Automated Droplet Generator (186-4101) by Bio-Rad

Manufacturer Bio-Rad  |   Model: 186-4101  |  Available Worldwide

AutoDG™ Instrument for automated droplet generation of up to 96 samples, for use with the QX200™/QX100™ Droplet Digital™ PCR Systems. The AutoDG Instrument partitions each sample in a 96-well PCR plate into ~20,000 uniform nanoliter-sized droplets in which nucleic acid molecules are randomly distributed. Every droplet serves as an individual PCR reaction in Bio-Rad’s Droplet Digital (ddPCR™) PCR System. After partitioning, the plate is sealed with the PX1™ Plate Sealer, and PCR is run to end po... Read more


Automated Droplet Generator (186-4101) by Bio-Rad product image
Automated Droplet Generator (186-4101)
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AutoDG™ Instrument for automated droplet generation of up to 96 samples, for use with the QX200™/QX100™ Droplet Digital™ PCR Systems.

The AutoDG Instrument partitions each sample in a 96-well PCR plate into ~20,000 uniform nanoliter-sized droplets in which nucleic acid molecules are randomly distributed. Every droplet serves as an individual PCR reaction in Bio-Rad’s Droplet Digital (ddPCR™) PCR System. After partitioning, the plate is sealed with the PX1™ Plate Sealer, and PCR is run to end point in a thermal cycler. The droplets in each sample can then be analyzed using a QX100 or QX200 Droplet Reader.

The AutoDG Instrument eliminates user-to-user variability, allowing you to fully exploit the precision of ddPCR technology by providing more consistent droplet counts across users and plates. The AutoDG Instrument also minimizes hands-on time — it takes less than 5 minutes to load the instrument, and no supervision or user activity is required during droplet generation.


Features:

  • Guides setup and loading with large, color touch-screen interface and LED lighting
  • Generates droplets for 96 ddPCR reactions in under 45 minutes
  • One AutoDG Instrument can generate sufficient droplets to keep 4–5 droplet readers running continuously
  • Provides operational flexibility:
    - User-definable plate layout with increments of 8 wells
    - HEPA-filtered enclosure reduces the possibility of contamination and allows the system to be placed in any lab environment
    - Compatible with both QX100 and QX200 ddPCR workflows

Applications:

  • Cancer biomarker studies and copy number variation
  • Pathogen detection
  • Next generation sequencing
  • Gene expression analysis
  • Environmental monitoring
  • Food testing