Member since: 1989
Great and reliable.
Application Area: Flow cytometry analysis
"I really enjoy using it. Numerous innovations make the system easy to use, powerful, and productive. A fixed alignment flow cell, for example, minimizes startup time and improves reproducibility. And a patented optical design maximizes signal detection and increases sensitivity and resolution for each color in a multicolor assay."
Member since: 2014
Organization: Center of Immunology
A very good product, very helpful for my daily research
Application Area:Analyse protein expression, immuno phenotype, signal transduction, cell cycle
"It is a very good product and stable. I have good and reproducible results during the years - I used it daily. Easy to use and a very good service team. It's not cheap but it's worth the money!"
Member since: 2012
Organization: National Cancer Institute
"We purchased the Facs Canto II with High Through put attachment (HTS). The HTS broke down several times within the first year, they kept giving us refurbished HTSs that were just as bad as the first one. We wanted to purchase a spare HTS that we could swap ourselves because we were tired of losing experiments when it broke down. The company's response? Charge us $8,100 dollars every year for the privilege of having a subpar replacement in a service contract, in addition to our regularly overpriced service contract for the whole machine. Next time we are definitely not purchasing from BD, we are going to the competitors!"
Member since: 2011
Organization: Lehigh University
Application Area:Genome regulation
"Easy to use and overall good value for money. The carousel system facilitates multiple samples. Updated software would be good and after sales service could improve."
Member since: 2011
Organization: BRI, Bristol
"Quick daily maintenance, end of day clean is simple and allows you to walk away. High throughput analyzer. Engineers are very helpful on the phone and very quick to respond to initial contact with technical support. Engineer in lab within 1-2 days. FACSDIVA software allows detailed analysis of data."
Cell analyzer with proven reliability
The BD FACSCanto™ family of benchtop analyzers features reliable performance and accurate results for experiments requiring up to 10 parameters.
Numerous innovations make the system easy to use, powerful, and productive. A fixed alignment flow cell, for example, minimizes startup time and improves reproducibility. And a patented optical design maximizes signal detection and increases sensitivity and resolution for each color in a multicolor assay.
High Performance, Innovative System
The BD FACSCanto™ II system features many innovations. At the heart of the cytometer, for example, the fluidics system features a fixed-alignment flow cell to minimize startup time and improve reproducibility. The BD FACSCanto II fluidics system is designed to streamline work, save time, and improve performance. In the fluidics system, the sample travels up the sample injection tube, and hydrodynamic focusing within the flow cell forces particles into a single-file stream where laser light intercepts the stream at the sample interrogation point. The unique flow cell design permits particles to flow through the center of the flow cell. Increasing the sample pressure increases the core diameter and the flow rate.
A fluidics cart holds large fluid tanks necessary to operate and maintain the instrument. For sample acquisition, positive-pressure pumps in the fluidics cart send sheath fluid past a 0.2-μm filter to a pressurized interior reservoir inside the instrument called the plenum. The plenum maintains a nearly constant fluid level and dampens pump pulsation using a new dynamic feedback pressure control system designed to regulate pressure. As a result, sheath flow rate does not vary with the level of fluid in the sheath cubitainer, and the reservoir automatically removes small air bubbles from the sheath supply.
A high flow rate is generally used for measurements such as immunophenotyping, for which data is less resolved but is acquired more quickly. A lower flow rate is generally used in applications for which optimal resolution and sensitivity are critical.
Daily routine procedures such as startup, shutdown, and cleaning routine cycles are automated as a result of fluidic integration with BD FACSCanto clinical software or BD FACSDiva™ software. BD FACS™ shutdown solution prevents salt crystal buildup in fluidics lines and is supplemented with a preservative to prevent bacterial growth. During the instrument shutdown procedure, BD FACS shutdown solution replaces sheath fluid in all sample and sheath fluid lines.
LEDs are located in the front door to monitor each acquisition parameter. Each LED blinks when the signal level reaches a prescribed threshold.