ZenoTOF 7600 system with SWATH DIA
A high-resolution mass spectrometry solution that combines powerful MS/MS sensitivity, fragmentation technology, and a step-change in data-independent acquisition.
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Marking a significant step-change in data-independent acquisition, Zeno SWATH DIA, exclusively on the new ZenoTOF 7600 system from SCIEX, delivers unprecedented levels of analyte identification and quantification. Harnessing the sensitivity gains provided by Zeno trap technology, MS/MS duty cycles of nearly >90% are now achievable for all ions, regardless of mass, change, or LC flow rate. Providing sensitivity gains of 4- to 20-fold without compromising other vital performance specifications such as resolution, mass accuracy, dynamic range and/or speed, the Zeno SWATH DIA workflow enables truly widespread and comprehensive analyte coverage at new depths of detection and higher throughput.
The ZenoTOF 7600 system is a high-resolution mass spectrometry solution that combines powerful MS/MS sensitivity, unique orthogonal fragmentation via electron activated dissociation (EAD) technology and a boost in SWATH DIA, allowing you to gain maximal information from each precious sample.
Benefits
- Characterize large molecules including post-translational modifications
- Elucidate positional isomers on small molecules and lipids
- Identify and quantify up to 2x as many proteins with traditional SWATH DIA approaches
- Shorten run-times to as little as 5 minutes with minimal compromise in proteome coverage
Reproducibility of SWATH™ MS Analysis and Implications for System Biology Studies
In system biology studies, collecting complete quantitative data is crucial for the construction and validation of the network models. Thus it is essential for the techniques applied to consistently, accurately and sensitively quantify a wide range of different species, especially the interactors that are located at the interfaces of the networks. This application note presents the analysis of a complex mixture with SWATH™-MS methods and evaluate the reproducibility of identification and quantification of a large number of peptides and proteins.
Comprehensive Metabolite Identification - Analysis of HepatoPac® Co-cultured Hepatocytes using SWATH™
The determination of metabolic stability and the accurate prediction of major human metabolites are critical for the optimization of drug candidates but traditional models for evaluating drug metabolism are short-lived and have a limited applicability for monitoring multi-generation metabolites or drug clearance kinetics. Furthermore, traditional LC/MS instrumentation approaches lack the sensitivity to measure metabolites within the therapeutic dosing range, often resulting in the need to generate metabolite profiling and drug clearance data from separate samples. This application note demonstrates the identification and quantitation of metabolites, as well as evaluated drug clearance kinetics from the same sample. Multi-generational drug metabolites obtained from a multi-species hepatocyte co-culture model (HepatoPac) were analyzed on a high-resolution TripleTOF® 6600 instrument with data-independent SWATH acquisition for simultaneous metabolite ID and relative quantitation across multiple time points.
