5-mC DNA ELISA Kit by Zymo Research

Manufacturer Zymo Research  |   Model: D5325
4.5
/
5.0
  |  2 reviews
The 5-mC DNA ELISA Kit features a streamlined workflow for high-throughput detection of global 5-methylcytosine (5-mC) in DNA.


5-mC DNA ELISA Kit by Zymo Research product image
5-mC DNA ELISA Kit
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Average Rating: 4.5
2 Scientists have reviewed this product

5 out of 5
Ease of use
5 out of 5
After sales service
4 out of 5
Value for money


  • Status:

    Reviewer
  • Member since: 2016

  • Organization: MIDDLE EAST TECHNICAL UNIVERSITY



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    3 out of 5
Easy to use kit, with low sensitivity and nice price.
Rating: 4.3

  • Application Area: Global DNA methylation in bacteria

"It is a user friendly kit to detect global methylation level. However, the sensitivity was low especially for bacteria. The standards worked well but my samples showed zero methylation which is against the nature :) When I used another kit (different manufacturer) I have obtained reasonable results confirming the literature. I liked the good correlation and low error bars between the replicates of standards, which is serious problem in other kits. To summarize, it should be improved a lot to increase the reliability. The price is reasonable compared with the other manufacturer’s methylation kits."

Review date: 28 Jul 2016 | 5-mC DNA ELISA Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: McGill University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Streamlined workflow
Rating: 4.7

  • Application Area:cytosine modifications

"This product is efficient in estimating global levels of 5mC in DNA from multiple tissue types. The workflow is straightforward and can be completely in several hours."

Review date: 12 Jul 2016 | 5-mC DNA ELISA Kit

The ability to efficiently detect and quantify DNA methylation (i.e., 5-methylcytosine) has become essential for epigenetic-based research.  To date, a number of methods have been developed for this purpose including high-performance capillary electrophoresis, bisulfite sequencing, and methylated DNA immunoprecipitation.

The 5-mC DNA ELISA Kit is a convenient and powerful tool that allows the researcher to accurately quantitate 5-mC in any DNA sample in less than 3 hours. The kit features a unique Anti-5-Methylcytosine monoclonal antibody that is both sensitive and specific for 5-mC.  The assay is compatible with a wide range of input DNA from vertebrate, plant, and microbial sources as well as PCR amplicons and fragmented DNA. Percent 5-mC in a DNA sample can be accurately quantified from a standard curve generated with specially designed controls included with the kit.  Also, the fast, streamlined workflow is ideal for high-throughput analyses.

Product Overview

5-mC DNA ELISA Kit by Zymo Research product image

5-mC DNA ELISA Kit

Manufacturer Zymo Research

4.5 / 5.0 | 2 reviews