Application Area:RP assay for biopharmaceutical protein candidate
"We evaluated the BioResolve column on one protein using a reduced / alkylated (subunit-like) method. Slightly better peak resolution was observed on highly glycosylated protein, and needed to lower flow rate and column temperature."
Review date: 03 Oct 2018 | BioResolve Columns
Status:
Reviewer Member since: 2018
Organization: MedImmune
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- Ease of use
- After sales service
- Value for money
Great product, helpful with every day chromatography.
Application Area:Determination of drug to antibody ratio of specified molecules
"We are able to achieve high quality, reproducible results using these analytical columns. The data obtained from the individual experiments assists us in moving forward with other analytical processes."
Review date: 03 Oct 2018 | BioResolve Columns
Status:
Reviewer Member since: 2018
Organization: Dept Clin Biochem, Vejle Hospital, Denmark
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- Ease of use
- After sales service
- Value for money
Promising results.
Application Area:analysis of therapeutic antibody
"We have only used the column for a few injections until now but it is better than just running the proteins on a C18 column."
Review date: 02 Oct 2018 | BioResolve Columns
Status:
Reviewer Member since: 2018
Organization: KBI Biopharma
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- Ease of use
- After sales service
- Value for money
Great product, unfortunate molecule problems prevent my use!
Application Area:Biotherapeutic method development (reverse phase chromatography)
"After initial column screening for my method development, I found that the BioResolve column had the best peak shape, resolution, and reproduciblity for my molecule. The only factor that played into me not choosing this column was back pressure buildup, as my molecule was very sensitive to acidic conditions and likely was precipitating on the column. For normal development, I will attempt to use BioResolve again in the future."
Review date: 02 Oct 2018 | BioResolve Columns
Status:
Reviewer Member since: 2018
Organization: Grifols Diagnostics Solutions
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- Ease of use
- After sales service
- Value for money
Worth the money for good separation.
Application Area:mAb Conjugation Determination
"This column is easy to install and easy to use. It provides better resolution then the standard C4 column that we have been using. The instructions for recommended flow rates and gradients were very useful in standard mAb separation and characterization."
Review date: 02 Oct 2018 | BioResolve Columns
Status:
Reviewer Member since: 2018
Organization: Medimmune
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- Ease of use
- After sales service
- Value for money
Resolving power of this column is unmatched by any other C8 or C18 column.
Application Area:Drug to antibody ratio analysis for antibody drug conjugates and free drug analysis for antibody dru
"This column provided very good resolution of the drug conjugated heavy chain and light chain species when reduced and analyzed using RPHPLC. It also resolved fragments and other drug degradants when samples were subjected to different stress conditions. This column was also able to resolve the Cysteinylated variants in the intact monoclonal antibody."
Review date: 26 Sep 2018 | BioResolve Columns
Protein Reversed-Phase Columns
Reversed-phase chromatography of proteins, performed on columns packed with porous or coated, solid-core particles possessing wide pore size particles ( e.g., 300Å), and functionalized with short ligand length chemistries (e.g., C4), is a separation technique based on the ability to separate samples based on relative hydrophobic differences of the proteins in solution. Gradients of increasing organic solvent concentration are frequently used to affect separations in the presence of ion-pairing reagents (e.g., 0.1% TFA or 0.1% formic acid) that minimize undesired ionic interactions. In general, the hydrophobicity of the protein or protein subunit determines the elution order, with the least hydrophobic proteins eluting first. Factors such as particle composition, pore size, ligand type and density, as well as separation conditions (e.g., gradient duration, separation temperature, flow rate) all play important roles in obtaining a separation that meets application requirements.
BioResolve RP mAb Polyphenyl Columns
These 450Å, 2.7 µm columns were developed in response to shortcomings of existing reversed-phase columns designed for LC or LC-MS analysis of intact mAb or mAb subunits.
- Contain silica-based, solid core particles with defined 450Å pore coating for outstanding protein component resolution, recovery, and low injection-to-injection carryover
- Use innovative polyphenyl ligand and bonding technology (patent pending) to deliver superior intact mAb and subunits separations in LC (0.1% TFA) or LC-MS (0.02% TFA or 0.1% FA) applications
- Deliver near equivalent performance on HPLC, UHPLC, and UPLC instrumentation
- QC tested with a mAb subunit standard (i.e., Reduced IdeS-digested NIST mAb Reference Material 8671) to help ensure batch-to-batch column consistency
Product Overview
