ApopTag® Fluorescein In Situ Apoptosis Detection Kit by Merck
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Expert Reviewer
Member since: 2015
Organization: Postdoctoral Associate in Cambridge MA
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- Ease of use
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- Value for money
Great TUNEL staining
Application Area: TUNEL Assay live NP
"This kit provides all the necessary means to perform a TUNEL assay. We utilize this on fixed tissue and it works exceptionally well."
Review date: 15 Nov 2017 | ApopTag® Fluorescein In Situ Apoptosis Detection Kit
The ApopTag® Fluorescein In Situ Apoptosis Detection Kit detects apoptotic cells in situ by the indirect TUNEL method, utilizing an anti-digoxigenin antibody that is conjugated to a Fluorescein reporter molecule. It provides indirect immunofluorescence staining for 40 samples. Results are analyzed using either flow cytometry or fluorescence microscopy.
The ApopTag® Fluorescein In Situ Apoptosis Detection Kit has been tested for specific staining in these model systems: (a) human normal peripheral blood lymphocytes induced with dexamethasone as stained in cytospins, (b) rat regressing mammary gland as stained in formalin-fixed, paraffin-embedded sections, and (c) human leukemic peripheral blood lymphocytes induced with camptothecin, as stained in cell suspensions and used for quantitative flow cytometry.
Components:
- Equilibration Buffer 90416 3.0 mL -15°C to -25°C
- Reaction Buffer 90417 2.0 mL -15°C to -25°C
- TdT Enzyme 90418 0.64 mL -15°C to -25°C
- Stop/Wash Buffer 90419 20 mL -15°C to -25°C
- Blocking Solution 90425 2.6 mL -15°C to -25°C
- Anti-Digoxigenin-Fluorescein 90426 2.1 mL 2°C to 8°C
- Plastic Coverslips 90421 100 ea. Room Temp.
- affinity purified sheep polyclonal antibody
