Automated nanoscale solution for RNA-seq libraries
RNA sequencing (RNA-seq) is the gold standard for hypothesis-free profiling of the transcriptome and is an essential tool for molecular biology laboratories. To keep pace with increasing sample demand, automated microfluidics-based library prep can advance transcriptomics by streamlining laboratory methods and substantially reducing associated costs.
Designed to drive significant improvement in the RNA-seq workflow, the Advanta™ RNA-Seq NGS Library Prep Kit together with the Juno™ system delivers an integrated solution for automated, cost-efficient NGS library prep. The Juno system is easy to install and operate and, with the Advanta RNA-Seq reagents and 48.Atlas™ integrated fluidic circuit (IFC), supports simultaneous processing of up to 48 total RNA samples from eukaryotic organisms, generating libraries compatible with Illumina® sequencing instruments.
The method generates full-length, stranded libraries from random priming of polyadenylated [poly(A)] RNA present in total RNA samples. The Advanta RNA-Seq Kit and Juno workflow improve
upon alternative RNA-seq library prep workflows by providing true walkaway automation while reducing reagent, consumables, labor and instrument costs.