The BaseScope™ Assay is a unique product from ACD, based on the same platform of proven and established RNAscope® technology that has been cited in over 2300 publications. This powerful assay provides new data dimensions and unique insight into biological mechanisms. The BaseScope™ Assay enables applications such as the detection of exon junctions/splice variants, short/highly homologous RNA sequences (50-300 bases), and point mutations at single cell sensitivity. The BaseScope Singleplex asay is also available on automated plaforms.
The BaseScope™ Reagent Kit v2-RED, is based on ACD’s patented signal amplification and background suppression technology. The assay uses a novel and proprietary method of in situ hybridization (ISH) to visualize single RNA molecules per cell in a multitude of sample types mounted on slides. The BaseScope™ Assay is used for the detection of short RNA targets (50-300 bases), highly homologous sequences, RNA mutations, and exon junctions for the detection of splice variants, circular RNA, and gene fusion. The BaseScope™ Assays do not require an RNase-free environment that is used for traditional RNA ISH. The BaseScope™ Assay leverages the same platform as proven and established within the RNAscope® technology but expands the detection capabilities and throughputness on automated platforms such as Leica Bond RX and Ventana Discovery Ultra.