Status:
ReviewerMember since: 2022
Organization: CINVESTAV
Great results
Application Area: Analyze fish liver DNA methylation
"It is a very effective and sensitive kit with high quality results and very easy to use. The incubation temperature is very important. It most be at the indicated temperature for optimal results. The antibodies has to be treated with care to maintain the optimal conditions."
Status:
ReviewerMember since: 2022
Organization: CINVESTAV
Excellent results for epigenetics research
Application Area:DNA methylation in fish
"The 5-mC DNA ELISA Kit is a very effective and sensitive DNA methylation kit that contributes to the epigenetic research for non-model species that don’t have a sequenced genome. The results show high quality, and the kit is easy to use. The incubation periods and the temperature in each step are very important to get reliable results. Value for money rating: In Latin America it is usually more expensive due to taxes and acquisition costs."
Status:
ReviewerMember since: 2022
Organization: Frontiers in Bioscience
Basically great
Application Area:5mC DNA methylation ELIZA
"5mC DNA methylation kit is easy to carry out, relatively quick, and nice cost performance. The only thing would be: can't stop the reaction. Maybe it is great if color development can be stopped by adding acid solution (just like other ELISA kits)."
Status:
ReviewerMember since: 2017
Organization: Rutgers New Jersey Medical School
One solution in Epigenetic study
Application Area:Epigenetic modulations
"I purchased a P5mC DNA ELISA kit for assessing DNA methylation in the hippocampus of the rat brain, and I found its works very nice, easy to do this experiment as the user manual is well elaborative and self-explanatory. Results are highly reproducible and their interpretation is also very easy. Once I stuck with standard graph preparation, therefore I wrote to the customer care executive and he responded very well and assisted me to resolve my problem. I am highly satisfied with this product and suggest to all my friends to must consider it while planning to study epigenetic modulations. Its value of money and its timely delivery support me to submit my work on time."
Status:
ReviewerMember since: 2021
Organization: National University of Colombia
Great results!
Application Area:5-mC quantification
"Very easy to use and reproducible results. Very glad with the general functioning."
Status:
ReviewerMember since: 2021
Organization: Frontiers in Bioscience
Great.
Application Area:Analyze total methylation level in tissue samples
"This product is nothing but great; easy to perform the assay, and consistent results. The only issue is, customer service. Initially, particular reagent didn't work, so we requested a replacement. However, no replacement so far. Nonetheless, most reagents are perfect."
Status:
ReviewerMember since: 2016
Organization: MIDDLE EAST TECHNICAL UNIVERSITY
Easy to use kit, with low sensitivity and nice price.
Application Area:Global DNA methylation in bacteria
"It is a user friendly kit to detect global methylation level. However, the sensitivity was low especially for bacteria. The standards worked well but my samples showed zero methylation which is against the nature :) When I used another kit (different manufacturer) I have obtained reasonable results confirming the literature. I liked the good correlation and low error bars between the replicates of standards, which is serious problem in other kits. To summarize, it should be improved a lot to increase the reliability. The price is reasonable compared with the other manufacturer’s methylation kits."
Status:
ReviewerMember since: 2016
Organization: McGill University
Streamlined workflow
Application Area:cytosine modifications
"This product is efficient in estimating global levels of 5mC in DNA from multiple tissue types. The workflow is straightforward and can be completely in several hours."
The ability to efficiently detect and quantify DNA methylation (i.e., 5-methylcytosine) has become essential for epigenetic-based research. To date, a number of methods have been developed for this purpose including high-performance capillary electrophoresis, bisulfite sequencing, and methylated DNA immunoprecipitation.
The 5-mC DNA ELISA Kit is a convenient and powerful tool that allows the researcher to accurately quantitate 5-mC in any DNA sample in less than 3 hours. The kit features a unique Anti-5-Methylcytosine monoclonal antibody that is both sensitive and specific for 5-mC. The assay is compatible with a wide range of input DNA from vertebrate, plant, and microbial sources as well as PCR amplicons and fragmented DNA. Percent 5-mC in a DNA sample can be accurately quantified from a standard curve generated with specially designed controls included with the kit. Also, the fast, streamlined workflow is ideal for high-throughput analyses.