ApopTag® Fluorescein In Situ Apoptosis Detection Kit by The Life Science business of Merck KGaA, Darmstadt, Germany, operates as MilliporeSigma in the U.S. and Canada

4.3
/
5.0
  |  1 reviews
Detects apoptotic cells in situ by the indirect TUNEL method, utilizing an anti-digoxigenin antibody that's conjugated to a Fluorescein reporter molecule


ApopTag® Fluorescein In Situ Apoptosis Detection Kit by The Life Science business of Merck KGaA, Darmstadt, Germany, operates as MilliporeSigma in the U.S. and Canada product image
ApopTag® Fluorescein In Situ Apoptosis Detection Kit
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Average Rating: 4.3
1 Scientist has reviewed this product

5 out of 5
Ease of use
4 out of 5
After sales service
4 out of 5
Value for money


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  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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Great TUNEL staining
Rating: 4.3

  • Application Area: TUNEL Assay live NP

"This kit provides all the necessary means to perform a TUNEL assay. We utilize this on fixed tissue and it works exceptionally well."

Review date: 15 Nov 2017 | ApopTag® Fluorescein In Situ Apoptosis Detection Kit

The ApopTag® Fluorescein In Situ Apoptosis Detection Kit detects apoptotic cells in situ by the indirect TUNEL method, utilizing an anti-digoxigenin antibody that is conjugated to a Fluorescein reporter molecule. It provides indirect immunofluorescence staining for 40 samples. Results are analyzed using either flow cytometry or fluorescence microscopy.

The ApopTag® Fluorescein In Situ Apoptosis Detection Kit has been tested for specific staining in these model systems: (a) human normal peripheral blood lymphocytes induced with dexamethasone as stained in cytospins, (b) rat regressing mammary gland as stained in formalin-fixed, paraffin-embedded sections, and (c) human leukemic peripheral blood lymphocytes induced with camptothecin, as stained in cell suspensions and used for quantitative flow cytometry.

Components:

  • Equilibration Buffer 90416 3.0 mL -15°C to -25°C
  • Reaction Buffer 90417 2.0 mL -15°C to -25°C
  • TdT Enzyme 90418 0.64 mL -15°C to -25°C
  • Stop/Wash Buffer 90419 20 mL -15°C to -25°C
  • Blocking Solution 90425 2.6 mL -15°C to -25°C
  • Anti-Digoxigenin-Fluorescein 90426 2.1 mL 2°C to 8°C
  • Plastic Coverslips 90421 100 ea. Room Temp.
  • affinity purified sheep polyclonal antibody

Product Overview

ApopTag® Fluorescein In Situ Apoptosis Detection Kit by The Life Science business of Merck KGaA, Darmstadt, Germany, operates as MilliporeSigma in the U.S. and Canada product image

ApopTag® Fluorescein In Situ Apoptosis Detection Kit

Manufacturer The Life Science business of Merck KGaA, Darmstadt, Germany, operates as MilliporeSigma in the U.S. and Canada  |  Available Worldwide

4.3 / 5.0 | 1 reviews