Member since: 2017
Application Area: Analysed RNA-DNA hybrids on telomeres
"We tested the S9.6 Abs in two applications: dot-blot and immunofluorescence staining, including a combination with telomere FISH. We used all the required control and were very happy with the quality and specificity of these Abs. No signal with ssDNA, ssRNA, DsRNA or dsRNA. We also verified elimination of signal by RNAse H. Very clean data. Works perfectly."
Member since: 2015
Organization: Technion Faculty of medicine
Application Area:Immunoprecipitation of DNA:RNA hybrids
"The product arrived in good shape. We aliquoted it and kept at -20 degrees. The antibody worked well and gave expected results on positive and negative controls."
This mouse monoclonal antibody was generated against a ΦX174 bacteriophage-derived synthetic DNA–RNA antigen and recognizes RNA-DNA hybrids of various lengths.
DNA-RNA hybrids are a natural occurrence within eukaryotic cells, with levels of these hybrids increasing at sites with high transcriptional activity, such as during transcription initiation, repression, and elongation. Because RNA-DNA hybrids influence genomic instability, the S9.6 antibody is a useful reagent to help study the consequences of R-loops and lesions formed by these hybrids during DNA replication or other cellular processes. In addition, the S9.6 antibody is effective in recognizing RNA-DNA hybridization for microarray studies.